Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2055 and GFF2056 are separated by 67 nucleotides GFF2056 and GFF2057 overlap by 11 nucleotides GFF2057 and GFF2058 overlap by 1 nucleotides
GFF2055 - Cysteine synthase (EC 2.5.1.47), at 19,670 to 20,581
GFF2055
GFF2056 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 20,649 to 21,746
GFF2056
GFF2057 - Sulfate transport system permease protein CysW, at 21,736 to 22,611
GFF2057
GFF2058 - Sulfate transport system permease protein CysT, at 22,611 to 23,444
GFF2058
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-1
0
1
2 at 19.671 kb on + strand at 19.905 kb on - strand, within GFF2055 at 19.933 kb on + strand, within GFF2055 at 19.934 kb on - strand, within GFF2055 at 19.934 kb on - strand, within GFF2055 at 20.197 kb on + strand, within GFF2055 at 20.207 kb on + strand, within GFF2055 at 20.240 kb on - strand, within GFF2055 at 20.570 kb on + strand at 20.571 kb on - strand at 20.571 kb on - strand at 20.575 kb on + strand at 20.634 kb on - strand at 20.832 kb on - strand, within GFF2056 at 20.834 kb on - strand, within GFF2056 at 20.865 kb on + strand, within GFF2056 at 20.872 kb on - strand, within GFF2056 at 20.903 kb on + strand, within GFF2056 at 20.957 kb on + strand, within GFF2056 at 21.105 kb on - strand, within GFF2056 at 21.201 kb on + strand, within GFF2056 at 21.222 kb on + strand, within GFF2056 at 21.524 kb on + strand, within GFF2056 at 21.573 kb on - strand, within GFF2056 at 21.832 kb on + strand, within GFF2057 at 21.875 kb on + strand, within GFF2057 at 21.901 kb on + strand, within GFF2057 at 22.003 kb on + strand, within GFF2057 at 22.004 kb on - strand, within GFF2057 at 22.066 kb on + strand, within GFF2057 at 22.310 kb on - strand, within GFF2057 at 22.310 kb on - strand, within GFF2057 at 22.620 kb on + strand at 22.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 19,671 + +0.5 19,905 - GFF2055 0.26 +0.2 19,933 + GFF2055 0.29 -0.5 19,934 - GFF2055 0.29 +0.1 19,934 - GFF2055 0.29 -0.2 20,197 + GFF2055 0.58 +0.3 20,207 + GFF2055 0.59 +0.4 20,240 - GFF2055 0.62 +0.2 20,570 + -0.5 20,571 - -0.2 20,571 - +0.7 20,575 + -0.7 20,634 - +0.6 20,832 - GFF2056 0.17 +0.0 20,834 - GFF2056 0.17 -0.7 20,865 + GFF2056 0.20 -0.3 20,872 - GFF2056 0.20 -0.4 20,903 + GFF2056 0.23 +0.3 20,957 + GFF2056 0.28 -0.2 21,105 - GFF2056 0.42 -0.0 21,201 + GFF2056 0.50 -0.3 21,222 + GFF2056 0.52 -0.2 21,524 + GFF2056 0.80 -0.6 21,573 - GFF2056 0.84 -0.4 21,832 + GFF2057 0.11 +2.1 21,875 + GFF2057 0.16 -0.3 21,901 + GFF2057 0.19 -0.3 22,003 + GFF2057 0.30 -0.8 22,004 - GFF2057 0.31 -1.0 22,066 + GFF2057 0.38 +0.2 22,310 - GFF2057 0.66 +0.4 22,310 - GFF2057 0.66 -0.0 22,620 + -0.2 22,621 - -0.4
Or see this region's nucleotide sequence