Experiment: L-Lysine 60 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05185 and PS417_05190 are separated by 150 nucleotides PS417_05190 and PS417_05195 overlap by 8 nucleotides PS417_05195 and PS417_05200 are separated by 154 nucleotides PS417_05200 and PS417_05205 are separated by 51 nucleotides
PS417_05185: PS417_05185 - signal peptidase, at 1,151,593 to 1,152,447
_05185
PS417_05190: PS417_05190 - ribonuclease III, at 1,152,598 to 1,153,287
_05190
PS417_05195: PS417_05195 - GTPase Era, at 1,153,280 to 1,154,182
_05195
PS417_05200: PS417_05200 - DNA recombination protein RecO, at 1,154,337 to 1,155,026
_05200
PS417_05205: PS417_05205 - pyridoxine 5'-phosphate synthase, at 1,155,078 to 1,155,821
_05205
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-1
0
1
2 at 1152.445 kb on + strand at 1154.247 kb on + strand at 1154.248 kb on - strand at 1154.248 kb on - strand at 1154.313 kb on - strand at 1154.370 kb on + strand at 1154.371 kb on - strand at 1154.449 kb on + strand, within PS417_05200 at 1154.450 kb on - strand, within PS417_05200 at 1154.450 kb on - strand at 1154.579 kb on + strand, within PS417_05200 at 1154.608 kb on - strand, within PS417_05200 at 1154.608 kb on - strand, within PS417_05200 at 1154.820 kb on + strand, within PS417_05200 at 1155.037 kb on + strand at 1155.037 kb on + strand at 1155.038 kb on - strand at 1155.038 kb on - strand at 1155.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 60 mM remove 1,152,445 + +2.7 1,154,247 + -0.1 1,154,248 - +0.1 1,154,248 - +0.3 1,154,313 - -0.6 1,154,370 + -0.2 1,154,371 - +0.4 1,154,449 + PS417_05200 0.16 -1.5 1,154,450 - PS417_05200 0.16 -0.7 1,154,450 - +1.0 1,154,579 + PS417_05200 0.35 +0.1 1,154,608 - PS417_05200 0.39 +0.7 1,154,608 - PS417_05200 0.39 +0.3 1,154,820 + PS417_05200 0.70 +1.6 1,155,037 + -1.0 1,155,037 + +0.3 1,155,038 - +0.0 1,155,038 - +0.4 1,155,038 - +0.3
Or see this region's nucleotide sequence