Strain Fitness in Escherichia coli BW25113 around b2704

Experiment: 2,4-Dinitrophenol 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsrlA and srlE overlap by 4 nucleotidessrlE and srlB are separated by 10 nucleotidessrlB and srlD are separated by 3 nucleotidessrlD and gutM are separated by 104 nucleotides b2702: srlA - glucitol/sorbitol-specific enzyme IIC component of PTS (NCBI), at 2,823,854 to 2,824,417 srlA b2703: srlE - glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI), at 2,824,414 to 2,825,373 srlE b2704: srlB - glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI), at 2,825,384 to 2,825,755 srlB b2705: srlD - 3-ketoacyl-(acyl-carrier-protein) reductase (NCBI), at 2,825,759 to 2,826,538 srlD b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002 gutM Position (kb) 2825 2826Strain fitness (log2 ratio) -2 -1 0 1 2at 2824.412 kb on - strandat 2824.412 kb on - strandat 2824.460 kb on + strandat 2824.683 kb on - strand, within srlEat 2824.736 kb on - strand, within srlEat 2824.775 kb on + strand, within srlEat 2824.933 kb on - strand, within srlEat 2824.938 kb on - strand, within srlEat 2824.938 kb on - strand, within srlEat 2824.969 kb on + strand, within srlEat 2824.969 kb on + strand, within srlEat 2824.983 kb on + strand, within srlEat 2824.983 kb on + strand, within srlEat 2825.009 kb on + strand, within srlEat 2825.113 kb on - strand, within srlEat 2825.179 kb on + strand, within srlEat 2825.263 kb on + strand, within srlEat 2825.263 kb on + strand, within srlEat 2825.296 kb on + strandat 2825.569 kb on + strand, within srlBat 2825.599 kb on - strand, within srlBat 2825.631 kb on + strand, within srlBat 2825.631 kb on + strand, within srlBat 2825.641 kb on + strand, within srlBat 2825.689 kb on - strand, within srlBat 2825.709 kb on + strand, within srlBat 2825.734 kb on + strandat 2825.807 kb on + strandat 2825.921 kb on - strand, within srlDat 2825.949 kb on - strand, within srlDat 2825.949 kb on - strand, within srlDat 2825.973 kb on + strand, within srlDat 2826.044 kb on - strand, within srlDat 2826.210 kb on + strand, within srlDat 2826.210 kb on + strand, within srlDat 2826.215 kb on + strand, within srlDat 2826.218 kb on - strand, within srlDat 2826.218 kb on - strand, within srlDat 2826.245 kb on + strand, within srlDat 2826.318 kb on - strand, within srlDat 2826.397 kb on + strand, within srlDat 2826.397 kb on + strand, within srlDat 2826.397 kb on + strand, within srlDat 2826.397 kb on + strand, within srlDat 2826.474 kb on + strandat 2826.474 kb on + strandat 2826.482 kb on - strandat 2826.490 kb on - strandat 2826.490 kb on - strandat 2826.613 kb on - strandat 2826.613 kb on - strandat 2826.613 kb on - strandat 2826.646 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM
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2,824,412 - +0.5
2,824,412 - +0.8
2,824,460 + +0.5
2,824,683 - srlE b2703 0.28 +0.7
2,824,736 - srlE b2703 0.34 +0.4
2,824,775 + srlE b2703 0.38 +0.2
2,824,933 - srlE b2703 0.54 +0.3
2,824,938 - srlE b2703 0.55 -0.7
2,824,938 - srlE b2703 0.55 +1.0
2,824,969 + srlE b2703 0.58 -0.1
2,824,969 + srlE b2703 0.58 +0.9
2,824,983 + srlE b2703 0.59 +0.4
2,824,983 + srlE b2703 0.59 +0.7
2,825,009 + srlE b2703 0.62 -0.3
2,825,113 - srlE b2703 0.73 -0.5
2,825,179 + srlE b2703 0.80 -1.0
2,825,263 + srlE b2703 0.88 +0.8
2,825,263 + srlE b2703 0.88 +0.8
2,825,296 + -0.8
2,825,569 + srlB b2704 0.50 +0.1
2,825,599 - srlB b2704 0.58 +0.1
2,825,631 + srlB b2704 0.66 +0.4
2,825,631 + srlB b2704 0.66 +1.8
2,825,641 + srlB b2704 0.69 -0.5
2,825,689 - srlB b2704 0.82 -0.4
2,825,709 + srlB b2704 0.87 +0.6
2,825,734 + +0.0
2,825,807 + +0.3
2,825,921 - srlD b2705 0.21 +0.6
2,825,949 - srlD b2705 0.24 +0.7
2,825,949 - srlD b2705 0.24 -0.5
2,825,973 + srlD b2705 0.27 +0.3
2,826,044 - srlD b2705 0.37 +0.3
2,826,210 + srlD b2705 0.58 +1.4
2,826,210 + srlD b2705 0.58 -1.4
2,826,215 + srlD b2705 0.58 -0.2
2,826,218 - srlD b2705 0.59 +0.9
2,826,218 - srlD b2705 0.59 +0.3
2,826,245 + srlD b2705 0.62 +0.5
2,826,318 - srlD b2705 0.72 -0.3
2,826,397 + srlD b2705 0.82 -2.2
2,826,397 + srlD b2705 0.82 +0.1
2,826,397 + srlD b2705 0.82 -0.7
2,826,397 + srlD b2705 0.82 +0.3
2,826,474 + -0.4
2,826,474 + -0.1
2,826,482 - +0.5
2,826,490 - -0.5
2,826,490 - -0.2
2,826,613 - +0.4
2,826,613 - -0.2
2,826,613 - -0.4
2,826,646 - -0.5

Or see this region's nucleotide sequence