Experiment: L-Lysine 60 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09460 and PS417_09465 are separated by 9 nucleotides PS417_09465 and PS417_09470 are separated by 399 nucleotides PS417_09470 and PS417_09475 are separated by 233 nucleotides
PS417_09460: PS417_09460 - alkanesulfonate monooxygenase, at 2,048,616 to 2,049,758
_09460
PS417_09465: PS417_09465 - FMN reductase, at 2,049,768 to 2,050,331
_09465
PS417_09470: PS417_09470 - hypothetical protein, at 2,050,731 to 2,051,000
_09470
PS417_09475: PS417_09475 - class V aminotransferase, at 2,051,234 to 2,052,394
_09475
Position (kb)
2049
2050
2051 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2049.184 kb on + strand, within PS417_09460 at 2049.184 kb on + strand, within PS417_09460 at 2049.339 kb on + strand at 2049.380 kb on - strand, within PS417_09460 at 2049.674 kb on - strand at 2049.716 kb on - strand at 2049.849 kb on + strand, within PS417_09465 at 2049.849 kb on + strand, within PS417_09465 at 2049.850 kb on - strand, within PS417_09465 at 2049.850 kb on - strand, within PS417_09465 at 2049.850 kb on - strand, within PS417_09465 at 2049.852 kb on + strand, within PS417_09465 at 2049.853 kb on - strand, within PS417_09465 at 2049.864 kb on + strand, within PS417_09465 at 2049.899 kb on - strand, within PS417_09465 at 2050.081 kb on + strand, within PS417_09465 at 2050.251 kb on + strand, within PS417_09465 at 2050.539 kb on + strand at 2050.539 kb on + strand at 2050.541 kb on + strand at 2050.542 kb on - strand at 2050.542 kb on - strand at 2050.646 kb on + strand at 2050.686 kb on - strand at 2050.819 kb on - strand, within PS417_09470 at 2050.888 kb on - strand, within PS417_09470 at 2051.119 kb on - strand at 2051.119 kb on - strand at 2051.119 kb on - strand at 2051.122 kb on + strand at 2051.138 kb on + strand at 2051.239 kb on + strand at 2051.239 kb on + strand at 2051.252 kb on + strand at 2051.261 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 60 mM remove 2,049,184 + PS417_09460 0.50 +3.2 2,049,184 + PS417_09460 0.50 +1.2 2,049,339 + -1.3 2,049,380 - PS417_09460 0.67 +0.1 2,049,674 - -0.6 2,049,716 - +1.4 2,049,849 + PS417_09465 0.14 -0.7 2,049,849 + PS417_09465 0.14 -1.0 2,049,850 - PS417_09465 0.15 -1.1 2,049,850 - PS417_09465 0.15 -0.1 2,049,850 - PS417_09465 0.15 -0.9 2,049,852 + PS417_09465 0.15 -0.2 2,049,853 - PS417_09465 0.15 +0.7 2,049,864 + PS417_09465 0.17 -1.3 2,049,899 - PS417_09465 0.23 +0.1 2,050,081 + PS417_09465 0.55 -0.9 2,050,251 + PS417_09465 0.86 -0.4 2,050,539 + -0.4 2,050,539 + +0.5 2,050,541 + +0.5 2,050,542 - -2.9 2,050,542 - -0.1 2,050,646 + -0.6 2,050,686 - -0.1 2,050,819 - PS417_09470 0.33 -0.2 2,050,888 - PS417_09470 0.58 +0.5 2,051,119 - -1.2 2,051,119 - -0.0 2,051,119 - -0.0 2,051,122 + -0.7 2,051,138 + -0.4 2,051,239 + +2.0 2,051,239 + +1.1 2,051,252 + +0.0 2,051,261 + -0.8
Or see this region's nucleotide sequence