Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4507 and GFF4508 are separated by 126 nucleotides GFF4508 and GFF4509 are separated by 0 nucleotides GFF4509 and GFF4510 overlap by 28 nucleotides
GFF4507 - Glycerol dehydrogenase (EC 1.1.1.6), at 293,890 to 294,978
GFF4507
GFF4508 - Aspartate aminotransferase (EC 2.6.1.1), at 295,105 to 296,265
GFF4508
GFF4509 - Co-activator of prophage gene expression IbrB, at 296,266 to 296,883
GFF4509
GFF4510 - Co-activator of prophage gene expression IbrA, at 296,856 to 297,965
GFF4510
Position (kb) 295
296
297 Strain fitness (log2 ratio) -1
0
1 at 294.230 kb on + strand, within GFF4507 at 294.460 kb on - strand, within GFF4507 at 294.482 kb on + strand, within GFF4507 at 294.482 kb on + strand, within GFF4507 at 294.483 kb on - strand, within GFF4507 at 294.582 kb on - strand, within GFF4507 at 294.582 kb on - strand, within GFF4507 at 294.707 kb on - strand, within GFF4507 at 294.825 kb on - strand, within GFF4507 at 295.352 kb on + strand, within GFF4508 at 295.353 kb on - strand, within GFF4508 at 295.417 kb on + strand, within GFF4508 at 295.486 kb on + strand, within GFF4508 at 295.529 kb on + strand, within GFF4508 at 295.624 kb on - strand, within GFF4508 at 295.725 kb on + strand, within GFF4508 at 295.725 kb on + strand, within GFF4508 at 295.725 kb on + strand, within GFF4508 at 295.726 kb on - strand, within GFF4508 at 295.821 kb on + strand, within GFF4508 at 295.894 kb on + strand, within GFF4508 at 295.895 kb on - strand, within GFF4508 at 296.131 kb on + strand, within GFF4508 at 296.197 kb on - strand at 296.199 kb on - strand at 296.199 kb on - strand at 296.199 kb on - strand at 296.263 kb on + strand at 296.271 kb on + strand at 296.361 kb on + strand, within GFF4509 at 296.398 kb on + strand, within GFF4509 at 296.665 kb on - strand, within GFF4509 at 296.715 kb on + strand, within GFF4509 at 296.724 kb on + strand, within GFF4509 at 296.788 kb on + strand, within GFF4509 at 296.788 kb on + strand, within GFF4509 at 296.789 kb on - strand, within GFF4509 at 296.883 kb on + strand at 296.916 kb on - strand at 296.916 kb on - strand at 297.116 kb on + strand, within GFF4510 at 297.146 kb on + strand, within GFF4510 at 297.147 kb on - strand, within GFF4510 at 297.153 kb on + strand, within GFF4510 at 297.242 kb on - strand, within GFF4510 at 297.242 kb on - strand, within GFF4510
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 294,230 + GFF4507 0.31 +0.1 294,460 - GFF4507 0.52 -0.1 294,482 + GFF4507 0.54 -0.1 294,482 + GFF4507 0.54 -0.5 294,483 - GFF4507 0.54 -0.1 294,582 - GFF4507 0.64 -0.1 294,582 - GFF4507 0.64 +0.4 294,707 - GFF4507 0.75 +0.0 294,825 - GFF4507 0.86 +0.7 295,352 + GFF4508 0.21 -0.1 295,353 - GFF4508 0.21 +0.1 295,417 + GFF4508 0.27 +0.4 295,486 + GFF4508 0.33 -0.3 295,529 + GFF4508 0.37 -0.4 295,624 - GFF4508 0.45 +0.6 295,725 + GFF4508 0.53 -0.6 295,725 + GFF4508 0.53 -1.3 295,725 + GFF4508 0.53 -0.1 295,726 - GFF4508 0.53 -0.2 295,821 + GFF4508 0.62 -0.2 295,894 + GFF4508 0.68 -1.0 295,895 - GFF4508 0.68 -0.2 296,131 + GFF4508 0.88 +0.2 296,197 - -0.3 296,199 - +0.0 296,199 - +0.7 296,199 - -0.1 296,263 + -0.1 296,271 + -0.5 296,361 + GFF4509 0.15 +0.1 296,398 + GFF4509 0.21 +0.5 296,665 - GFF4509 0.65 -0.8 296,715 + GFF4509 0.73 -0.3 296,724 + GFF4509 0.74 +0.1 296,788 + GFF4509 0.84 +0.3 296,788 + GFF4509 0.84 -0.1 296,789 - GFF4509 0.85 +0.1 296,883 + -0.4 296,916 - -0.4 296,916 - -0.3 297,116 + GFF4510 0.23 +1.1 297,146 + GFF4510 0.26 +0.6 297,147 - GFF4510 0.26 -0.4 297,153 + GFF4510 0.27 +0.4 297,242 - GFF4510 0.35 +0.3 297,242 - GFF4510 0.35 -0.1
Or see this region's nucleotide sequence