Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3612 and GFF3649 are separated by 157 nucleotides GFF3649 and GFF3650 are separated by 10 nucleotides GFF3650 and GFF3613 are separated by 151 nucleotides GFF3613 and GFF3614 are separated by 216 nucleotides GFF3614 and GFF3615 are separated by 41 nucleotides
GFF3612 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 362,484 to 363,542
GFF3612
GFF3649 - tRNA-Val-GAC, at 363,700 to 363,776
GFF3649
GFF3650 - tRNA-Val-GAC, at 363,787 to 363,863
GFF3650
GFF3613 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 364,015 to 365,388
GFF3613
GFF3614 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 365,605 to 366,246
GFF3614
GFF3615 - Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79), at 366,288 to 367,436
GFF3615
Position (kb) 364
365
366 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 363.259 kb on + strand, within GFF3612 at 363.362 kb on + strand, within GFF3612 at 363.597 kb on - strand at 363.647 kb on - strand at 363.856 kb on - strand at 364.014 kb on - strand at 364.061 kb on - strand at 364.061 kb on - strand at 364.179 kb on - strand, within GFF3613 at 364.245 kb on + strand, within GFF3613 at 364.279 kb on + strand, within GFF3613 at 364.319 kb on - strand, within GFF3613 at 364.319 kb on - strand, within GFF3613 at 364.359 kb on - strand, within GFF3613 at 364.371 kb on + strand, within GFF3613 at 364.416 kb on + strand, within GFF3613 at 364.752 kb on + strand, within GFF3613 at 365.038 kb on - strand, within GFF3613 at 365.043 kb on + strand, within GFF3613 at 365.198 kb on - strand, within GFF3613 at 365.354 kb on - strand at 365.356 kb on + strand at 365.379 kb on - strand at 365.413 kb on - strand at 365.559 kb on - strand at 366.254 kb on + strand at 366.330 kb on + strand at 366.388 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 363,259 + GFF3612 0.73 -0.4 363,362 + GFF3612 0.83 -0.2 363,597 - +0.2 363,647 - +3.2 363,856 - -0.8 364,014 - +0.7 364,061 - +0.1 364,061 - -0.1 364,179 - GFF3613 0.12 -0.7 364,245 + GFF3613 0.17 -1.2 364,279 + GFF3613 0.19 -0.1 364,319 - GFF3613 0.22 -0.2 364,319 - GFF3613 0.22 -2.8 364,359 - GFF3613 0.25 +0.4 364,371 + GFF3613 0.26 -0.1 364,416 + GFF3613 0.29 +0.3 364,752 + GFF3613 0.54 -0.2 365,038 - GFF3613 0.74 -0.6 365,043 + GFF3613 0.75 +0.3 365,198 - GFF3613 0.86 +1.2 365,354 - +0.1 365,356 + -0.4 365,379 - -0.1 365,413 - -0.4 365,559 - -0.5 366,254 + +0.2 366,330 + -0.2 366,388 + +0.0
Or see this region's nucleotide sequence