Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1730 and GFF1731 are separated by 150 nucleotides GFF1731 and GFF1732 are separated by 61 nucleotides GFF1732 and GFF1733 are separated by 48 nucleotides
GFF1730 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 28,975 to 30,804
GFF1730
GFF1731 - SgrR, sugar-phosphate stress, transcriptional activator of SgrS small RNA, at 30,955 to 32,745
GFF1731
GFF1732 - Shikimate 5-dehydrogenase I gamma (EC 1.1.1.25), at 32,807 to 33,625
GFF1732
GFF1733 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 33,674 to 35,047
GFF1733
Position (kb) 30
31
32
33 Strain fitness (log2 ratio) -1
0
1 at 30.802 kb on + strand at 30.803 kb on - strand at 30.803 kb on - strand at 30.806 kb on + strand at 30.806 kb on + strand at 30.806 kb on + strand at 30.807 kb on - strand at 30.814 kb on + strand at 30.815 kb on - strand at 31.005 kb on + strand at 31.006 kb on - strand at 31.324 kb on + strand, within GFF1731 at 31.325 kb on - strand, within GFF1731 at 31.467 kb on + strand, within GFF1731 at 31.467 kb on + strand, within GFF1731 at 31.622 kb on - strand, within GFF1731 at 31.682 kb on - strand, within GFF1731 at 31.690 kb on - strand, within GFF1731 at 31.975 kb on - strand, within GFF1731 at 32.239 kb on + strand, within GFF1731 at 32.239 kb on + strand, within GFF1731 at 32.300 kb on - strand, within GFF1731 at 32.372 kb on + strand, within GFF1731 at 32.372 kb on + strand, within GFF1731 at 32.413 kb on - strand, within GFF1731 at 32.413 kb on - strand, within GFF1731 at 32.482 kb on + strand, within GFF1731 at 32.483 kb on - strand, within GFF1731 at 32.499 kb on + strand, within GFF1731 at 32.595 kb on + strand at 32.635 kb on + strand at 32.635 kb on + strand at 32.635 kb on + strand at 32.635 kb on + strand at 32.674 kb on + strand at 32.680 kb on + strand at 32.680 kb on + strand at 32.681 kb on - strand at 32.891 kb on + strand, within GFF1732 at 32.891 kb on + strand, within GFF1732 at 32.919 kb on - strand, within GFF1732 at 32.919 kb on - strand, within GFF1732 at 32.925 kb on - strand, within GFF1732 at 33.077 kb on - strand, within GFF1732 at 33.077 kb on - strand, within GFF1732 at 33.077 kb on - strand, within GFF1732 at 33.091 kb on + strand, within GFF1732 at 33.125 kb on + strand, within GFF1732 at 33.156 kb on - strand, within GFF1732 at 33.203 kb on - strand, within GFF1732 at 33.257 kb on - strand, within GFF1732 at 33.408 kb on - strand, within GFF1732 at 33.439 kb on + strand, within GFF1732 at 33.536 kb on - strand, within GFF1732 at 33.536 kb on - strand, within GFF1732 at 33.563 kb on + strand at 33.673 kb on + strand at 33.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 30,802 + -0.1 30,803 - -0.2 30,803 - -0.2 30,806 + +0.2 30,806 + +0.9 30,806 + +0.4 30,807 - -0.8 30,814 + +0.8 30,815 - +0.2 31,005 + -0.1 31,006 - +0.6 31,324 + GFF1731 0.21 +0.7 31,325 - GFF1731 0.21 -0.6 31,467 + GFF1731 0.29 +0.5 31,467 + GFF1731 0.29 +0.4 31,622 - GFF1731 0.37 +0.5 31,682 - GFF1731 0.41 -0.0 31,690 - GFF1731 0.41 +0.5 31,975 - GFF1731 0.57 -0.2 32,239 + GFF1731 0.72 +0.5 32,239 + GFF1731 0.72 -0.0 32,300 - GFF1731 0.75 +0.8 32,372 + GFF1731 0.79 +0.4 32,372 + GFF1731 0.79 +0.3 32,413 - GFF1731 0.81 +0.8 32,413 - GFF1731 0.81 -0.7 32,482 + GFF1731 0.85 +0.4 32,483 - GFF1731 0.85 +0.6 32,499 + GFF1731 0.86 -0.6 32,595 + +1.0 32,635 + +0.7 32,635 + -0.4 32,635 + -0.0 32,635 + -0.2 32,674 + -0.3 32,680 + -0.1 32,680 + -0.0 32,681 - +0.3 32,891 + GFF1732 0.10 +0.3 32,891 + GFF1732 0.10 -0.4 32,919 - GFF1732 0.14 -0.6 32,919 - GFF1732 0.14 -0.0 32,925 - GFF1732 0.14 -0.4 33,077 - GFF1732 0.33 -0.7 33,077 - GFF1732 0.33 -0.5 33,077 - GFF1732 0.33 +0.5 33,091 + GFF1732 0.35 +0.3 33,125 + GFF1732 0.39 +0.2 33,156 - GFF1732 0.43 -0.0 33,203 - GFF1732 0.48 -1.5 33,257 - GFF1732 0.55 -0.3 33,408 - GFF1732 0.73 +0.1 33,439 + GFF1732 0.77 +0.6 33,536 - GFF1732 0.89 +1.0 33,536 - GFF1732 0.89 -0.3 33,563 + -0.3 33,673 + -0.1 33,731 - +0.1
Or see this region's nucleotide sequence