Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruR and mraZ are separated by 601 nucleotides mraZ and mraW are separated by 1 nucleotides mraW and ftsL overlap by 4 nucleotides
b0080: fruR - DNA-binding transcriptional dual regulator (NCBI), at 88,028 to 89,032
fruR
b0081: mraZ - hypothetical protein (NCBI), at 89,634 to 90,092
mraZ
b0082: mraW - S-adenosyl-methyltransferase (NCBI), at 90,094 to 91,035
mraW
b0083: ftsL - membrane bound cell division protein at septum containing leucine zipper motif (NCBI), at 91,032 to 91,397
ftsL
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 88.683 kb on + strand, within fruR at 88.753 kb on + strand, within fruR at 88.772 kb on + strand at 88.774 kb on + strand, within fruR at 88.821 kb on + strand, within fruR at 88.851 kb on + strand, within fruR at 88.851 kb on + strand, within fruR at 88.868 kb on + strand, within fruR at 88.894 kb on + strand, within fruR at 88.937 kb on + strand at 88.945 kb on - strand at 88.945 kb on - strand at 88.946 kb on + strand at 88.949 kb on - strand at 88.992 kb on + strand at 89.000 kb on + strand at 89.001 kb on - strand at 89.012 kb on + strand at 89.015 kb on + strand at 89.015 kb on + strand at 89.017 kb on + strand at 89.022 kb on + strand at 89.023 kb on - strand at 89.023 kb on - strand at 89.023 kb on - strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.086 kb on + strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.091 kb on + strand at 89.122 kb on + strand at 89.132 kb on + strand at 89.225 kb on + strand at 89.294 kb on - strand at 89.307 kb on + strand at 89.307 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.313 kb on - strand at 89.315 kb on - strand at 89.325 kb on + strand at 89.325 kb on - strand at 89.330 kb on - strand at 89.391 kb on + strand at 89.393 kb on + strand at 89.401 kb on - strand at 89.411 kb on + strand at 89.411 kb on + strand at 89.416 kb on - strand at 89.508 kb on + strand at 89.508 kb on + strand at 89.542 kb on - strand at 89.542 kb on - strand at 89.550 kb on + strand at 89.558 kb on - strand at 89.649 kb on + strand at 89.649 kb on + strand at 89.658 kb on + strand at 89.680 kb on + strand, within mraZ at 89.685 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.981 kb on + strand, within mraZ at 90.039 kb on + strand, within mraZ at 90.039 kb on + strand, within mraZ at 90.042 kb on + strand, within mraZ at 90.042 kb on + strand, within mraZ at 90.230 kb on + strand, within mraW at 90.473 kb on + strand, within mraW at 90.518 kb on + strand, within mraW at 90.544 kb on + strand, within mraW at 90.681 kb on + strand, within mraW at 90.701 kb on + strand, within mraW at 90.783 kb on + strand, within mraW at 90.887 kb on + strand, within mraW at 90.896 kb on + strand, within mraW at 90.899 kb on + strand, within mraW at 90.979 kb on + strand at 91.008 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.028 kb on + strand at 91.028 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 88,683 + fruR b0080 0.65 -2.6 88,753 + fruR b0080 0.72 -1.9 88,772 + +0.3 88,774 + fruR b0080 0.74 -1.4 88,821 + fruR b0080 0.79 -0.8 88,851 + fruR b0080 0.82 -0.6 88,851 + fruR b0080 0.82 -2.3 88,868 + fruR b0080 0.84 -2.4 88,894 + fruR b0080 0.86 -2.7 88,937 + -2.0 88,945 - -0.5 88,945 - -0.1 88,946 + -1.2 88,949 - -1.7 88,992 + -1.9 89,000 + -1.9 89,001 - -0.4 89,012 + -1.8 89,015 + -0.3 89,015 + -1.3 89,017 + -0.0 89,022 + -0.0 89,023 - +0.2 89,023 - -1.9 89,023 - -1.5 89,079 + -0.1 89,079 + +0.1 89,079 + +0.5 89,079 + +0.7 89,079 + -0.9 89,079 + +0.7 89,086 + -0.8 89,087 - +0.4 89,087 - +1.1 89,087 - +0.8 89,087 - +0.9 89,087 - +0.4 89,087 - +1.1 89,089 - +0.0 89,089 - +1.2 89,089 - +1.3 89,089 - +1.0 89,091 + +0.8 89,122 + +0.8 89,132 + -0.3 89,225 + +0.1 89,294 - -1.5 89,307 + -0.3 89,307 + -1.1 89,312 + -0.6 89,312 + -0.8 89,312 + -0.6 89,313 - +0.2 89,315 - -0.9 89,325 + -0.3 89,325 - -0.7 89,330 - -0.7 89,391 + -0.1 89,393 + -0.1 89,401 - -0.2 89,411 + -0.1 89,411 + -1.6 89,416 - -0.6 89,508 + -0.2 89,508 + +0.8 89,542 - +0.2 89,542 - -0.7 89,550 + -1.7 89,558 - +0.1 89,649 + -0.9 89,649 + -0.2 89,658 + +1.3 89,680 + mraZ b0081 0.10 -0.5 89,685 + mraZ b0081 0.11 -0.9 89,917 + mraZ b0081 0.62 -0.1 89,917 + mraZ b0081 0.62 -1.1 89,981 + mraZ b0081 0.76 -1.0 90,039 + mraZ b0081 0.88 +0.3 90,039 + mraZ b0081 0.88 -0.4 90,042 + mraZ b0081 0.89 -0.4 90,042 + mraZ b0081 0.89 -1.2 90,230 + mraW b0082 0.14 -2.2 90,473 + mraW b0082 0.40 -2.3 90,518 + mraW b0082 0.45 -3.3 90,544 + mraW b0082 0.48 -2.3 90,681 + mraW b0082 0.62 -2.9 90,701 + mraW b0082 0.64 -2.3 90,783 + mraW b0082 0.73 -1.4 90,887 + mraW b0082 0.84 -3.1 90,896 + mraW b0082 0.85 -2.4 90,899 + mraW b0082 0.85 -0.6 90,979 + -1.9 91,008 + -1.4 91,020 + -0.6 91,020 + -0.8 91,020 + -1.3 91,028 + -2.6 91,028 + -0.4
Or see this region's nucleotide sequence