Strain Fitness in Pseudomonas simiae WCS417 around PS417_05195

Experiment: L-Lysine 60 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_05185 and PS417_05190 are separated by 150 nucleotidesPS417_05190 and PS417_05195 overlap by 8 nucleotidesPS417_05195 and PS417_05200 are separated by 154 nucleotidesPS417_05200 and PS417_05205 are separated by 51 nucleotides PS417_05185: PS417_05185 - signal peptidase, at 1,151,593 to 1,152,447 _05185 PS417_05190: PS417_05190 - ribonuclease III, at 1,152,598 to 1,153,287 _05190 PS417_05195: PS417_05195 - GTPase Era, at 1,153,280 to 1,154,182 _05195 PS417_05200: PS417_05200 - DNA recombination protein RecO, at 1,154,337 to 1,155,026 _05200 PS417_05205: PS417_05205 - pyridoxine 5'-phosphate synthase, at 1,155,078 to 1,155,821 _05205 Position (kb) 1153 1154 1155Strain fitness (log2 ratio) -1 0 1 2at 1152.445 kb on + strandat 1154.247 kb on + strandat 1154.248 kb on - strandat 1154.248 kb on - strandat 1154.313 kb on - strandat 1154.370 kb on + strandat 1154.371 kb on - strandat 1154.449 kb on + strand, within PS417_05200at 1154.450 kb on - strand, within PS417_05200at 1154.450 kb on - strandat 1154.579 kb on + strand, within PS417_05200at 1154.608 kb on - strand, within PS417_05200at 1154.608 kb on - strand, within PS417_05200at 1154.820 kb on + strand, within PS417_05200at 1155.037 kb on + strandat 1155.037 kb on + strandat 1155.038 kb on - strandat 1155.038 kb on - strandat 1155.038 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine 60 mM
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1,152,445 + +2.7
1,154,247 + -0.1
1,154,248 - +0.1
1,154,248 - +0.3
1,154,313 - -0.6
1,154,370 + -0.2
1,154,371 - +0.4
1,154,449 + PS417_05200 0.16 -1.5
1,154,450 - PS417_05200 0.16 -0.7
1,154,450 - +1.0
1,154,579 + PS417_05200 0.35 +0.1
1,154,608 - PS417_05200 0.39 +0.7
1,154,608 - PS417_05200 0.39 +0.3
1,154,820 + PS417_05200 0.70 +1.6
1,155,037 + -1.0
1,155,037 + +0.3
1,155,038 - +0.0
1,155,038 - +0.4
1,155,038 - +0.3

Or see this region's nucleotide sequence