Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgcR and sgcE are separated by 16 nucleotides sgcE and sgcA are separated by 11 nucleotides sgcA and ryjB overlap by 4 nucleotides ryjB and sgcQ are separated by 44 nucleotides
b4300: sgcR - KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI), at 4,524,129 to 4,524,911
sgcR
b4301: sgcE - ribulose-phosphate 3-epimerase (NCBI), at 4,524,928 to 4,525,560
sgcE
b4302: sgcA - KpLE2 phage-like element; predicted phosphotransferase enzyme IIA component (NCBI), at 4,525,572 to 4,526,003
sgcA
b4624: ryjB - Novel sRNA, function unknown (RefSeq), at 4,526,000 to 4,526,089
ryjB
b4303: sgcQ - KpLE2 phage-like element; predicted nucleoside triphosphatase (NCBI), at 4,526,134 to 4,526,940
sgcQ
Position (kb)
4524
4525
4526 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4523.937 kb on - strand at 4523.988 kb on - strand at 4523.996 kb on - strand at 4524.000 kb on + strand at 4524.000 kb on + strand at 4524.003 kb on + strand at 4524.008 kb on - strand at 4524.008 kb on - strand at 4524.008 kb on - strand at 4524.008 kb on - strand at 4524.008 kb on - strand at 4524.009 kb on + strand at 4524.009 kb on + strand at 4524.009 kb on + strand at 4524.009 kb on + strand at 4524.009 kb on - strand at 4524.011 kb on + strand at 4524.011 kb on + strand at 4524.011 kb on + strand at 4524.011 kb on + strand at 4524.011 kb on + strand at 4524.011 kb on - strand at 4524.017 kb on - strand at 4524.017 kb on - strand at 4524.017 kb on - strand at 4524.017 kb on - strand at 4524.017 kb on - strand at 4524.017 kb on - strand at 4524.017 kb on - strand at 4524.018 kb on - strand at 4524.018 kb on - strand at 4524.019 kb on - strand at 4524.019 kb on - strand at 4524.019 kb on - strand at 4524.019 kb on - strand at 4524.027 kb on - strand at 4524.029 kb on + strand at 4524.125 kb on + strand at 4524.125 kb on + strand at 4524.132 kb on - strand at 4524.132 kb on - strand at 4524.166 kb on - strand at 4524.168 kb on - strand at 4524.178 kb on - strand at 4524.178 kb on - strand at 4524.178 kb on - strand at 4524.178 kb on - strand at 4524.180 kb on - strand at 4524.180 kb on - strand at 4524.180 kb on - strand at 4524.180 kb on - strand at 4524.182 kb on + strand at 4524.188 kb on - strand at 4524.188 kb on - strand at 4524.189 kb on + strand at 4524.190 kb on + strand at 4524.190 kb on - strand at 4524.190 kb on - strand at 4524.190 kb on - strand at 4524.196 kb on + strand at 4524.198 kb on - strand at 4524.198 kb on - strand at 4524.198 kb on - strand at 4524.198 kb on - strand at 4524.202 kb on - strand at 4524.202 kb on - strand at 4524.203 kb on - strand at 4524.207 kb on + strand at 4524.207 kb on - strand at 4524.207 kb on - strand at 4524.215 kb on - strand, within sgcR at 4524.228 kb on + strand, within sgcR at 4524.228 kb on + strand, within sgcR at 4524.229 kb on - strand, within sgcR at 4524.239 kb on + strand, within sgcR at 4524.247 kb on - strand, within sgcR at 4524.252 kb on + strand, within sgcR at 4524.252 kb on + strand, within sgcR at 4524.282 kb on + strand, within sgcR at 4524.282 kb on + strand, within sgcR at 4524.360 kb on - strand, within sgcR at 4524.377 kb on - strand, within sgcR at 4524.385 kb on - strand, within sgcR at 4524.401 kb on + strand, within sgcR at 4524.436 kb on + strand, within sgcR at 4524.436 kb on + strand, within sgcR at 4524.471 kb on - strand, within sgcR at 4524.471 kb on - strand, within sgcR at 4524.476 kb on + strand, within sgcR at 4524.486 kb on - strand, within sgcR at 4524.503 kb on + strand, within sgcR at 4524.503 kb on + strand, within sgcR at 4524.579 kb on - strand, within sgcR at 4524.723 kb on - strand, within sgcR at 4524.723 kb on - strand, within sgcR at 4524.726 kb on - strand, within sgcR at 4524.771 kb on - strand, within sgcR at 4524.831 kb on + strand, within sgcR at 4524.847 kb on - strand at 4524.881 kb on + strand at 4524.898 kb on - strand at 4524.924 kb on + strand at 4524.968 kb on + strand at 4524.968 kb on + strand at 4524.976 kb on + strand at 4525.061 kb on - strand, within sgcE at 4525.078 kb on - strand, within sgcE at 4525.167 kb on - strand, within sgcE at 4525.217 kb on - strand, within sgcE at 4525.220 kb on + strand, within sgcE at 4525.220 kb on + strand, within sgcE at 4525.290 kb on - strand, within sgcE at 4525.315 kb on - strand, within sgcE at 4525.315 kb on - strand, within sgcE at 4525.363 kb on + strand, within sgcE at 4525.462 kb on + strand, within sgcE at 4525.492 kb on + strand, within sgcE at 4525.492 kb on + strand, within sgcE at 4525.519 kb on + strand at 4525.549 kb on + strand at 4525.559 kb on + strand at 4525.565 kb on - strand at 4525.632 kb on - strand, within sgcA at 4525.632 kb on - strand, within sgcA at 4525.650 kb on + strand, within sgcA at 4525.663 kb on - strand, within sgcA at 4525.756 kb on + strand, within sgcA at 4525.761 kb on + strand, within sgcA at 4525.805 kb on - strand, within sgcA at 4525.805 kb on - strand, within sgcA at 4525.844 kb on + strand, within sgcA at 4525.888 kb on + strand, within sgcA at 4525.888 kb on + strand, within sgcA at 4525.896 kb on + strand, within sgcA at 4525.896 kb on + strand, within sgcA at 4525.914 kb on + strand, within sgcA at 4525.971 kb on - strand at 4526.051 kb on + strand, within ryjB at 4526.140 kb on + strand at 4526.140 kb on + strand at 4526.140 kb on + strand at 4526.164 kb on + strand at 4526.174 kb on + strand at 4526.174 kb on + strand at 4526.254 kb on + strand, within sgcQ at 4526.254 kb on + strand, within sgcQ at 4526.257 kb on + strand, within sgcQ at 4526.298 kb on + strand, within sgcQ at 4526.338 kb on - strand at 4526.356 kb on + strand, within sgcQ at 4526.356 kb on + strand, within sgcQ at 4526.388 kb on + strand, within sgcQ at 4526.448 kb on + strand, within sgcQ at 4526.448 kb on + strand, within sgcQ at 4526.460 kb on + strand, within sgcQ at 4526.461 kb on + strand, within sgcQ at 4526.468 kb on - strand, within sgcQ at 4526.468 kb on - strand, within sgcQ
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 4,523,937 - -0.2 4,523,988 - +0.3 4,523,996 - -0.7 4,524,000 + -0.2 4,524,000 + +0.7 4,524,003 + -0.1 4,524,008 - -0.2 4,524,008 - -0.1 4,524,008 - -0.2 4,524,008 - -0.3 4,524,008 - -0.0 4,524,009 + +0.4 4,524,009 + -0.2 4,524,009 + -0.6 4,524,009 + +0.2 4,524,009 - -0.5 4,524,011 + +0.7 4,524,011 + +0.2 4,524,011 + +0.5 4,524,011 + +1.7 4,524,011 + +0.1 4,524,011 - +0.4 4,524,017 - -0.2 4,524,017 - +0.0 4,524,017 - +0.3 4,524,017 - +0.1 4,524,017 - -0.6 4,524,017 - +0.3 4,524,017 - +0.2 4,524,018 - -0.2 4,524,018 - +1.4 4,524,019 - +0.6 4,524,019 - +0.3 4,524,019 - +0.2 4,524,019 - -1.0 4,524,027 - -0.8 4,524,029 + +0.3 4,524,125 + -0.5 4,524,125 + -1.3 4,524,132 - -0.1 4,524,132 - -0.5 4,524,166 - -0.9 4,524,168 - -0.6 4,524,178 - -0.7 4,524,178 - +0.3 4,524,178 - -0.7 4,524,178 - +0.8 4,524,180 - -1.0 4,524,180 - +0.3 4,524,180 - -0.3 4,524,180 - +1.0 4,524,182 + -0.6 4,524,188 - +0.3 4,524,188 - +0.5 4,524,189 + -0.0 4,524,190 + -0.7 4,524,190 - -1.2 4,524,190 - +0.4 4,524,190 - -0.1 4,524,196 + +0.2 4,524,198 - -0.2 4,524,198 - -0.3 4,524,198 - -0.3 4,524,198 - +0.9 4,524,202 - -1.9 4,524,202 - -0.8 4,524,203 - +0.5 4,524,207 + -1.2 4,524,207 - +0.4 4,524,207 - -0.0 4,524,215 - sgcR b4300 0.11 +0.5 4,524,228 + sgcR b4300 0.13 +0.6 4,524,228 + sgcR b4300 0.13 -0.5 4,524,229 - sgcR b4300 0.13 -1.8 4,524,239 + sgcR b4300 0.14 +0.3 4,524,247 - sgcR b4300 0.15 +0.1 4,524,252 + sgcR b4300 0.16 +0.9 4,524,252 + sgcR b4300 0.16 -0.7 4,524,282 + sgcR b4300 0.20 +0.5 4,524,282 + sgcR b4300 0.20 +0.4 4,524,360 - sgcR b4300 0.30 +0.2 4,524,377 - sgcR b4300 0.32 +0.1 4,524,385 - sgcR b4300 0.33 -0.5 4,524,401 + sgcR b4300 0.35 -0.7 4,524,436 + sgcR b4300 0.39 -0.2 4,524,436 + sgcR b4300 0.39 -0.6 4,524,471 - sgcR b4300 0.44 +0.5 4,524,471 - sgcR b4300 0.44 -0.2 4,524,476 + sgcR b4300 0.44 -0.1 4,524,486 - sgcR b4300 0.46 -0.2 4,524,503 + sgcR b4300 0.48 +0.0 4,524,503 + sgcR b4300 0.48 +0.3 4,524,579 - sgcR b4300 0.57 +0.4 4,524,723 - sgcR b4300 0.76 -3.2 4,524,723 - sgcR b4300 0.76 -0.6 4,524,726 - sgcR b4300 0.76 -0.3 4,524,771 - sgcR b4300 0.82 -1.2 4,524,831 + sgcR b4300 0.90 -0.4 4,524,847 - +0.0 4,524,881 + +0.2 4,524,898 - +0.1 4,524,924 + +0.2 4,524,968 + -1.9 4,524,968 + +0.4 4,524,976 + +0.1 4,525,061 - sgcE b4301 0.21 +0.7 4,525,078 - sgcE b4301 0.24 -0.5 4,525,167 - sgcE b4301 0.38 -0.7 4,525,217 - sgcE b4301 0.46 +0.2 4,525,220 + sgcE b4301 0.46 +0.9 4,525,220 + sgcE b4301 0.46 -0.3 4,525,290 - sgcE b4301 0.57 +0.4 4,525,315 - sgcE b4301 0.61 -1.5 4,525,315 - sgcE b4301 0.61 +0.7 4,525,363 + sgcE b4301 0.69 +0.0 4,525,462 + sgcE b4301 0.84 +0.1 4,525,492 + sgcE b4301 0.89 -0.2 4,525,492 + sgcE b4301 0.89 +0.2 4,525,519 + +0.1 4,525,549 + +0.1 4,525,559 + -0.0 4,525,565 - +0.7 4,525,632 - sgcA b4302 0.14 +0.4 4,525,632 - sgcA b4302 0.14 +0.5 4,525,650 + sgcA b4302 0.18 +0.6 4,525,663 - sgcA b4302 0.21 -0.1 4,525,756 + sgcA b4302 0.43 -0.5 4,525,761 + sgcA b4302 0.44 +0.2 4,525,805 - sgcA b4302 0.54 +0.5 4,525,805 - sgcA b4302 0.54 +0.8 4,525,844 + sgcA b4302 0.63 -0.8 4,525,888 + sgcA b4302 0.73 -0.1 4,525,888 + sgcA b4302 0.73 -0.3 4,525,896 + sgcA b4302 0.75 +0.1 4,525,896 + sgcA b4302 0.75 -0.4 4,525,914 + sgcA b4302 0.79 -0.4 4,525,971 - -0.5 4,526,051 + ryjB b4624 0.57 +0.1 4,526,140 + +0.9 4,526,140 + +0.1 4,526,140 + -1.3 4,526,164 + -0.3 4,526,174 + +0.2 4,526,174 + +0.2 4,526,254 + sgcQ b4303 0.15 -1.1 4,526,254 + sgcQ b4303 0.15 +0.7 4,526,257 + sgcQ b4303 0.15 +0.3 4,526,298 + sgcQ b4303 0.20 +0.4 4,526,338 - -0.1 4,526,356 + sgcQ b4303 0.28 +0.6 4,526,356 + sgcQ b4303 0.28 -0.3 4,526,388 + sgcQ b4303 0.31 -0.3 4,526,448 + sgcQ b4303 0.39 -1.0 4,526,448 + sgcQ b4303 0.39 +0.2 4,526,460 + sgcQ b4303 0.40 -0.8 4,526,461 + sgcQ b4303 0.41 +0.7 4,526,468 - sgcQ b4303 0.41 -0.7 4,526,468 - sgcQ b4303 0.41 +1.0
Or see this region's nucleotide sequence