Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadL and yfdF are separated by 365 nucleotides yfdF and vacJ are separated by 181 nucleotides
b2344: fadL - long-chain fatty acid outer membrane transporter (RefSeq), at 2,459,328 to 2,460,668
fadL
b2345: yfdF - hypothetical protein (NCBI), at 2,461,034 to 2,462,092
yfdF
b2346: vacJ - predicted lipoprotein (NCBI), at 2,462,274 to 2,463,029
vacJ
Position (kb)
2461
2462
2463 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2460.075 kb on - strand, within fadL at 2460.075 kb on - strand, within fadL at 2460.124 kb on - strand, within fadL at 2460.124 kb on - strand, within fadL at 2460.289 kb on - strand, within fadL at 2460.289 kb on - strand, within fadL at 2460.352 kb on - strand, within fadL at 2460.397 kb on - strand, within fadL at 2460.399 kb on + strand, within fadL at 2460.399 kb on + strand, within fadL at 2460.399 kb on - strand, within fadL at 2460.413 kb on + strand, within fadL at 2460.421 kb on - strand, within fadL at 2460.433 kb on + strand, within fadL at 2460.440 kb on - strand, within fadL at 2460.467 kb on + strand, within fadL at 2460.467 kb on + strand, within fadL at 2460.483 kb on + strand, within fadL at 2460.484 kb on + strand, within fadL at 2460.484 kb on + strand, within fadL at 2460.484 kb on + strand, within fadL at 2460.487 kb on - strand, within fadL at 2460.516 kb on + strand, within fadL at 2460.521 kb on + strand, within fadL at 2460.521 kb on + strand, within fadL at 2460.579 kb on - strand at 2460.594 kb on + strand at 2460.600 kb on + strand at 2460.601 kb on + strand at 2460.601 kb on + strand at 2460.613 kb on - strand at 2460.646 kb on - strand at 2460.646 kb on - strand at 2460.646 kb on - strand at 2460.648 kb on + strand at 2460.673 kb on + strand at 2460.687 kb on + strand at 2460.691 kb on - strand at 2460.703 kb on - strand at 2460.703 kb on - strand at 2460.705 kb on - strand at 2460.706 kb on - strand at 2460.706 kb on - strand at 2460.713 kb on + strand at 2460.718 kb on - strand at 2460.724 kb on - strand at 2460.772 kb on - strand at 2460.810 kb on + strand at 2460.815 kb on - strand at 2460.850 kb on - strand at 2460.865 kb on + strand at 2460.865 kb on + strand at 2460.865 kb on + strand at 2460.899 kb on + strand at 2460.899 kb on + strand at 2460.907 kb on + strand at 2460.908 kb on + strand at 2460.908 kb on + strand at 2460.908 kb on + strand at 2460.908 kb on + strand at 2460.909 kb on + strand at 2460.913 kb on + strand at 2460.913 kb on + strand at 2460.915 kb on + strand at 2460.916 kb on - strand at 2460.917 kb on + strand at 2460.917 kb on + strand at 2460.917 kb on + strand at 2460.920 kb on + strand at 2460.920 kb on + strand at 2460.925 kb on + strand at 2461.077 kb on - strand at 2462.184 kb on - strand at 2462.208 kb on - strand at 2462.252 kb on - strand at 2462.309 kb on - strand at 2462.309 kb on - strand at 2462.309 kb on - strand at 2462.309 kb on - strand at 2462.333 kb on - strand at 2462.336 kb on - strand at 2462.344 kb on - strand at 2462.344 kb on - strand at 2462.394 kb on - strand, within vacJ at 2462.399 kb on - strand, within vacJ at 2462.403 kb on - strand, within vacJ at 2462.405 kb on - strand, within vacJ at 2462.406 kb on - strand, within