Experiment: LB_plus_SM_buffer with 6F2_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1886 and GFF1887 are separated by 30 nucleotides GFF1887 and GFF1888 overlap by 4 nucleotides GFF1888 and GFF1889 are separated by 2 nucleotides
GFF1886 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 78,287 to 81,013
GFF1886
GFF1887 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 81,044 to 82,381
GFF1887
GFF1888 - NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3), at 82,378 to 82,878
GFF1888
GFF1889 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 82,881 to 84,683
GFF1889
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 80.199 kb on + strand, within GFF1886 at 80.199 kb on + strand, within GFF1886 at 80.199 kb on + strand, within GFF1886 at 80.200 kb on - strand, within GFF1886 at 80.309 kb on - strand, within GFF1886 at 80.476 kb on - strand, within GFF1886 at 80.665 kb on + strand, within GFF1886 at 80.786 kb on - strand at 80.791 kb on + strand at 80.919 kb on + strand at 80.927 kb on - strand at 80.927 kb on - strand at 80.927 kb on - strand at 80.955 kb on - strand at 81.145 kb on + strand at 81.145 kb on + strand at 81.319 kb on + strand, within GFF1887 at 81.319 kb on + strand, within GFF1887 at 81.960 kb on - strand, within GFF1887 at 81.974 kb on - strand, within GFF1887 at 81.996 kb on + strand, within GFF1887 at 81.997 kb on - strand, within GFF1887 at 81.997 kb on - strand, within GFF1887 at 82.036 kb on + strand, within GFF1887 at 82.036 kb on + strand, within GFF1887 at 82.037 kb on - strand, within GFF1887 at 82.086 kb on + strand, within GFF1887 at 82.653 kb on - strand, within GFF1888 at 82.653 kb on - strand, within GFF1888 at 82.712 kb on + strand, within GFF1888 at 82.813 kb on + strand, within GFF1888 at 82.814 kb on - strand, within GFF1888 at 82.882 kb on + strand at 82.901 kb on + strand at 83.021 kb on + strand at 83.022 kb on - strand at 83.045 kb on - strand at 83.047 kb on + strand at 83.226 kb on - strand, within GFF1889 at 83.293 kb on + strand, within GFF1889 at 83.293 kb on + strand, within GFF1889 at 83.293 kb on + strand, within GFF1889 at 83.293 kb on + strand, within GFF1889 at 83.294 kb on - strand, within GFF1889 at 83.366 kb on + strand, within GFF1889
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.001875 MOI remove 80,199 + GFF1886 0.70 -0.3 80,199 + GFF1886 0.70 -1.3 80,199 + GFF1886 0.70 -1.8 80,200 - GFF1886 0.70 -0.9 80,309 - GFF1886 0.74 -2.4 80,476 - GFF1886 0.80 +0.3 80,665 + GFF1886 0.87 -1.3 80,786 - -1.2 80,791 + -0.5 80,919 + -2.7 80,927 - -1.1 80,927 - -1.7 80,927 - -1.6 80,955 - -2.2 81,145 + -2.8 81,145 + -0.8 81,319 + GFF1887 0.21 -1.5 81,319 + GFF1887 0.21 -1.8 81,960 - GFF1887 0.68 -2.4 81,974 - GFF1887 0.70 -2.3 81,996 + GFF1887 0.71 -1.2 81,997 - GFF1887 0.71 -2.0 81,997 - GFF1887 0.71 -2.8 82,036 + GFF1887 0.74 -1.8 82,036 + GFF1887 0.74 -1.7 82,037 - GFF1887 0.74 -1.5 82,086 + GFF1887 0.78 -1.2 82,653 - GFF1888 0.55 -0.7 82,653 - GFF1888 0.55 -1.2 82,712 + GFF1888 0.67 -2.7 82,813 + GFF1888 0.87 -1.5 82,814 - GFF1888 0.87 -0.4 82,882 + -1.3 82,901 + -1.9 83,021 + -1.2 83,022 - -1.9 83,045 - -1.4 83,047 + -2.0 83,226 - GFF1889 0.19 -1.4 83,293 + GFF1889 0.23 -0.8 83,293 + GFF1889 0.23 +0.5 83,293 + GFF1889 0.23 -0.1 83,293 + GFF1889 0.23 -1.4 83,294 - GFF1889 0.23 -1.3 83,366 + GFF1889 0.27 -1.7
Or see this region's nucleotide sequence