Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3533 and GFF3534 are separated by 61 nucleotides GFF3534 and GFF3535 are separated by 34 nucleotides GFF3535 and GFF3536 are separated by 156 nucleotides
GFF3533 - FIG00638687: hypothetical protein, at 284,851 to 285,564
GFF3533
GFF3534 - Selenoprotein O and cysteine-containing homologs, at 285,626 to 287,068
GFF3534
GFF3535 - Hemin uptake protein HemP, at 287,103 to 287,294
GFF3535
GFF3536 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 287,451 to 288,497
GFF3536
Position (kb) 285
286
287
288 Strain fitness (log2 ratio) -2
-1
0
1 at 285.084 kb on + strand, within GFF3533 at 285.137 kb on + strand, within GFF3533 at 285.457 kb on - strand, within GFF3533 at 285.495 kb on + strand at 285.604 kb on + strand at 285.604 kb on + strand at 285.605 kb on - strand at 285.675 kb on - strand at 285.687 kb on + strand at 285.810 kb on + strand, within GFF3534 at 285.810 kb on + strand, within GFF3534 at 285.811 kb on - strand, within GFF3534 at 285.811 kb on - strand, within GFF3534 at 285.840 kb on + strand, within GFF3534 at 285.840 kb on + strand, within GFF3534 at 285.842 kb on + strand, within GFF3534 at 285.842 kb on + strand, within GFF3534 at 285.843 kb on - strand, within GFF3534 at 285.963 kb on + strand, within GFF3534 at 285.971 kb on + strand, within GFF3534 at 286.046 kb on + strand, within GFF3534 at 286.063 kb on + strand, within GFF3534 at 286.079 kb on - strand, within GFF3534 at 286.188 kb on - strand, within GFF3534 at 286.400 kb on + strand, within GFF3534 at 286.400 kb on + strand, within GFF3534 at 286.428 kb on - strand, within GFF3534 at 286.596 kb on + strand, within GFF3534 at 286.639 kb on + strand, within GFF3534 at 286.709 kb on + strand, within GFF3534 at 286.927 kb on - strand at 287.013 kb on - strand at 287.119 kb on - strand at 287.380 kb on - strand at 287.501 kb on + strand at 287.542 kb on + strand at 287.649 kb on - strand, within GFF3536 at 287.658 kb on + strand, within GFF3536 at 287.659 kb on - strand, within GFF3536 at 287.664 kb on + strand, within GFF3536 at 287.840 kb on - strand, within GFF3536 at 287.844 kb on - strand, within GFF3536 at 287.879 kb on + strand, within GFF3536 at 287.881 kb on - strand, within GFF3536 at 288.063 kb on - strand, within GFF3536
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 285,084 + GFF3533 0.33 -1.8 285,137 + GFF3533 0.40 -0.9 285,457 - GFF3533 0.85 -0.1 285,495 + -0.4 285,604 + -0.5 285,604 + -0.1 285,605 - +0.4 285,675 - -0.1 285,687 + +0.5 285,810 + GFF3534 0.13 -0.1 285,810 + GFF3534 0.13 +0.3 285,811 - GFF3534 0.13 +0.5 285,811 - GFF3534 0.13 +0.3 285,840 + GFF3534 0.15 +0.0 285,840 + GFF3534 0.15 -0.3 285,842 + GFF3534 0.15 -0.2 285,842 + GFF3534 0.15 +0.4 285,843 - GFF3534 0.15 +0.3 285,963 + GFF3534 0.23 -0.4 285,971 + GFF3534 0.24 +0.1 286,046 + GFF3534 0.29 +0.3 286,063 + GFF3534 0.30 -0.6 286,079 - GFF3534 0.31 -0.3 286,188 - GFF3534 0.39 +0.4 286,400 + GFF3534 0.54 +0.0 286,400 + GFF3534 0.54 +0.0 286,428 - GFF3534 0.56 -0.1 286,596 + GFF3534 0.67 -0.4 286,639 + GFF3534 0.70 -0.1 286,709 + GFF3534 0.75 +0.2 286,927 - -0.3 287,013 - -0.4 287,119 - +0.4 287,380 - -0.7 287,501 + +1.2 287,542 + -0.5 287,649 - GFF3536 0.19 +0.1 287,658 + GFF3536 0.20 -0.7 287,659 - GFF3536 0.20 -0.4 287,664 + GFF3536 0.20 +0.3 287,840 - GFF3536 0.37 +0.6 287,844 - GFF3536 0.38 +0.3 287,879 + GFF3536 0.41 -0.1 287,881 - GFF3536 0.41 -0.3 288,063 - GFF3536 0.58 -0.1
Or see this region's nucleotide sequence