vacJ at 2462.421 kb on - strand, within vacJ at 2462.438 kb on - strand, within vacJ at 2462.447 kb on + strand, within vacJ at 2462.465 kb on + strand, within vacJ at 2462.471 kb on - strand, within vacJ at 2462.491 kb on - strand, within vacJ at 2462.507 kb on - strand, within vacJ at 2462.619 kb on + strand, within vacJ at 2462.619 kb on + strand, within vacJ at 2462.623 kb on + strand, within vacJ at 2462.708 kb on - strand, within vacJ at 2462.799 kb on - strand, within vacJ at 2462.878 kb on + strand, within vacJ at 2462.909 kb on + strand, within vacJ at 2462.913 kb on - strand, within vacJ at 2463.052 kb on + strand at 2463.052 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 2,460,075 - fadL b2344 0.56 +1.5 2,460,075 - fadL b2344 0.56 +2.1 2,460,124 - fadL b2344 0.59 +1.6 2,460,124 - fadL b2344 0.59 +0.3 2,460,289 - fadL b2344 0.72 +1.0 2,460,289 - fadL b2344 0.72 +1.3 2,460,352 - fadL b2344 0.76 +1.4 2,460,397 - fadL b2344 0.80 +1.4 2,460,399 + fadL b2344 0.80 +1.5 2,460,399 + fadL b2344 0.80 +0.6 2,460,399 - fadL b2344 0.80 +1.8 2,460,413 + fadL b2344 0.81 +0.5 2,460,421 - fadL b2344 0.82 +0.6 2,460,433 + fadL b2344 0.82 +1.0 2,460,440 - fadL b2344 0.83 +1.0 2,460,467 + fadL b2344 0.85 +1.9 2,460,467 + fadL b2344 0.85 +0.6 2,460,483 + fadL b2344 0.86 +1.1 2,460,484 + fadL b2344 0.86 +1.1 2,460,484 + fadL b2344 0.86 +2.2 2,460,484 + fadL b2344 0.86 +1.3 2,460,487 - fadL b2344 0.86 +1.0 2,460,516 + fadL b2344 0.89 +1.0 2,460,521 + fadL b2344 0.89 +1.3 2,460,521 + fadL b2344 0.89 +1.3 2,460,579 - +1.4 2,460,594 + +1.3 2,460,600 + +1.2 2,460,601 + +1.4 2,460,601 + +1.0 2,460,613 - +2.1 2,460,646 - +1.6 2,460,646 - +1.5 2,460,646 - +1.5 2,460,648 + +0.7 2,460,673 + +0.1 2,460,687 + +0.2 2,460,691 - +0.3 2,460,703 - +0.8 2,460,703 - +1.3 2,460,705 - +0.6 2,460,706 - +1.3 2,460,706 - +1.0 2,460,713 + -0.7 2,460,718 - -0.7 2,460,724 - -1.1 2,460,772 - +0.5 2,460,810 + -0.0 2,460,815 - +0.0 2,460,850 - +0.0 2,460,865 + +0.3 2,460,865 + +0.1 2,460,865 + +0.6 2,460,899 + +0.1 2,460,899 + -1.5 2,460,907 + +0.1 2,460,908 + +0.2 2,460,908 + +1.2 2,460,908 + -0.3 2,460,908 + +0.1 2,460,909 + +0.6 2,460,913 + -1.1 2,460,913 + -0.3 2,460,915 + -0.9 2,460,916 - +0.4 2,460,917 + -0.0 2,460,917 + +0.2 2,460,917 + -0.8 2,460,920 + +0.6 2,460,920 + -0.2 2,460,925 + -0.3 2,461,077 - +0.5 2,462,184 - -0.0 2,462,208 - -0.2 2,462,252 - -0.4 2,462,309 - -3.2 2,462,309 - -1.9 2,462,309 - -1.7 2,462,309 - -3.6 2,462,333 - -2.6 2,462,336 - -4.0 2,462,344 - -2.0 2,462,344 - -3.4 2,462,394 - vacJ b2346 0.16 -2.7 2,462,399 - vacJ b2346 0.17 -2.4 2,462,403 - vacJ b2346 0.17 -2.5 2,462,405 - vacJ b2346 0.17 -2.9 2,462,406 - vacJ b2346 0.17 -4.2 2,462,421 - vacJ b2346 0.19 -3.0 2,462,438 - vacJ b2346 0.22 -4.4 2,462,447 + vacJ b2346 0.23 -3.3 2,462,465 + vacJ b2346 0.25 -4.4 2,462,471 - vacJ b2346 0.26 -3.3 2,462,491 - vacJ b2346 0.29 -1.8 2,462,507 - vacJ b2346 0.31 -2.5 2,462,619 + vacJ b2346 0.46 -2.9 2,462,619 + vacJ b2346 0.46 -3.0 2,462,623 + vacJ b2346 0.46 -3.3 2,462,708 - vacJ b2346 0.57 -2.6 2,462,799 - vacJ b2346 0.69 -2.7 2,462,878 + vacJ b2346 0.80 -2.0 2,462,909 + vacJ b2346 0.84 -3.4 2,462,913 - vacJ b2346 0.85 -3.0 2,463,052 + -1.9 2,463,052 + -2.1
Or see this region's nucleotide sequence