Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF28 and GFF29 are separated by 31 nucleotides GFF29 and GFF30 overlap by 4 nucleotides GFF30 and GFF31 are separated by 197 nucleotides GFF31 and GFF32 are separated by 52 nucleotides GFF32 and GFF33 are separated by 83 nucleotides
GFF28 - Copper homeostasis protein CutF precursor / Lipoprotein NlpE involeved in surface adhesion, at 6,871 to 7,401
GFF28
GFF29 - Hypothetical protein YaeJ with similarity to translation release factor, at 7,433 to 7,855
GFF29
GFF30 - YaeQ protein, at 7,852 to 8,397
GFF30
GFF31 - FIG00639395: hypothetical protein, at 8,595 to 8,795
GFF31
GFF32 - Rho-specific inhibitor of transcription termination (YaeO), at 8,848 to 9,042
GFF32
GFF33 - tRNA(Ile)-lysidine synthetase (EC 6.3.4.19), at 9,126 to 10,418
GFF33
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-1
0
1
2 at 6.937 kb on - strand, within GFF28 at 6.937 kb on - strand, within GFF28 at 6.937 kb on - strand, within GFF28 at 6.937 kb on - strand, within GFF28 at 6.937 kb on - strand, within GFF28 at 6.940 kb on + strand, within GFF28 at 6.940 kb on + strand, within GFF28 at 6.941 kb on - strand, within GFF28 at 6.941 kb on - strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.944 kb on + strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.945 kb on - strand, within GFF28 at 6.948 kb on + strand, within GFF28 at 6.948 kb on + strand, within GFF28 at 6.948 kb on + strand, within GFF28 at 6.949 kb on - strand, within GFF28 at 7.005 kb on - strand, within GFF28 at 7.008 kb on + strand, within GFF28 at 7.074 kb on - strand, within GFF28 at 7.140 kb on - strand, within GFF28 at 7.219 kb on + strand, within GFF28 at 7.339 kb on - strand, within GFF28 at 7.363 kb on - strand at 7.384 kb on + strand at 7.385 kb on - strand at 7.473 kb on - strand at 7.702 kb on - strand, within GFF29 at 7.762 kb on + strand, within GFF29 at 7.763 kb on - strand, within GFF29 at 8.078 kb on + strand, within GFF30 at 8.250 kb on - strand, within GFF30 at 8.373 kb on + strand at 8.445 kb on + strand at 8.511 kb on + strand at 8.512 kb on - strand at 8.512 kb on - strand at 8.513 kb on + strand at 8.513 kb on + strand at 8.513 kb on + strand at 8.513 kb on + strand at 8.513 kb on + strand at 8.514 kb on - strand at 8.514 kb on - strand at 8.526 kb on - strand at 8.551 kb on - strand at 8.604 kb on + strand at 8.617 kb on + strand, within GFF31 at 8.653 kb on + strand, within GFF31 at 8.658 kb on + strand, within GFF31 at 8.696 kb on + strand, within GFF31 at 8.781 kb on + strand at 8.800 kb on + strand at 8.825 kb on - strand at 8.829 kb on + strand at 8.830 kb on - strand at 8.859 kb on - strand at 8.938 kb on + strand, within GFF32
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 6,937 - GFF28 0.12 -0.8 6,937 - GFF28 0.12 -0.1 6,937 - GFF28 0.12 +0.8 6,937 - GFF28 0.12 -1.1 6,937 - GFF28 0.12 -1.2 6,940 + GFF28 0.13 +1.8 6,940 + GFF28 0.13 -1.1 6,941 - GFF28 0.13 +1.5 6,941 - GFF28 0.13 +0.4 6,944 + GFF28 0.14 -1.7 6,944 + GFF28 0.14 +0.2 6,944 + GFF28 0.14 +0.3 6,944 + GFF28 0.14 -0.1 6,944 + GFF28 0.14 -0.2 6,945 - GFF28 0.14 +0.5 6,945 - GFF28 0.14 +1.0 6,945 - GFF28 0.14 +0.2 6,945 - GFF28 0.14 +0.0 6,945 - GFF28 0.14 +0.2 6,945 - GFF28 0.14 +0.1 6,948 + GFF28 0.15 +0.4 6,948 + GFF28 0.15 +0.4 6,948 + GFF28 0.15 -0.9 6,949 - GFF28 0.15 +0.4 7,005 - GFF28 0.25 +0.5 7,008 + GFF28 0.26 -0.9 7,074 - GFF28 0.38 +0.4 7,140 - GFF28 0.51 +0.0 7,219 + GFF28 0.66 +0.1 7,339 - GFF28 0.88 -0.4 7,363 - +0.1 7,384 + -0.0 7,385 - +0.5 7,473 - -0.3 7,702 - GFF29 0.64 -0.2 7,762 + GFF29 0.78 -0.5 7,763 - GFF29 0.78 +0.4 8,078 + GFF30 0.41 +0.3 8,250 - GFF30 0.73 +0.7 8,373 + +0.0 8,445 + -0.7 8,511 + -0.4 8,512 - -1.1 8,512 - -0.9 8,513 + -0.1 8,513 + +0.3 8,513 + -0.5 8,513 + -0.4 8,513 + -0.2 8,514 - +0.1 8,514 - -0.2 8,526 - +1.4 8,551 - +0.0 8,604 + -1.5 8,617 + GFF31 0.11 -0.8 8,653 + GFF31 0.29 +0.2 8,658 + GFF31 0.31 -0.8 8,696 + GFF31 0.50 +0.1 8,781 + -0.5 8,800 + +0.2 8,825 - -0.6 8,829 + +0.0 8,830 - -0.1 8,859 - -0.4 8,938 + GFF32 0.46 -0.1
Or see this region's nucleotide sequence