Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF484 and GFF485 overlap by 4 nucleotides GFF485 and GFF486 overlap by 4 nucleotides GFF486 and GFF487 are separated by 32 nucleotides GFF487 and GFF488 are separated by 110 nucleotides GFF488 and GFF489 are separated by 0 nucleotides
GFF484 - FIG002337: predicted inner membrane protein, at 204,979 to 206,658
GFF484
GFF485 - FIG005119: putative inner membrane protein, at 206,655 to 206,846
GFF485
GFF486 - FIG005274: hypothetical protein, at 206,843 to 208,402
GFF486
GFF487 - hypothetical protein, at 208,435 to 208,548
GFF487
GFF488 - FIG004405: Putative cytoplasmic protein, at 208,659 to 208,862
GFF488
GFF489 - Cellulose synthase, putative, at 208,863 to 209,615
GFF489
Position (kb)
206
207
208
209 Strain fitness (log2 ratio)
-1
0
1 at 205.880 kb on - strand, within GFF484 at 205.935 kb on + strand, within GFF484 at 206.018 kb on + strand, within GFF484 at 206.019 kb on - strand, within GFF484 at 206.219 kb on - strand, within GFF484 at 206.263 kb on + strand, within GFF484 at 206.386 kb on - strand, within GFF484 at 206.486 kb on - strand, within GFF484 at 206.609 kb on + strand at 206.709 kb on + strand, within GFF485 at 206.710 kb on - strand, within GFF485 at 206.731 kb on - strand, within GFF485 at 206.745 kb on - strand, within GFF485 at 206.928 kb on - strand at 206.987 kb on + strand at 206.988 kb on - strand at 207.079 kb on + strand, within GFF486 at 207.157 kb on + strand, within GFF486 at 207.158 kb on - strand, within GFF486 at 207.186 kb on - strand, within GFF486 at 207.192 kb on - strand, within GFF486 at 207.283 kb on + strand, within GFF486 at 207.284 kb on - strand, within GFF486 at 207.413 kb on - strand, within GFF486 at 207.511 kb on + strand, within GFF486 at 207.604 kb on + strand, within GFF486 at 207.815 kb on + strand, within GFF486 at 208.049 kb on + strand, within GFF486 at 208.166 kb on - strand, within GFF486 at 208.203 kb on - strand, within GFF486 at 208.949 kb on - strand, within GFF489 at 209.080 kb on + strand, within GFF489 at 209.085 kb on + strand, within GFF489 at 209.086 kb on - strand, within GFF489 at 209.087 kb on + strand, within GFF489 at 209.087 kb on + strand, within GFF489 at 209.088 kb on - strand, within GFF489 at 209.216 kb on + strand, within GFF489 at 209.216 kb on + strand, within GFF489 at 209.217 kb on - strand, within GFF489 at 209.288 kb on + strand, within GFF489 at 209.398 kb on + strand, within GFF489
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 205,880 - GFF484 0.54 -0.3 205,935 + GFF484 0.57 -0.1 206,018 + GFF484 0.62 -0.2 206,019 - GFF484 0.62 +0.3 206,219 - GFF484 0.74 -0.1 206,263 + GFF484 0.76 -0.1 206,386 - GFF484 0.84 -0.3 206,486 - GFF484 0.90 -0.0 206,609 + -0.6 206,709 + GFF485 0.28 +0.9 206,710 - GFF485 0.29 +0.1 206,731 - GFF485 0.40 +0.6 206,745 - GFF485 0.47 +0.5 206,928 - +0.1 206,987 + +0.2 206,988 - -0.5 207,079 + GFF486 0.15 -0.5 207,157 + GFF486 0.20 +0.0 207,158 - GFF486 0.20 +0.3 207,186 - GFF486 0.22 +0.4 207,192 - GFF486 0.22 +0.1 207,283 + GFF486 0.28 -1.5 207,284 - GFF486 0.28 -0.4 207,413 - GFF486 0.37 -0.5 207,511 + GFF486 0.43 -0.2 207,604 + GFF486 0.49 -0.9 207,815 + GFF486 0.62 +0.0 208,049 + GFF486 0.77 -0.0 208,166 - GFF486 0.85 -0.2 208,203 - GFF486 0.87 -0.3 208,949 - GFF489 0.11 +0.0 209,080 + GFF489 0.29 -0.6 209,085 + GFF489 0.29 +0.1 209,086 - GFF489 0.30 -1.0 209,087 + GFF489 0.30 +0.2 209,087 + GFF489 0.30 +0.4 209,088 - GFF489 0.30 -0.2 209,216 + GFF489 0.47 +0.2 209,216 + GFF489 0.47 +0.5 209,217 - GFF489 0.47 +0.0 209,288 + GFF489 0.56 -1.2 209,398 + GFF489 0.71 -0.4
Or see this region's nucleotide sequence