Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepD and gpt are separated by 260 nucleotides gpt and frsA are separated by 91 nucleotides
b0237: pepD - aminoacyl-histidine dipeptidase (peptidase D) (NCBI), at 254,259 to 255,716
pepD
b0238: gpt - xanthine phosphoribosyltransferase (NCBI), at 255,977 to 256,435
gpt
b0239: frsA - fermentation/respiration switch protein (NCBI), at 256,527 to 257,771
frsA
Position (kb) 255
256
257 Strain fitness (log2 ratio) -2
-1
0
1 at 255.011 kb on + strand, within pepD at 255.011 kb on + strand, within pepD at 255.037 kb on + strand, within pepD at 255.042 kb on + strand, within pepD at 255.050 kb on - strand, within pepD at 255.128 kb on - strand, within pepD at 255.171 kb on - strand, within pepD at 255.206 kb on - strand, within pepD at 255.206 kb on - strand, within pepD at 255.246 kb on + strand, within pepD at 255.267 kb on + strand, within pepD at 255.267 kb on + strand, within pepD at 255.275 kb on - strand, within pepD at 255.355 kb on - strand, within pepD at 255.371 kb on + strand, within pepD at 255.396 kb on + strand, within pepD at 255.406 kb on - strand, within pepD at 255.426 kb on - strand, within pepD at 255.426 kb on - strand, within pepD at 255.428 kb on + strand, within pepD at 255.428 kb on + strand, within pepD at 255.447 kb on + strand, within pepD at 255.450 kb on + strand, within pepD at 255.455 kb on - strand, within pepD at 255.527 kb on - strand, within pepD at 255.715 kb on - strand at 255.791 kb on + strand at 255.826 kb on - strand at 255.841 kb on - strand at 255.841 kb on - strand at 255.902 kb on + strand at 255.912 kb on - strand at 255.912 kb on - strand at 255.971 kb on + strand at 256.050 kb on + strand, within gpt at 256.050 kb on + strand, within gpt at 256.050 kb on + strand, within gpt at 256.092 kb on + strand, within gpt at 256.250 kb on - strand, within gpt at 256.250 kb on - strand, within gpt at 256.401 kb on - strand at 256.429 kb on - strand at 256.429 kb on - strand at 256.479 kb on - strand at 256.491 kb on + strand at 256.510 kb on + strand at 256.554 kb on - strand at 256.603 kb on + strand at 256.621 kb on - strand at 256.621 kb on - strand at 256.675 kb on + strand, within frsA at 256.705 kb on + strand, within frsA at 256.705 kb on + strand, within frsA at 256.749 kb on - strand, within frsA at 256.764 kb on - strand, within frsA at 256.791 kb on + strand, within frsA at 256.806 kb on + strand, within frsA at 256.818 kb on + strand, within frsA at 256.836 kb on - strand, within frsA at 256.836 kb on - strand, within frsA at 256.864 kb on + strand, within frsA at 256.884 kb on - strand, within frsA at 257.061 kb on + strand, within frsA at 257.061 kb on + strand, within frsA at 257.115 kb on + strand, within frsA at 257.121 kb on - strand, within frsA at 257.121 kb on - strand, within frsA at 257.159 kb on - strand, within frsA at 257.161 kb on - strand, within frsA at 257.358 kb on - strand, within frsA at 257.432 kb on - strand, within frsA
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 255,011 + pepD b0237 0.52 -1.1 255,011 + pepD b0237 0.52 -0.3 255,037 + pepD b0237 0.53 -0.9 255,042 + pepD b0237 0.54 -1.0 255,050 - pepD b0237 0.54 -0.1 255,128 - pepD b0237 0.60 -0.4 255,171 - pepD b0237 0.63 -0.3 255,206 - pepD b0237 0.65 +0.0 255,206 - pepD b0237 0.65 -1.5 255,246 + pepD b0237 0.68 -1.0 255,267 + pepD b0237 0.69 -1.6 255,267 + pepD b0237 0.69 -0.0 255,275 - pepD b0237 0.70 -0.1 255,355 - pepD b0237 0.75 -1.0 255,371 + pepD b0237 0.76 -0.3 255,396 + pepD b0237 0.78 +0.6 255,406 - pepD b0237 0.79 -0.5 255,426 - pepD b0237 0.80 -1.8 255,426 - pepD b0237 0.80 -0.9 255,428 + pepD b0237 0.80 -0.3 255,428 + pepD b0237 0.80 -1.2 255,447 + pepD b0237 0.81 -1.3 255,450 + pepD b0237 0.82 +0.1 255,455 - pepD b0237 0.82 -0.3 255,527 - pepD b0237 0.87 -0.9 255,715 - -1.4 255,791 + -0.5 255,826 - +0.1 255,841 - +0.6 255,841 - +0.9 255,902 + -0.6 255,912 - +1.0 255,912 - +1.0 255,971 + +1.3 256,050 + gpt b0238 0.16 -0.9 256,050 + gpt b0238 0.16 +0.1 256,050 + gpt b0238 0.16 -1.5 256,092 + gpt b0238 0.25 +0.3 256,250 - gpt b0238 0.59 +0.0 256,250 - gpt b0238 0.59 -0.7 256,401 - +0.5 256,429 - -1.1 256,429 - +0.5 256,479 - -1.1 256,491 + +0.2 256,510 + +0.6 256,554 - +1.0 256,603 + +0.4 256,621 - +1.3 256,621 - +0.2 256,675 + frsA b0239 0.12 +0.2 256,705 + frsA b0239 0.14 -0.3 256,705 + frsA b0239 0.14 +0.3 256,749 - frsA b0239 0.18 +0.8 256,764 - frsA b0239 0.19 +0.8 256,791 + frsA b0239 0.21 +0.1 256,806 + frsA b0239 0.22 -0.4 256,818 + frsA b0239 0.23 -0.1 256,836 - frsA b0239 0.25 -0.6 256,836 - frsA b0239 0.25 +0.1 256,864 + frsA b0239 0.27 +0.4 256,884 - frsA b0239 0.29 +0.1 257,061 + frsA b0239 0.43 -0.6 257,061 + frsA b0239 0.43 -0.4 257,115 + frsA b0239 0.47 +0.9 257,121 - frsA b0239 0.48 +0.3 257,121 - frsA b0239 0.48 +0.5 257,159 - frsA b0239 0.51 +0.1 257,161 - frsA b0239 0.51 +0.1 257,358 - frsA b0239 0.67 +0.4 257,432 - frsA b0239 0.73 +0.2
Or see this region's nucleotide sequence