Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaW and yaaI are separated by 25 nucleotides yaaI and dnaK are separated by 376 nucleotides dnaK and dnaJ are separated by 88 nucleotides
b0011: yaaW - hypothetical protein (NCBI), at 10,643 to 11,356
yaaW
b0013: yaaI - hypothetical protein (NCBI), at 11,382 to 11,786
yaaI
b0014: dnaK - molecular chaperone DnaK (NCBI), at 12,163 to 14,079
dnaK
b0015: dnaJ - chaperone Hsp40, co-chaperone with DnaK (NCBI), at 14,168 to 15,298
dnaJ
Position (kb) 12
13
14
15 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 11.196 kb on - strand, within yaaW at 11.196 kb on - strand, within yaaW at 11.235 kb on - strand, within yaaW at 11.235 kb on - strand, within yaaW at 11.304 kb on + strand at 11.398 kb on - strand at 11.398 kb on - strand at 11.399 kb on + strand at 11.399 kb on + strand at 11.410 kb on + strand at 11.416 kb on + strand at 11.422 kb on + strand at 11.432 kb on - strand, within yaaI at 11.506 kb on - strand, within yaaI at 11.506 kb on - strand, within yaaI at 11.541 kb on - strand, within yaaI at 11.546 kb on + strand, within yaaI at 11.546 kb on + strand, within yaaI at 11.601 kb on + strand, within yaaI at 11.613 kb on + strand, within yaaI at 11.614 kb on - strand, within yaaI at 11.651 kb on + strand, within yaaI at 11.718 kb on + strand, within yaaI at 11.718 kb on + strand, within yaaI at 12.025 kb on + strand at 12.057 kb on + strand at 12.141 kb on + strand at 13.673 kb on + strand, within dnaK at 13.673 kb on + strand, within dnaK at 13.673 kb on + strand, within dnaK at 13.859 kb on + strand, within dnaK at 13.894 kb on + strand at 13.894 kb on + strand at 14.002 kb on + strand at 14.116 kb on + strand at 14.145 kb on + strand at 14.245 kb on - strand at 14.260 kb on - strand at 14.264 kb on + strand at 14.264 kb on + strand at 14.325 kb on + strand, within dnaJ at 14.371 kb on + strand, within dnaJ at 14.374 kb on - strand, within dnaJ at 14.508 kb on + strand, within dnaJ at 14.508 kb on + strand, within dnaJ at 14.521 kb on + strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.596 kb on + strand, within dnaJ at 14.596 kb on + strand, within dnaJ at 14.596 kb on - strand, within dnaJ at 14.627 kb on - strand, within dnaJ at 14.664 kb on + strand, within dnaJ at 14.664 kb on + strand, within dnaJ at 14.672 kb on - strand, within dnaJ at 14.682 kb on - strand, within dnaJ at 14.702 kb on + strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.745 kb on + strand, within dnaJ at 14.781 kb on - strand, within dnaJ at 14.781 kb on - strand, within dnaJ at 14.829 kb on + strand, within dnaJ at 14.829 kb on + strand, within dnaJ at 14.842 kb on - strand, within dnaJ at 14.842 kb on - strand, within dnaJ at 14.859 kb on + strand, within dnaJ at 14.859 kb on + strand, within dnaJ at 14.923 kb on + strand, within dnaJ at 14.959 kb on - strand, within dnaJ
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 11,196 - yaaW b0011 0.77 +1.1 11,196 - yaaW b0011 0.77 -0.7 11,235 - yaaW b0011 0.83 -0.9 11,235 - yaaW b0011 0.83 -0.5 11,304 + -0.2 11,398 - -0.0 11,398 - +1.2 11,399 + -0.1 11,399 + +0.2 11,410 + -1.4 11,416 + -0.1 11,422 + -0.7 11,432 - yaaI b0013 0.12 -0.8 11,506 - yaaI b0013 0.31 -1.5 11,506 - yaaI b0013 0.31 +0.8 11,541 - yaaI b0013 0.39 +0.2 11,546 + yaaI b0013 0.40 +0.4 11,546 + yaaI b0013 0.40 -0.3 11,601 + yaaI b0013 0.54 -0.2 11,613 + yaaI b0013 0.57 +0.3 11,614 - yaaI b0013 0.57 -0.1 11,651 + yaaI b0013 0.66 -0.2 11,718 + yaaI b0013 0.83 +0.8 11,718 + yaaI b0013 0.83 -0.7 12,025 + -0.7 12,057 + -0.3 12,141 + -0.2 13,673 + dnaK b0014 0.79 -2.6 13,673 + dnaK b0014 0.79 -2.0 13,673 + dnaK b0014 0.79 -3.0 13,859 + dnaK b0014 0.88 -1.5 13,894 + -4.2 13,894 + -0.4 14,002 + -0.9 14,116 + -0.8 14,145 + -0.5 14,245 - -1.9 14,260 - -0.7 14,264 + -0.9 14,264 + -2.0 14,325 + dnaJ b0015 0.14 -3.2 14,371 + dnaJ b0015 0.18 -1.6 14,374 - dnaJ b0015 0.18 -2.9 14,508 + dnaJ b0015 0.30 -1.0 14,508 + dnaJ b0015 0.30 -1.7 14,521 + dnaJ b0015 0.31 -2.7 14,594 - dnaJ b0015 0.38 -2.8 14,594 - dnaJ b0015 0.38 -0.8 14,594 - dnaJ b0015 0.38 -3.4 14,596 + dnaJ b0015 0.38 +1.1 14,596 + dnaJ b0015 0.38 -0.1 14,596 - dnaJ b0015 0.38 -2.6 14,627 - dnaJ b0015 0.41 -1.6 14,664 + dnaJ b0015 0.44 -0.9 14,664 + dnaJ b0015 0.44 -2.1 14,672 - dnaJ b0015 0.45 -0.4 14,682 - dnaJ b0015 0.45 -2.1 14,702 + dnaJ b0015 0.47 -3.3 14,713 - dnaJ b0015 0.48 -2.0 14,713 - dnaJ b0015 0.48 -3.5 14,713 - dnaJ b0015 0.48 -3.8 14,745 + dnaJ b0015 0.51 -2.9 14,781 - dnaJ b0015 0.54 -3.6 14,781 - dnaJ b0015 0.54 -1.8 14,829 + dnaJ b0015 0.58 -0.9 14,829 + dnaJ b0015 0.58 -2.4 14,842 - dnaJ b0015 0.60 -1.9 14,842 - dnaJ b0015 0.60 -1.2 14,859 + dnaJ b0015 0.61 -1.7 14,859 + dnaJ b0015 0.61 -2.2 14,923 + dnaJ b0015 0.67 -0.9 14,959 - dnaJ b0015 0.70 -2.9
Or see this region's nucleotide sequence