Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mraZ and mraW are separated by 1 nucleotides mraW and ftsL overlap by 4 nucleotides ftsL and ftsI are separated by 15 nucleotides
b0081: mraZ - hypothetical protein (NCBI), at 89,634 to 90,092
mraZ
b0082: mraW - S-adenosyl-methyltransferase (NCBI), at 90,094 to 91,035
mraW
b0083: ftsL - membrane bound cell division protein at septum containing leucine zipper motif (NCBI), at 91,032 to 91,397
ftsL
b0084: ftsI - transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3) (NCBI), at 91,413 to 93,179
ftsI
Position (kb) 91
92 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 90.039 kb on + strand, within mraZ at 90.039 kb on + strand, within mraZ at 90.042 kb on + strand, within mraZ at 90.042 kb on + strand, within mraZ at 90.230 kb on + strand, within mraW at 90.473 kb on + strand, within mraW at 90.518 kb on + strand, within mraW at 90.544 kb on + strand, within mraW at 90.681 kb on + strand, within mraW at 90.701 kb on + strand, within mraW at 90.783 kb on + strand, within mraW at 90.887 kb on + strand, within mraW at 90.896 kb on + strand, within mraW at 90.899 kb on + strand, within mraW at 90.979 kb on + strand at 91.008 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.028 kb on + strand at 91.028 kb on + strand at 91.357 kb on + strand at 91.362 kb on + strand at 91.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 90,039 + mraZ b0081 0.88 +0.3 90,039 + mraZ b0081 0.88 -0.4 90,042 + mraZ b0081 0.89 -0.4 90,042 + mraZ b0081 0.89 -1.2 90,230 + mraW b0082 0.14 -2.2 90,473 + mraW b0082 0.40 -2.3 90,518 + mraW b0082 0.45 -3.3 90,544 + mraW b0082 0.48 -2.3 90,681 + mraW b0082 0.62 -2.9 90,701 + mraW b0082 0.64 -2.3 90,783 + mraW b0082 0.73 -1.4 90,887 + mraW b0082 0.84 -3.1 90,896 + mraW b0082 0.85 -2.4 90,899 + mraW b0082 0.85 -0.6 90,979 + -1.9 91,008 + -1.4 91,020 + -0.6 91,020 + -0.8 91,020 + -1.3 91,028 + -2.6 91,028 + -0.4 91,357 + +1.1 91,362 + -2.1 91,378 + -1.0
Or see this region's nucleotide sequence