Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2649 and GFF2650 are separated by 486 nucleotides GFF2650 and GFF2651 are separated by 145 nucleotides
GFF2649 - ATP-dependent 23S rRNA helicase DbpA, at 173,906 to 175,279
GFF2649
GFF2650 - Zinc transport protein ZntB, at 175,766 to 176,749
GFF2650
GFF2651 - Putative methyl-accepting chemotaxis protein, at 176,895 to 178,007
GFF2651
Position (kb) 175
176
177 Strain fitness (log2 ratio) -1
0
1
2 at 175.056 kb on - strand, within GFF2649 at 175.056 kb on - strand, within GFF2649 at 175.462 kb on - strand at 175.467 kb on + strand at 175.467 kb on + strand at 175.468 kb on - strand at 175.468 kb on - strand at 175.491 kb on - strand at 175.491 kb on - strand at 175.543 kb on - strand at 175.543 kb on - strand at 175.563 kb on + strand at 175.582 kb on - strand at 175.945 kb on + strand, within GFF2650 at 175.947 kb on + strand, within GFF2650 at 176.127 kb on + strand, within GFF2650 at 176.151 kb on + strand, within GFF2650 at 176.180 kb on + strand, within GFF2650 at 176.267 kb on - strand, within GFF2650 at 176.420 kb on - strand, within GFF2650 at 176.421 kb on + strand, within GFF2650 at 176.421 kb on + strand, within GFF2650 at 176.421 kb on + strand, within GFF2650 at 176.422 kb on - strand, within GFF2650 at 176.470 kb on + strand, within GFF2650 at 176.513 kb on + strand, within GFF2650 at 176.608 kb on + strand, within GFF2650 at 176.610 kb on + strand, within GFF2650 at 176.611 kb on - strand, within GFF2650 at 176.615 kb on - strand, within GFF2650 at 176.621 kb on + strand, within GFF2650 at 176.622 kb on - strand, within GFF2650 at 176.656 kb on + strand at 176.656 kb on + strand at 176.934 kb on + strand at 176.934 kb on + strand at 176.935 kb on - strand at 176.935 kb on - strand at 176.935 kb on - strand at 177.027 kb on + strand, within GFF2651 at 177.028 kb on - strand, within GFF2651 at 177.111 kb on + strand, within GFF2651 at 177.228 kb on + strand, within GFF2651 at 177.471 kb on - strand, within GFF2651 at 177.546 kb on + strand, within GFF2651 at 177.603 kb on + strand, within GFF2651 at 177.652 kb on - strand, within GFF2651
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 175,056 - GFF2649 0.84 +0.1 175,056 - GFF2649 0.84 +0.5 175,462 - +0.3 175,467 + -0.0 175,467 + -0.2 175,468 - +0.7 175,468 - +0.7 175,491 - +0.5 175,491 - -0.5 175,543 - -0.2 175,543 - -0.4 175,563 + -0.2 175,582 - -0.0 175,945 + GFF2650 0.18 -0.1 175,947 + GFF2650 0.18 +0.8 176,127 + GFF2650 0.37 +0.3 176,151 + GFF2650 0.39 +0.1 176,180 + GFF2650 0.42 +0.4 176,267 - GFF2650 0.51 -0.2 176,420 - GFF2650 0.66 +1.8 176,421 + GFF2650 0.67 +0.7 176,421 + GFF2650 0.67 +0.5 176,421 + GFF2650 0.67 -0.2 176,422 - GFF2650 0.67 -0.1 176,470 + GFF2650 0.72 +0.2 176,513 + GFF2650 0.76 +0.0 176,608 + GFF2650 0.86 +0.1 176,610 + GFF2650 0.86 +0.2 176,611 - GFF2650 0.86 -0.9 176,615 - GFF2650 0.86 +0.3 176,621 + GFF2650 0.87 +1.6 176,622 - GFF2650 0.87 -0.2 176,656 + +0.5 176,656 + -0.9 176,934 + +0.2 176,934 + -0.3 176,935 - -0.2 176,935 - -0.6 176,935 - +0.6 177,027 + GFF2651 0.12 +0.7 177,028 - GFF2651 0.12 -0.7 177,111 + GFF2651 0.19 +1.8 177,228 + GFF2651 0.30 +0.9 177,471 - GFF2651 0.52 +0.1 177,546 + GFF2651 0.58 +0.4 177,603 + GFF2651 0.64 +0.9 177,652 - GFF2651 0.68 +0.2
Or see this region's nucleotide sequence