Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sohA and yhaV overlap by 1 nucleotides yhaV and agaR are separated by 54 nucleotides agaR and kbaZ are separated by 248 nucleotides
b3129: sohA - predicted regulator (NCBI), at 3,275,024 to 3,275,359
sohA
b3130: yhaV - hypothetical protein (NCBI), at 3,275,359 to 3,275,823
yhaV
b3131: agaR - DNA-binding transcriptional dual regulator (NCBI), at 3,275,878 to 3,276,687
agaR
b3132: kbaZ - tagatose 6-phosphate aldolase 1, kbaZ subunit (NCBI), at 3,276,936 to 3,278,216
kbaZ
Position (kb)
3275
3276
3277 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3275.046 kb on + strand at 3275.046 kb on + strand at 3275.049 kb on - strand at 3275.049 kb on - strand at 3275.049 kb on - strand at 3275.051 kb on + strand at 3275.051 kb on + strand at 3275.057 kb on - strand at 3275.059 kb on - strand, within sohA at 3275.063 kb on + strand, within sohA at 3275.098 kb on + strand, within sohA at 3275.108 kb on + strand, within sohA at 3275.108 kb on + strand, within sohA at 3275.118 kb on + strand, within sohA at 3275.118 kb on + strand, within sohA at 3275.318 kb on - strand, within sohA at 3275.318 kb on - strand, within sohA at 3275.392 kb on + strand at 3275.392 kb on + strand at 3275.392 kb on + strand at 3275.400 kb on - strand at 3275.400 kb on - strand at 3275.420 kb on - strand, within yhaV at 3275.427 kb on + strand, within yhaV at 3275.451 kb on + strand, within yhaV at 3275.512 kb on + strand, within yhaV at 3275.536 kb on + strand, within yhaV at 3275.596 kb on + strand, within yhaV at 3275.642 kb on - strand, within yhaV at 3275.642 kb on - strand, within yhaV at 3275.678 kb on + strand, within yhaV at 3275.685 kb on - strand, within yhaV at 3275.685 kb on - strand, within yhaV at 3275.686 kb on - strand, within yhaV at 3275.686 kb on - strand, within yhaV at 3275.690 kb on - strand, within yhaV at 3275.735 kb on + strand, within yhaV at 3275.741 kb on - strand, within yhaV at 3275.781 kb on - strand at 3275.781 kb on - strand at 3276.109 kb on + strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.170 kb on + strand, within agaR at 3276.242 kb on - strand, within agaR at 3276.242 kb on - strand at 3276.270 kb on + strand, within agaR at 3276.447 kb on - strand, within agaR at 3276.447 kb on - strand, within agaR at 3276.560 kb on - strand, within agaR at 3276.602 kb on - strand, within agaR at 3276.625 kb on - strand at 3276.635 kb on - strand at 3276.643 kb on + strand at 3276.762 kb on - strand at 3276.790 kb on - strand at 3277.028 kb on - strand at 3277.120 kb on - strand, within kbaZ at 3277.220 kb on - strand, within kbaZ at 3277.261 kb on - strand, within kbaZ at 3277.338 kb on - strand, within kbaZ at 3277.338 kb on - strand, within kbaZ at 3277.393 kb on + strand, within kbaZ at 3277.393 kb on + strand, within kbaZ at 3277.419 kb on + strand, within kbaZ at 3277.443 kb on - strand, within kbaZ at 3277.443 kb on - strand, within kbaZ at 3277.477 kb on + strand, within kbaZ at 3277.602 kb on - strand, within kbaZ at 3277.603 kb on + strand, within kbaZ at 3277.603 kb on + strand, within kbaZ at 3277.647 kb on + strand, within kbaZ at 3277.647 kb on + strand, within kbaZ
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 3,275,046 + +1.1 3,275,046 + +1.6 3,275,049 - -0.5 3,275,049 - +0.9 3,275,049 - +0.5 3,275,051 + -0.6 3,275,051 + +0.6 3,275,057 - +0.3 3,275,059 - sohA b3129 0.10 +1.3 3,275,063 + sohA b3129 0.12 -1.9 3,275,098 + sohA b3129 0.22 +1.1 3,275,108 + sohA b3129 0.25 +1.0 3,275,108 + sohA b3129 0.25 -0.1 3,275,118 + sohA b3129 0.28 +1.7 3,275,118 + sohA b3129 0.28 +0.0 3,275,318 - sohA b3129 0.88 +1.2 3,275,318 - sohA b3129 0.88 -0.0 3,275,392 + -0.5 3,275,392 + +0.1 3,275,392 + -0.4 3,275,400 - +0.5 3,275,400 - +1.0 3,275,420 - yhaV b3130 0.13 -0.2 3,275,427 + yhaV b3130 0.15 +0.4 3,275,451 + yhaV b3130 0.20 +0.3 3,275,512 + yhaV b3130 0.33 +0.2 3,275,536 + yhaV b3130 0.38 +0.5 3,275,596 + yhaV b3130 0.51 +0.1 3,275,642 - yhaV b3130 0.61 +1.2 3,275,642 - yhaV b3130 0.61 +1.3 3,275,678 + yhaV b3130 0.69 +1.0 3,275,685 - yhaV b3130 0.70 -0.1 3,275,685 - yhaV b3130 0.70 +1.1 3,275,686 - yhaV b3130 0.70 -0.1 3,275,686 - yhaV b3130 0.70 -0.4 3,275,690 - yhaV b3130 0.71 +0.5 3,275,735 + yhaV b3130 0.81 +0.8 3,275,741 - yhaV b3130 0.82 +0.3 3,275,781 - +0.6 3,275,781 - -0.4 3,276,109 + agaR b3131 0.29 -2.3 3,276,131 - agaR b3131 0.31 -2.1 3,276,131 - agaR b3131 0.31 -2.5 3,276,131 - agaR b3131 0.31 -0.7 3,276,170 + agaR b3131 0.36 -1.2 3,276,242 - agaR b3131 0.45 -3.0 3,276,242 - -0.1 3,276,270 + agaR b3131 0.48 -2.1 3,276,447 - agaR b3131 0.70 -1.3 3,276,447 - agaR b3131 0.70 -2.9 3,276,560 - agaR b3131 0.84 -1.2 3,276,602 - agaR b3131 0.89 -2.7 3,276,625 - -1.7 3,276,635 - -1.8 3,276,643 + -2.4 3,276,762 - +0.1 3,276,790 - +0.5 3,277,028 - +0.2 3,277,120 - kbaZ b3132 0.14 -0.2 3,277,220 - kbaZ b3132 0.22 -1.6 3,277,261 - kbaZ b3132 0.25 +0.5 3,277,338 - kbaZ b3132 0.31 +0.5 3,277,338 - kbaZ b3132 0.31 -0.6 3,277,393 + kbaZ b3132 0.36 -1.6 3,277,393 + kbaZ b3132 0.36 +1.0 3,277,419 + kbaZ b3132 0.38 -0.3 3,277,443 - kbaZ b3132 0.40 +0.0 3,277,443 - kbaZ b3132 0.40 -0.8 3,277,477 + kbaZ b3132 0.42 +0.4 3,277,602 - kbaZ b3132 0.52 -0.6 3,277,603 + kbaZ b3132 0.52 -1.0 3,277,603 + kbaZ b3132 0.52 +0.7 3,277,647 + kbaZ b3132 0.56 -0.2 3,277,647 + kbaZ b3132 0.56 +0.4
Or see this region's nucleotide sequence