Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3616 and GFF3617 are separated by 112 nucleotides GFF3617 and GFF3618 overlap by 4 nucleotides
GFF3616 - Putative transport protein, at 367,726 to 368,931
GFF3616
GFF3617 - LysR-family transcriptional regulator YdhB, at 369,044 to 369,976
GFF3617
GFF3618 - Purine nucleotide synthesis repressor, at 369,973 to 370,998
GFF3618
Position (kb) 369
370 Strain fitness (log2 ratio) -1
0
1 at 368.099 kb on + strand, within GFF3616 at 368.100 kb on - strand, within GFF3616 at 368.107 kb on + strand, within GFF3616 at 368.107 kb on + strand, within GFF3616 at 368.108 kb on - strand, within GFF3616 at 368.201 kb on + strand, within GFF3616 at 368.201 kb on + strand, within GFF3616 at 368.332 kb on + strand, within GFF3616 at 368.356 kb on - strand, within GFF3616 at 368.356 kb on - strand, within GFF3616 at 368.424 kb on + strand, within GFF3616 at 368.695 kb on - strand, within GFF3616 at 368.755 kb on + strand, within GFF3616 at 368.755 kb on + strand, within GFF3616 at 368.858 kb on + strand at 368.858 kb on + strand at 368.859 kb on - strand at 368.859 kb on - strand at 368.909 kb on - strand at 368.932 kb on - strand at 369.193 kb on - strand, within GFF3617 at 369.324 kb on + strand, within GFF3617 at 369.328 kb on + strand, within GFF3617 at 369.329 kb on - strand, within GFF3617 at 369.903 kb on - strand at 370.084 kb on + strand, within GFF3618 at 370.090 kb on - strand, within GFF3618 at 370.090 kb on - strand, within GFF3618 at 370.093 kb on - strand, within GFF3618 at 370.320 kb on - strand, within GFF3618 at 370.500 kb on - strand, within GFF3618 at 370.581 kb on + strand, within GFF3618 at 370.679 kb on + strand, within GFF3618 at 370.680 kb on - strand, within GFF3618 at 370.808 kb on - strand, within GFF3618 at 370.879 kb on - strand, within GFF3618 at 370.937 kb on - strand at 370.945 kb on + strand at 370.953 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 368,099 + GFF3616 0.31 -0.1 368,100 - GFF3616 0.31 +0.1 368,107 + GFF3616 0.32 +1.1 368,107 + GFF3616 0.32 -0.7 368,108 - GFF3616 0.32 -0.2 368,201 + GFF3616 0.39 -0.7 368,201 + GFF3616 0.39 +0.7 368,332 + GFF3616 0.50 -0.6 368,356 - GFF3616 0.52 +0.6 368,356 - GFF3616 0.52 +0.1 368,424 + GFF3616 0.58 +0.2 368,695 - GFF3616 0.80 +0.1 368,755 + GFF3616 0.85 -0.0 368,755 + GFF3616 0.85 +0.6 368,858 + +0.2 368,858 + -1.6 368,859 - +0.3 368,859 - -0.2 368,909 - +0.6 368,932 - +0.1 369,193 - GFF3617 0.16 -0.1 369,324 + GFF3617 0.30 +0.1 369,328 + GFF3617 0.30 +0.4 369,329 - GFF3617 0.31 +0.1 369,903 - -0.2 370,084 + GFF3618 0.11 +0.3 370,090 - GFF3618 0.11 -1.2 370,090 - GFF3618 0.11 -1.2 370,093 - GFF3618 0.12 +0.8 370,320 - GFF3618 0.34 +0.1 370,500 - GFF3618 0.51 -0.2 370,581 + GFF3618 0.59 -0.4 370,679 + GFF3618 0.69 +0.8 370,680 - GFF3618 0.69 +0.4 370,808 - GFF3618 0.81 -0.6 370,879 - GFF3618 0.88 -1.2 370,937 - -0.1 370,945 + -0.7 370,953 + +0.2
Or see this region's nucleotide sequence