Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhuA and fhuC are separated by 50 nucleotides fhuC and fhuD overlap by 1 nucleotides fhuD and fhuB overlap by 4 nucleotides
b0150: fhuA - ferrichrome outer membrane transporter (NCBI), at 167,484 to 169,727
fhuA
b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575
fhuC
b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465
fhuD
b0153: fhuB - fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components (NCBI), at 171,462 to 173,444
fhuB
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1 at 168.800 kb on + strand, within fhuA at 168.899 kb on + strand, within fhuA at 168.942 kb on - strand, within fhuA at 168.944 kb on - strand, within fhuA at 168.944 kb on - strand, within fhuA at 168.961 kb on + strand, within fhuA at 169.064 kb on + strand, within fhuA at 169.127 kb on + strand, within fhuA at 169.127 kb on + strand, within fhuA at 169.135 kb on - strand, within fhuA at 169.135 kb on - strand, within fhuA at 169.167 kb on - strand, within fhuA at 169.167 kb on - strand, within fhuA at 169.202 kb on + strand, within fhuA at 169.260 kb on + strand, within fhuA at 169.330 kb on + strand, within fhuA at 169.354 kb on - strand, within fhuA at 169.354 kb on - strand, within fhuA at 169.355 kb on - strand, within fhuA at 169.361 kb on + strand, within fhuA at 169.390 kb on - strand, within fhuA at 169.437 kb on + strand, within fhuA at 169.437 kb on + strand, within fhuA at 169.467 kb on + strand, within fhuA at 169.483 kb on + strand, within fhuA at 169.490 kb on + strand, within fhuA at 169.528 kb on + strand at 169.549 kb on - strand at 169.556 kb on - strand at 169.594 kb on - strand at 169.620 kb on + strand at 169.629 kb on - strand at 169.635 kb on - strand at 169.635 kb on - strand at 169.639 kb on - strand at 169.684 kb on - strand at 169.827 kb on - strand at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.929 kb on - strand, within fhuC at 169.965 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.989 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.250 kb on + strand, within fhuC at 170.250 kb on - strand, within fhuC at 170.255 kb on + strand, within fhuC at 170.257 kb on + strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.395 kb on - strand, within fhuC at 170.654 kb on - strand at 170.671 kb on + strand, within fhuD at 170.671 kb on + strand, within fhuD at 170.689 kb on - strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.775 kb on + strand, within fhuD at 170.831 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.972 kb on - strand, within fhuD at 171.065 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.219 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.307 kb on - strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.423 kb on + strand at 171.423 kb on - strand at 171.423 kb on - strand at 171.506 kb on + strand at 171.514 kb on - strand at 171.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 168,800 + fhuA b0150 0.59 +0.8 168,899 + fhuA b0150 0.63 +0.4 168,942 - fhuA b0150 0.65 +0.3 168,944 - fhuA b0150 0.65 +0.5 168,944 - fhuA b0150 0.65 -0.6 168,961 + fhuA b0150 0.66 +0.0 169,064 + fhuA b0150 0.70 +0.4 169,127 + fhuA b0150 0.73 +0.2 169,127 + fhuA b0150 0.73 +0.5 169,135 - fhuA b0150 0.74 -0.1 169,135 - fhuA b0150 0.74 +0.6 169,167 - fhuA b0150 0.75 +0.4 169,167 - fhuA b0150 0.75 -0.4 169,202 + fhuA b0150 0.77 -0.3 169,260 + fhuA b0150 0.79 -0.4 169,330 + fhuA b0150 0.82 -0.8 169,354 - fhuA b0150 0.83 +0.3 169,354 - fhuA b0150 0.83 +0.1 169,355 - fhuA b0150 0.83 +0.1 169,361 + fhuA b0150 0.84 -0.4 169,390 - fhuA b0150 0.85 +0.6 169,437 + fhuA b0150 0.87 -0.7 169,437 + fhuA b0150 0.87 -0.7 169,467 + fhuA b0150 0.88 -0.4 169,483 + fhuA b0150 0.89 -0.8 169,490 + fhuA b0150 0.89 -0.6 169,528 + -0.6 169,549 - +0.2 169,556 - +0.3 169,594 - -2.2 169,620 + +0.0 169,629 - -0.0 169,635 - +0.1 169,635 - +1.5 169,639 - -0.1 169,684 - -0.0 169,827 - +0.3 169,926 + fhuC b0151 0.19 -0.9 169,926 + fhuC b0151 0.19 -1.2 169,926 + fhuC b0151 0.19 -1.7 169,929 - fhuC b0151 0.19 -0.3 169,965 + fhuC b0151 0.23 -0.6 169,966 + fhuC b0151 0.24 +0.1 169,966 + fhuC b0151 0.24 -0.3 169,989 + fhuC b0151 0.26 -0.8 170,210 + fhuC b0151 0.54 +0.2 170,210 + fhuC b0151 0.54 -1.4 170,250 + fhuC b0151 0.59 -0.6 170,250 - fhuC b0151 0.59 -0.6 170,255 + fhuC b0151 0.60 -0.9 170,257 + fhuC b0151 0.60 -0.4 170,390 - fhuC b0151 0.77 +0.3 170,390 - fhuC b0151 0.77 -0.0 170,395 - fhuC b0151 0.77 -1.0 170,654 - +0.1 170,671 + fhuD b0152 0.11 -0.5 170,671 + fhuD b0152 0.11 -0.8 170,689 - fhuD b0152 0.13 -1.0 170,739 + fhuD b0152 0.18 -0.2 170,739 + fhuD b0152 0.18 +0.1 170,755 - fhuD b0152 0.20 -2.3 170,755 - fhuD b0152 0.20 +0.1 170,775 + fhuD b0152 0.22 -1.1 170,831 + fhuD b0152 0.29 -1.0 170,869 + fhuD b0152 0.33 -0.6 170,869 + fhuD b0152 0.33 -1.3 170,895 - fhuD b0152 0.36 +0.6 170,895 - fhuD b0152 0.36 -0.1 170,972 - fhuD b0152 0.45 -0.3 171,065 + fhuD b0152 0.55 -1.4 171,199 + fhuD b0152 0.70 -1.8 171,199 + fhuD b0152 0.70 +0.2 171,219 + fhuD b0152 0.72 -0.6 171,225 + fhuD b0152 0.73 +0.9 171,225 + fhuD b0152 0.73 +0.2 171,228 + fhuD b0152 0.73 -0.7 171,228 + fhuD b0152 0.73 +0.5 171,238 - fhuD b0152 0.74 -1.4 171,238 - fhuD b0152 0.74 -1.1 171,307 - fhuD b0152 0.82 -1.3 171,336 + fhuD b0152 0.85 +0.1 171,336 + fhuD b0152 0.85 -0.2 171,423 + -1.0 171,423 - -0.0 171,423 - +0.2 171,506 + -0.5 171,514 - -0.7 171,533 - -0.5
Or see this region's nucleotide sequence