Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mipB and moeB are separated by 75 nucleotides moeB and moeA overlap by 1 nucleotides moeA and iaaA are separated by 203 nucleotides
b0825: mipB - putative transaldolase (VIMSS), at 862,865 to 863,527
mipB
b0826: moeB - molybdopterin biosynthesis protein MoeB (NCBI), at 863,603 to 864,352
moeB
b0827: moeA - molybdopterin biosynthesis protein (NCBI), at 864,352 to 865,587
moeA
b0828: iaaA - L-asparaginase (NCBI), at 865,791 to 866,756
iaaA
Position (kb)
864
865
866 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 863.433 kb on + strand, within mipB at 863.554 kb on + strand at 863.625 kb on - strand at 863.673 kb on - strand at 863.881 kb on + strand, within moeB at 863.881 kb on + strand, within moeB at 863.980 kb on - strand, within moeB at 863.987 kb on + strand, within moeB at 864.031 kb on - strand, within moeB at 864.031 kb on - strand, within moeB at 864.040 kb on - strand, within moeB at 864.067 kb on + strand, within moeB at 864.071 kb on - strand, within moeB at 864.129 kb on + strand, within moeB at 864.167 kb on + strand, within moeB at 864.209 kb on - strand, within moeB at 864.209 kb on - strand, within moeB at 864.214 kb on - strand, within moeB at 864.271 kb on - strand, within moeB at 864.647 kb on + strand, within moeA at 864.770 kb on - strand, within moeA at 864.770 kb on - strand, within moeA at 864.814 kb on - strand, within moeA at 864.855 kb on + strand, within moeA at 864.945 kb on + strand, within moeA at 865.047 kb on + strand, within moeA at 865.064 kb on - strand, within moeA at 865.220 kb on + strand, within moeA at 865.243 kb on + strand, within moeA at 865.243 kb on + strand, within moeA at 865.278 kb on - strand, within moeA at 865.278 kb on - strand at 865.301 kb on - strand, within moeA at 865.527 kb on + strand at 865.527 kb on + strand at 865.535 kb on - strand at 865.561 kb on - strand at 865.646 kb on + strand at 865.646 kb on + strand at 865.648 kb on + strand at 865.771 kb on + strand at 865.771 kb on + strand at 865.875 kb on + strand at 865.883 kb on + strand at 866.064 kb on - strand, within iaaA at 866.149 kb on + strand, within iaaA at 866.246 kb on + strand at 866.424 kb on + strand, within iaaA at 866.432 kb on - strand, within iaaA at 866.537 kb on + strand, within iaaA
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 863,433 + mipB b0825 0.86 -0.9 863,554 + -0.6 863,625 - +0.0 863,673 - -2.1 863,881 + moeB b0826 0.37 -0.1 863,881 + moeB b0826 0.37 -0.9 863,980 - moeB b0826 0.50 -1.1 863,987 + moeB b0826 0.51 -1.2 864,031 - moeB b0826 0.57 -1.0 864,031 - moeB b0826 0.57 -1.4 864,040 - moeB b0826 0.58 +0.5 864,067 + moeB b0826 0.62 -0.5 864,071 - moeB b0826 0.62 -2.0 864,129 + moeB b0826 0.70 -3.0 864,167 + moeB b0826 0.75 -2.3 864,209 - moeB b0826 0.81 -1.8 864,209 - moeB b0826 0.81 -0.5 864,214 - moeB b0826 0.81 -1.4 864,271 - moeB b0826 0.89 -1.4 864,647 + moeA b0827 0.24 -0.9 864,770 - moeA b0827 0.34 -1.0 864,770 - moeA b0827 0.34 -1.5 864,814 - moeA b0827 0.37 -1.2 864,855 + moeA b0827 0.41 -2.4 864,945 + moeA b0827 0.48 +0.0 865,047 + moeA b0827 0.56 -1.1 865,064 - moeA b0827 0.58 -2.7 865,220 + moeA b0827 0.70 -0.5 865,243 + moeA b0827 0.72 -2.5 865,243 + moeA b0827 0.72 -2.3 865,278 - moeA b0827 0.75 -1.3 865,278 - -0.1 865,301 - moeA b0827 0.77 -1.8 865,527 + -1.4 865,527 + -0.6 865,535 - -1.9 865,561 - -2.3 865,646 + +0.6 865,646 + +0.5 865,648 + +0.1 865,771 + -0.6 865,771 + -1.7 865,875 + +0.2 865,883 + +0.1 866,064 - iaaA b0828 0.28 -0.9 866,149 + iaaA b0828 0.37 -1.0 866,246 + +0.5 866,424 + iaaA b0828 0.66 -0.0 866,432 - iaaA b0828 0.66 -0.3 866,537 + iaaA b0828 0.77 -0.4
Or see this region's nucleotide sequence