Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2472 and GFF2473 are separated by 11 nucleotides GFF2473 and GFF2474 are separated by 11 nucleotides GFF2474 and GFF2475 overlap by 4 nucleotides GFF2475 and GFF2476 are separated by 216 nucleotides
GFF2472 - 'Pyridoxine 5'-phosphate synthase (EC 2.6.99.2)' transl_table=11, at 90,927 to 91,658
GFF2472
GFF2473 - DNA recombination and repair protein RecO, at 91,670 to 92,398
GFF2473
GFF2474 - GTP-binding protein Era, at 92,410 to 93,315
GFF2474
GFF2475 - Ribonuclease III (EC 3.1.26.3), at 93,312 to 94,049
GFF2475
GFF2476 - Signal peptidase I (EC 3.4.21.89), at 94,266 to 95,240
GFF2476
Position (kb) 92
93
94 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 91.761 kb on - strand, within GFF2473 at 91.802 kb on + strand, within GFF2473 at 91.802 kb on + strand, within GFF2473 at 91.802 kb on + strand, within GFF2473 at 91.802 kb on + strand, within GFF2473 at 92.137 kb on + strand, within GFF2473 at 92.221 kb on - strand, within GFF2473 at 92.423 kb on - strand at 92.423 kb on - strand at 93.473 kb on - strand, within GFF2475 at 93.759 kb on - strand, within GFF2475 at 93.824 kb on - strand, within GFF2475 at 94.046 kb on - strand at 94.046 kb on - strand at 94.158 kb on - strand at 94.163 kb on - strand at 94.213 kb on + strand at 94.274 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 91,761 - GFF2473 0.12 -0.4 91,802 + GFF2473 0.18 -0.8 91,802 + GFF2473 0.18 -0.5 91,802 + GFF2473 0.18 +0.2 91,802 + GFF2473 0.18 -0.8 92,137 + GFF2473 0.64 +0.2 92,221 - GFF2473 0.76 +0.7 92,423 - -0.1 92,423 - -0.5 93,473 - GFF2475 0.22 -2.7 93,759 - GFF2475 0.61 -3.1 93,824 - GFF2475 0.69 -3.3 94,046 - -0.4 94,046 - -1.1 94,158 - -0.4 94,163 - -0.3 94,213 + +0.0 94,274 + -0.8
Or see this region's nucleotide sequence