Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1652 and GFF1653 are separated by 235 nucleotides GFF1653 and GFF1654 are separated by 19 nucleotides GFF1654 and GFF1655 are separated by 104 nucleotides GFF1655 and GFF1656 are separated by 15 nucleotides
GFF1652 - tRNA (5-methoxyuridine) 34 synthase, at 36,239 to 37,210
GFF1652
GFF1653 - Cytoplasmic copper homeostasis protein CutC, at 37,446 to 38,192
GFF1653
GFF1654 - Protein yecM, at 38,212 to 38,781
GFF1654
GFF1655 - hypothetical protein, at 38,886 to 39,002
GFF1655
GFF1656 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 39,018 to 40,751
GFF1656
Position (kb) 37
38
39 Strain fitness (log2 ratio) -1
0
1 at 36.479 kb on - strand, within GFF1652 at 36.539 kb on - strand, within GFF1652 at 36.641 kb on + strand, within GFF1652 at 36.946 kb on - strand, within GFF1652 at 36.992 kb on - strand, within GFF1652 at 37.051 kb on + strand, within GFF1652 at 37.231 kb on - strand at 37.234 kb on + strand at 37.234 kb on + strand at 37.234 kb on + strand at 37.234 kb on + strand at 37.255 kb on + strand at 37.302 kb on + strand at 37.302 kb on + strand at 37.310 kb on + strand at 37.418 kb on - strand at 37.503 kb on + strand at 37.509 kb on - strand at 37.509 kb on - strand at 37.519 kb on + strand at 37.710 kb on - strand, within GFF1653 at 37.886 kb on + strand, within GFF1653 at 38.031 kb on + strand, within GFF1653 at 38.042 kb on + strand, within GFF1653 at 38.046 kb on - strand, within GFF1653 at 38.065 kb on - strand, within GFF1653 at 38.185 kb on - strand at 38.301 kb on + strand, within GFF1654 at 38.347 kb on - strand, within GFF1654 at 38.367 kb on + strand, within GFF1654 at 38.571 kb on + strand, within GFF1654 at 38.584 kb on + strand, within GFF1654 at 38.585 kb on - strand, within GFF1654 at 38.865 kb on - strand at 38.876 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 36,479 - GFF1652 0.25 +0.2 36,539 - GFF1652 0.31 -0.3 36,641 + GFF1652 0.41 -0.1 36,946 - GFF1652 0.73 -0.6 36,992 - GFF1652 0.77 -0.5 37,051 + GFF1652 0.84 -0.0 37,231 - -0.0 37,234 + -0.2 37,234 + -1.0 37,234 + +0.0 37,234 + -0.3 37,255 + -0.1 37,302 + +0.0 37,302 + -0.2 37,310 + +1.2 37,418 - +0.5 37,503 + -0.5 37,509 - -0.4 37,509 - -0.1 37,519 + +0.2 37,710 - GFF1653 0.35 -1.2 37,886 + GFF1653 0.59 -0.3 38,031 + GFF1653 0.78 +0.3 38,042 + GFF1653 0.80 -0.1 38,046 - GFF1653 0.80 +0.2 38,065 - GFF1653 0.83 -0.4 38,185 - +0.7 38,301 + GFF1654 0.16 +0.1 38,347 - GFF1654 0.24 +0.3 38,367 + GFF1654 0.27 +1.0 38,571 + GFF1654 0.63 +0.2 38,584 + GFF1654 0.65 +0.3 38,585 - GFF1654 0.65 +0.3 38,865 - -0.1 38,876 - -1.0
Or see this region's nucleotide sequence