Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4825 and GFF4826 are separated by 477 nucleotides GFF4826 and GFF4827 overlap by 14 nucleotides GFF4827 and GFF4828 overlap by 4 nucleotides GFF4828 and GFF4829 overlap by 1 nucleotides GFF4829 and GFF4830 are separated by 276 nucleotides GFF4830 and GFF4831 are separated by 30 nucleotides
GFF4825 - hypothetical protein, at 124,727 to 124,963
GFF4825
GFF4826 - FIG01046316: hypothetical protein, at 125,441 to 125,572
GFF4826
GFF4827 - FIG01047314: hypothetical protein, at 125,559 to 125,744
GFF4827
GFF4828 - FIG01047314: hypothetical protein, at 125,741 to 125,887
GFF4828
GFF4829 - hypothetical protein, at 125,887 to 126,069
GFF4829
GFF4830 - hypothetical protein, at 126,346 to 126,492
GFF4830
GFF4831 - Queuosine Biosynthesis QueE Radical SAM, at 126,523 to 127,194
GFF4831
Position (kb)
125
126 Strain fitness (log2 ratio)
-1
0
1 at 124.601 kb on + strand at 124.701 kb on + strand at 124.962 kb on - strand at 125.007 kb on + strand at 125.088 kb on - strand at 125.088 kb on - strand at 125.441 kb on - strand at 125.594 kb on - strand, within GFF4827 at 125.874 kb on - strand at 125.874 kb on - strand at 125.876 kb on - strand at 125.876 kb on - strand at 125.876 kb on - strand at 125.878 kb on - strand at 125.878 kb on - strand at 125.878 kb on - strand at 125.878 kb on - strand at 125.885 kb on + strand at 125.945 kb on + strand, within GFF4829 at 126.124 kb on + strand at 126.240 kb on - strand at 126.469 kb on + strand, within GFF4830 at 126.516 kb on + strand at 126.591 kb on + strand, within GFF4831 at 126.610 kb on + strand, within GFF4831 at 126.621 kb on + strand, within GFF4831
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 124,601 + -0.3 124,701 + +0.5 124,962 - +0.0 125,007 + -0.8 125,088 - -1.4 125,088 - +0.2 125,441 - +0.4 125,594 - GFF4827 0.19 +0.4 125,874 - -1.6 125,874 - -0.1 125,876 - +0.0 125,876 - -0.3 125,876 - +0.2 125,878 - -0.3 125,878 - +0.4 125,878 - -0.2 125,878 - +0.2 125,885 + -0.2 125,945 + GFF4829 0.32 -0.2 126,124 + +0.1 126,240 - +0.1 126,469 + GFF4830 0.84 +0.0 126,516 + -0.0 126,591 + GFF4831 0.10 -0.0 126,610 + GFF4831 0.13 -0.5 126,621 + GFF4831 0.15 -0.9
Or see this region's nucleotide sequence