Experiment: LB_plus_SM_buffer with 6F2_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1121 and GFF1122 are separated by 39 nucleotides GFF1122 and GFF1123 are separated by 455 nucleotides GFF1123 and GFF1124 are separated by 252 nucleotides
GFF1121 - Alpha-xylosidase (EC 3.2.1.-), at 309,156 to 311,195
GFF1121
GFF1122 - Xyloside transporter XynT, at 311,235 to 312,608
GFF1122
GFF1123 - SSU ribosomal protein S20p, at 313,064 to 313,249
GFF1123
GFF1124 - FIG01045144: hypothetical protein, at 313,502 to 313,648
GFF1124
Position (kb) 311
312
313 Strain fitness (log2 ratio) -1
0
1
2
3 at 310.374 kb on - strand, within GFF1121 at 310.393 kb on + strand, within GFF1121 at 310.550 kb on - strand, within GFF1121 at 310.662 kb on - strand, within GFF1121 at 310.754 kb on - strand, within GFF1121 at 310.946 kb on + strand, within GFF1121 at 310.976 kb on + strand, within GFF1121 at 311.187 kb on - strand at 311.187 kb on - strand at 311.288 kb on - strand at 311.348 kb on + strand at 311.474 kb on + strand, within GFF1122 at 311.475 kb on - strand, within GFF1122 at 311.475 kb on - strand, within GFF1122 at 311.507 kb on + strand, within GFF1122 at 311.510 kb on + strand, within GFF1122 at 311.688 kb on + strand, within GFF1122 at 311.708 kb on - strand, within GFF1122 at 311.800 kb on + strand, within GFF1122 at 311.891 kb on + strand, within GFF1122 at 312.006 kb on - strand, within GFF1122 at 312.387 kb on + strand, within GFF1122 at 312.400 kb on - strand, within GFF1122 at 312.400 kb on - strand, within GFF1122 at 312.411 kb on - strand, within GFF1122 at 312.615 kb on - strand at 313.418 kb on + strand at 313.419 kb on - strand at 313.490 kb on + strand at 313.518 kb on + strand, within GFF1124
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.001875 MOI remove 310,374 - GFF1121 0.60 -0.6 310,393 + GFF1121 0.61 +0.2 310,550 - GFF1121 0.68 -0.3 310,662 - GFF1121 0.74 -0.4 310,754 - GFF1121 0.78 +0.3 310,946 + GFF1121 0.88 -0.7 310,976 + GFF1121 0.89 -0.0 311,187 - -0.7 311,187 - +0.7 311,288 - +0.2 311,348 + -0.3 311,474 + GFF1122 0.17 +0.3 311,475 - GFF1122 0.17 +0.1 311,475 - GFF1122 0.17 -0.2 311,507 + GFF1122 0.20 +0.1 311,510 + GFF1122 0.20 +0.9 311,688 + GFF1122 0.33 +0.5 311,708 - GFF1122 0.34 +0.2 311,800 + GFF1122 0.41 +0.7 311,891 + GFF1122 0.48 -0.2 312,006 - GFF1122 0.56 -0.7 312,387 + GFF1122 0.84 -0.4 312,400 - GFF1122 0.85 +0.7 312,400 - GFF1122 0.85 +3.2 312,411 - GFF1122 0.86 +1.4 312,615 - +0.1 313,418 + +0.2 313,419 - -1.7 313,490 + -0.2 313,518 + GFF1124 0.11 +0.6
Or see this region's nucleotide sequence