Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2577 and GFF2578 are separated by 263 nucleotides GFF2578 and GFF2579 are separated by 66 nucleotides
GFF2577 - FIG00638561: hypothetical protein, at 98,203 to 99,264
GFF2577
GFF2578 - Probable tonB-dependent receptor yncD precursor, at 99,528 to 101,648
GFF2578
GFF2579 - Transcriptional regulator, GntR family, at 101,715 to 102,380
GFF2579
Position (kb) 99
100
101
102 Strain fitness (log2 ratio) -1
0
1 at 98.565 kb on + strand, within GFF2577 at 98.565 kb on + strand, within GFF2577 at 98.602 kb on - strand, within GFF2577 at 98.769 kb on + strand, within GFF2577 at 98.770 kb on - strand, within GFF2577 at 98.799 kb on - strand, within GFF2577 at 98.863 kb on - strand, within GFF2577 at 98.863 kb on - strand, within GFF2577 at 98.896 kb on + strand, within GFF2577 at 98.897 kb on - strand, within GFF2577 at 98.897 kb on - strand, within GFF2577 at 99.056 kb on - strand, within GFF2577 at 99.056 kb on - strand, within GFF2577 at 99.187 kb on - strand at 99.215 kb on - strand at 99.220 kb on + strand at 99.256 kb on + strand at 99.257 kb on - strand at 99.257 kb on - strand at 99.290 kb on + strand at 99.290 kb on + strand at 99.291 kb on - strand at 99.292 kb on + strand at 99.293 kb on - strand at 99.293 kb on - strand at 99.357 kb on + strand at 99.515 kb on - strand at 99.672 kb on - strand at 99.789 kb on + strand, within GFF2578 at 99.864 kb on + strand, within GFF2578 at 99.998 kb on - strand, within GFF2578 at 100.001 kb on - strand, within GFF2578 at 100.135 kb on - strand, within GFF2578 at 100.137 kb on + strand, within GFF2578 at 100.137 kb on + strand, within GFF2578 at 100.354 kb on - strand, within GFF2578 at 100.359 kb on - strand, within GFF2578 at 100.478 kb on + strand, within GFF2578 at 100.583 kb on - strand, within GFF2578 at 101.156 kb on + strand, within GFF2578 at 101.334 kb on - strand, within GFF2578 at 101.525 kb on + strand at 101.544 kb on + strand at 101.548 kb on + strand at 101.586 kb on + strand at 101.953 kb on + strand, within GFF2579 at 102.165 kb on - strand, within GFF2579 at 102.580 kb on - strand at 102.618 kb on - strand at 102.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 98,565 + GFF2577 0.34 -0.1 98,565 + GFF2577 0.34 +0.3 98,602 - GFF2577 0.38 +0.2 98,769 + GFF2577 0.53 -0.4 98,770 - GFF2577 0.53 +0.1 98,799 - GFF2577 0.56 +0.2 98,863 - GFF2577 0.62 -0.5 98,863 - GFF2577 0.62 -0.0 98,896 + GFF2577 0.65 +0.2 98,897 - GFF2577 0.65 -0.1 98,897 - GFF2577 0.65 +0.8 99,056 - GFF2577 0.80 -0.0 99,056 - GFF2577 0.80 -0.2 99,187 - -0.6 99,215 - +0.3 99,220 + +0.5 99,256 + -0.2 99,257 - +0.7 99,257 - -0.0 99,290 + +0.5 99,290 + -1.0 99,291 - -0.1 99,292 + +1.0 99,293 - -0.3 99,293 - +0.5 99,357 + -0.2 99,515 - -0.4 99,672 - -0.1 99,789 + GFF2578 0.12 +0.3 99,864 + GFF2578 0.16 -0.2 99,998 - GFF2578 0.22 -0.7 100,001 - GFF2578 0.22 -0.7 100,135 - GFF2578 0.29 -0.9 100,137 + GFF2578 0.29 -0.2 100,137 + GFF2578 0.29 -0.3 100,354 - GFF2578 0.39 -0.5 100,359 - GFF2578 0.39 +0.7 100,478 + GFF2578 0.45 -1.0 100,583 - GFF2578 0.50 +0.3 101,156 + GFF2578 0.77 -0.5 101,334 - GFF2578 0.85 -0.4 101,525 + -0.2 101,544 + +0.2 101,548 + -0.6 101,586 + -0.2 101,953 + GFF2579 0.36 -0.3 102,165 - GFF2579 0.68 +0.4 102,580 - -0.2 102,618 - -0.1 102,618 - -1.2
Or see this region's nucleotide sequence