Strain Fitness in Escherichia coli BW25113 around b1764

Experiment: 2,4-Dinitrophenol 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttopB and selD are separated by 4 nucleotidesselD and ydjA are separated by 116 nucleotidesydjA and sppA are separated by 160 nucleotides b1763: topB - DNA topoisomerase III (NCBI), at 1,843,023 to 1,844,984 topB b1764: selD - selenophosphate synthetase (NCBI), at 1,844,989 to 1,846,032 selD b1765: ydjA - predicted oxidoreductase (NCBI), at 1,846,149 to 1,846,700 ydjA b1766: sppA - protease IV (signal peptide peptidase) (NCBI), at 1,846,861 to 1,848,717 sppA Position (kb) 1844 1845 1846 1847Strain fitness (log2 ratio) -2 -1 0 1 2at 1844.351 kb on + strand, within topBat 1844.438 kb on + strand, within topBat 1844.443 kb on + strand, within topBat 1844.600 kb on - strand, within topBat 1844.829 kb on - strandat 1845.230 kb on - strand, within selDat 1845.242 kb on - strand, within selDat 1845.254 kb on - strand, within selDat 1845.300 kb on - strand, within selDat 1845.827 kb on - strand, within selDat 1845.827 kb on - strand, within selDat 1845.858 kb on - strand, within selDat 1845.881 kb on - strand, within selDat 1845.883 kb on - strand, within selDat 1846.260 kb on + strand, within ydjAat 1846.671 kb on - strandat 1846.671 kb on - strandat 1846.826 kb on - strandat 1846.828 kb on + strandat 1846.843 kb on - strandat 1846.849 kb on - strandat 1846.849 kb on - strandat 1846.860 kb on + strandat 1846.860 kb on + strandat 1846.910 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM
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1,844,351 + topB b1763 0.68 -0.4
1,844,438 + topB b1763 0.72 +0.3
1,844,443 + topB b1763 0.72 +0.4
1,844,600 - topB b1763 0.80 +0.8
1,844,829 - +2.4
1,845,230 - selD b1764 0.23 -2.2
1,845,242 - selD b1764 0.24 -2.3
1,845,254 - selD b1764 0.25 -2.0
1,845,300 - selD b1764 0.30 -1.5
1,845,827 - selD b1764 0.80 -1.2
1,845,827 - selD b1764 0.80 +0.8
1,845,858 - selD b1764 0.83 -1.5
1,845,881 - selD b1764 0.85 -1.9
1,845,883 - selD b1764 0.86 -1.9
1,846,260 + ydjA b1765 0.20 -1.3
1,846,671 - -0.6
1,846,671 - +0.2
1,846,826 - -0.6
1,846,828 + -0.1
1,846,843 - -0.3
1,846,849 - -0.8
1,846,849 - -0.4
1,846,860 + +0.8
1,846,860 + +0.1
1,846,910 - -0.5

Or see this region's nucleotide sequence