Strain Fitness in Escherichia coli BW25113 around b2096

Experiment: 2,4-Dinitrophenol 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgatZ and gatY are separated by 28 nucleotidesgatY and b2097 are separated by 307 nucleotides b2095: gatZ - D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI), at 2,173,081 to 2,174,343 gatZ b2096: gatY - tagatose-bisphosphate aldolase 1 (VIMSS), at 2,174,372 to 2,175,226 gatY b2097: b2097 - orf, hypothetical protein (VIMSS), at 2,175,534 to 2,176,586 b2097 Position (kb) 2174 2175 2176Strain fitness (log2 ratio) -1 0 1 2 3at 2173.440 kb on - strand, within gatZat 2173.444 kb on - strand, within gatZat 2173.451 kb on + strand, within gatZat 2173.451 kb on + strand, within gatZat 2173.459 kb on - strand, within gatZat 2173.459 kb on - strand, within gatZat 2173.485 kb on - strand, within gatZat 2173.588 kb on - strand, within gatZat 2173.638 kb on - strand, within gatZat 2173.717 kb on - strand, within gatZat 2173.783 kb on + strand, within gatZat 2173.791 kb on - strand, within gatZat 2173.791 kb on - strand, within gatZat 2173.870 kb on - strand, within gatZat 2174.277 kb on + strandat 2174.277 kb on + strandat 2174.285 kb on - strandat 2174.285 kb on - strandat 2174.285 kb on - strandat 2174.371 kb on + strandat 2174.422 kb on - strandat 2174.422 kb on - strandat 2174.469 kb on + strand, within gatYat 2174.749 kb on + strand, within gatYat 2174.749 kb on + strand, within gatYat 2175.002 kb on + strand, within gatYat 2175.029 kb on + strand, within gatYat 2175.129 kb on + strand, within gatYat 2175.189 kb on + strandat 2175.226 kb on + strandat 2175.226 kb on + strandat 2175.371 kb on + strandat 2175.371 kb on + strandat 2175.375 kb on - strandat 2175.377 kb on - strandat 2175.387 kb on - strandat 2175.431 kb on - strandat 2175.431 kb on - strandat 2175.431 kb on - strandat 2175.518 kb on - strandat 2175.518 kb on - strandat 2175.530 kb on - strandat 2175.530 kb on - strandat 2175.731 kb on + strand, within b2097at 2175.742 kb on + strand, within b2097at 2175.975 kb on + strand, within b2097at 2175.975 kb on + strand, within b2097at 2176.124 kb on + strand, within b2097at 2176.124 kb on + strand, within b2097at 2176.175 kb on - strand, within b2097

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Per-strain Table

Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM
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2,173,440 - gatZ b2095 0.28 +0.9
2,173,444 - gatZ b2095 0.29 +1.3
2,173,451 + gatZ b2095 0.29 +2.9
2,173,451 + gatZ b2095 0.29 +2.7
2,173,459 - gatZ b2095 0.30 +2.1
2,173,459 - gatZ b2095 0.30 +1.6
2,173,485 - gatZ b2095 0.32 +1.0
2,173,588 - gatZ b2095 0.40 +1.0
2,173,638 - gatZ b2095 0.44 +0.0
2,173,717 - gatZ b2095 0.50 +0.5
2,173,783 + gatZ b2095 0.56 +2.9
2,173,791 - gatZ b2095 0.56 +1.9
2,173,791 - gatZ b2095 0.56 +0.9
2,173,870 - gatZ b2095 0.62 +1.4
2,174,277 + +2.5
2,174,277 + +3.2
2,174,285 - +2.4
2,174,285 - +2.8
2,174,285 - +2.1
2,174,371 + +2.5
2,174,422 - -0.6
2,174,422 - -0.3
2,174,469 + gatY b2096 0.11 +2.0
2,174,749 + gatY b2096 0.44 +2.7
2,174,749 + gatY b2096 0.44 +2.7
2,175,002 + gatY b2096 0.74 +3.0
2,175,029 + gatY b2096 0.77 +2.4
2,175,129 + gatY b2096 0.89 +3.1
2,175,189 + +2.8
2,175,226 + +2.9
2,175,226 + +2.7
2,175,371 + +1.7
2,175,371 + +0.8
2,175,375 - -0.0
2,175,377 - +1.1
2,175,387 - +1.0
2,175,431 - +0.6
2,175,431 - +0.6
2,175,431 - +0.2
2,175,518 - +0.0
2,175,518 - +0.4
2,175,530 - -0.2
2,175,530 - +0.2
2,175,731 + b2097 b2097 0.19 -0.7
2,175,742 + b2097 b2097 0.20 -0.0
2,175,975 + b2097 b2097 0.42 +0.5
2,175,975 + b2097 b2097 0.42 +0.4
2,176,124 + b2097 b2097 0.56 -0.1
2,176,124 + b2097 b2097 0.56 -0.9
2,176,175 - b2097 b2097 0.61 +0.4

Or see this region's nucleotide sequence