Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3990 and GFF3991 are separated by 246 nucleotides GFF3991 and GFF3992 are separated by 459 nucleotides GFF3992 and GFF3993 are separated by 442 nucleotides GFF3993 and GFF3994 are separated by 108 nucleotides
GFF3990 - hypothetical protein, at 265,050 to 265,169
GFF3990
GFF3991 - Cold shock protein CspG, at 265,416 to 265,628
GFF3991
GFF3992 - Outer membrane protein C precursor, at 266,088 to 267,284
GFF3992
GFF3993 - hypothetical protein, at 267,727 to 267,858
GFF3993
GFF3994 - FIG00638388: hypothetical protein, at 267,967 to 268,233
GFF3994
Position (kb) 266
267
268 Strain fitness (log2 ratio) -1
0
1 at 265.130 kb on - strand, within GFF3990 at 265.240 kb on + strand at 265.786 kb on + strand at 265.786 kb on + strand at 265.903 kb on + strand at 266.040 kb on - strand at 266.150 kb on - strand at 266.218 kb on + strand, within GFF3992 at 266.219 kb on - strand, within GFF3992 at 266.222 kb on - strand, within GFF3992 at 266.266 kb on + strand, within GFF3992 at 266.272 kb on + strand, within GFF3992 at 266.318 kb on + strand, within GFF3992 at 266.434 kb on + strand, within GFF3992 at 266.437 kb on + strand, within GFF3992 at 266.461 kb on + strand, within GFF3992 at 266.476 kb on + strand, within GFF3992 at 266.483 kb on + strand, within GFF3992 at 266.486 kb on - strand, within GFF3992 at 266.494 kb on + strand, within GFF3992 at 266.505 kb on + strand, within GFF3992 at 266.505 kb on + strand, within GFF3992 at 266.505 kb on + strand, within GFF3992 at 266.506 kb on - strand, within GFF3992 at 266.539 kb on + strand, within GFF3992 at 266.543 kb on + strand, within GFF3992 at 266.543 kb on + strand, within GFF3992 at 266.554 kb on + strand, within GFF3992 at 266.554 kb on + strand, within GFF3992 at 266.555 kb on - strand, within GFF3992 at 266.555 kb on - strand, within GFF3992 at 266.658 kb on - strand, within GFF3992 at 266.664 kb on - strand, within GFF3992 at 266.702 kb on + strand, within GFF3992 at 266.708 kb on + strand, within GFF3992 at 266.762 kb on + strand, within GFF3992 at 266.762 kb on + strand, within GFF3992 at 266.771 kb on - strand, within GFF3992 at 266.771 kb on - strand, within GFF3992 at 266.825 kb on - strand, within GFF3992 at 266.825 kb on - strand, within GFF3992 at 266.829 kb on - strand, within GFF3992 at 266.829 kb on - strand, within GFF3992 at 266.855 kb on + strand, within GFF3992 at 266.858 kb on - strand, within GFF3992 at 266.875 kb on + strand, within GFF3992 at 266.876 kb on - strand, within GFF3992 at 266.930 kb on + strand, within GFF3992 at 266.931 kb on - strand, within GFF3992 at 266.931 kb on - strand, within GFF3992 at 266.939 kb on - strand, within GFF3992 at 266.939 kb on - strand, within GFF3992 at 267.078 kb on - strand, within GFF3992 at 267.101 kb on + strand, within GFF3992 at 267.668 kb on - strand at 268.231 kb on + strand at 268.231 kb on + strand at 268.243 kb on + strand at 268.243 kb on + strand at 268.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 265,130 - GFF3990 0.67 +0.8 265,240 + -0.1 265,786 + -0.2 265,786 + +0.3 265,903 + +0.3 266,040 - -0.2 266,150 - +0.4 266,218 + GFF3992 0.11 +0.5 266,219 - GFF3992 0.11 +0.3 266,222 - GFF3992 0.11 +0.8 266,266 + GFF3992 0.15 -0.4 266,272 + GFF3992 0.15 +0.1 266,318 + GFF3992 0.19 +0.9 266,434 + GFF3992 0.29 -0.4 266,437 + GFF3992 0.29 -0.0 266,461 + GFF3992 0.31 +0.2 266,476 + GFF3992 0.32 +0.1 266,483 + GFF3992 0.33 +0.2 266,486 - GFF3992 0.33 +0.3 266,494 + GFF3992 0.34 +0.4 266,505 + GFF3992 0.35 -0.0 266,505 + GFF3992 0.35 +0.2 266,505 + GFF3992 0.35 +0.5 266,506 - GFF3992 0.35 -0.2 266,539 + GFF3992 0.38 +0.1 266,543 + GFF3992 0.38 -0.3 266,543 + GFF3992 0.38 +0.7 266,554 + GFF3992 0.39 -0.7 266,554 + GFF3992 0.39 -1.2 266,555 - GFF3992 0.39 +0.6 266,555 - GFF3992 0.39 +0.2 266,658 - GFF3992 0.48 -0.0 266,664 - GFF3992 0.48 +1.5 266,702 + GFF3992 0.51 +0.6 266,708 + GFF3992 0.52 +0.1 266,762 + GFF3992 0.56 -0.5 266,762 + GFF3992 0.56 +1.3 266,771 - GFF3992 0.57 +0.2 266,771 - GFF3992 0.57 +0.4 266,825 - GFF3992 0.62 +0.6 266,825 - GFF3992 0.62 -0.1 266,829 - GFF3992 0.62 -0.0 266,829 - GFF3992 0.62 +0.6 266,855 + GFF3992 0.64 +0.4 266,858 - GFF3992 0.64 +1.0 266,875 + GFF3992 0.66 -0.0 266,876 - GFF3992 0.66 +0.1 266,930 + GFF3992 0.70 -0.7 266,931 - GFF3992 0.70 +0.2 266,931 - GFF3992 0.70 +0.4 266,939 - GFF3992 0.71 +0.2 266,939 - GFF3992 0.71 -0.3 267,078 - GFF3992 0.83 +0.3 267,101 + GFF3992 0.85 -0.0 267,668 - -0.2 268,231 + +0.5 268,231 + +0.7 268,243 + +0.2 268,243 + +1.3 268,244 - -0.3
Or see this region's nucleotide sequence