Experiment: LB_plus_SM_buffer with 6F2_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1478 and GFF1479 are separated by 146 nucleotides GFF1479 and GFF1480 are separated by 409 nucleotides GFF1480 and GFF1481 overlap by 4 nucleotides
GFF1478 - C4-dicarboxylate transporter DcuB, at 147,238 to 148,578
GFF1478
GFF1479 - hypothetical protein, at 148,725 to 148,898
GFF1479
GFF1480 - Fumarate respiration transcriptional regulator DcuR, at 149,308 to 150,027
GFF1480
GFF1481 - Fumarate respiration sensor kinase protein DcuS, at 150,024 to 151,655
GFF1481
Position (kb) 149
150
151 Strain fitness (log2 ratio) -1
0
1 at 148.309 kb on - strand, within GFF1478 at 148.309 kb on - strand, within GFF1478 at 148.309 kb on - strand, within GFF1478 at 148.316 kb on + strand, within GFF1478 at 148.317 kb on - strand, within GFF1478 at 148.427 kb on - strand, within GFF1478 at 148.544 kb on - strand at 148.937 kb on + strand at 149.318 kb on + strand at 149.527 kb on + strand, within GFF1480 at 149.527 kb on + strand, within GFF1480 at 149.665 kb on + strand, within GFF1480 at 149.666 kb on - strand, within GFF1480 at 149.735 kb on + strand, within GFF1480 at 149.751 kb on + strand, within GFF1480 at 149.853 kb on + strand, within GFF1480 at 149.855 kb on + strand, within GFF1480 at 149.856 kb on - strand, within GFF1480 at 149.856 kb on - strand, within GFF1480 at 149.970 kb on - strand at 150.013 kb on + strand at 150.060 kb on + strand at 150.265 kb on + strand, within GFF1481 at 150.271 kb on - strand, within GFF1481 at 150.360 kb on - strand, within GFF1481 at 150.381 kb on - strand, within GFF1481 at 150.381 kb on - strand, within GFF1481 at 150.406 kb on - strand, within GFF1481 at 150.515 kb on - strand, within GFF1481 at 150.518 kb on + strand, within GFF1481 at 150.519 kb on - strand, within GFF1481 at 150.536 kb on + strand, within GFF1481 at 150.538 kb on + strand, within GFF1481 at 150.540 kb on - strand, within GFF1481 at 150.555 kb on - strand, within GFF1481 at 150.556 kb on + strand, within GFF1481 at 150.557 kb on - strand, within GFF1481 at 150.557 kb on - strand, within GFF1481 at 150.592 kb on - strand, within GFF1481 at 150.637 kb on + strand, within GFF1481 at 150.687 kb on - strand, within GFF1481 at 150.743 kb on + strand, within GFF1481 at 150.818 kb on + strand, within GFF1481 at 150.915 kb on - strand, within GFF1481
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.01875 MOI remove 148,309 - GFF1478 0.80 +0.6 148,309 - GFF1478 0.80 +0.6 148,309 - GFF1478 0.80 -0.3 148,316 + GFF1478 0.80 -0.6 148,317 - GFF1478 0.80 +0.0 148,427 - GFF1478 0.89 +1.2 148,544 - -0.1 148,937 + +0.6 149,318 + +0.0 149,527 + GFF1480 0.30 -1.0 149,527 + GFF1480 0.30 +0.2 149,665 + GFF1480 0.50 -0.2 149,666 - GFF1480 0.50 +1.7 149,735 + GFF1480 0.59 -0.6 149,751 + GFF1480 0.62 -0.4 149,853 + GFF1480 0.76 -0.1 149,855 + GFF1480 0.76 +0.4 149,856 - GFF1480 0.76 -0.5 149,856 - GFF1480 0.76 -0.1 149,970 - +0.2 150,013 + +0.5 150,060 + -0.2 150,265 + GFF1481 0.15 -0.7 150,271 - GFF1481 0.15 -0.1 150,360 - GFF1481 0.21 +0.1 150,381 - GFF1481 0.22 -1.1 150,381 - GFF1481 0.22 -0.4 150,406 - GFF1481 0.23 -0.3 150,515 - GFF1481 0.30 -0.6 150,518 + GFF1481 0.30 +0.0 150,519 - GFF1481 0.30 +0.2 150,536 + GFF1481 0.31 -0.0 150,538 + GFF1481 0.31 +1.4 150,540 - GFF1481 0.32 +0.4 150,555 - GFF1481 0.33 +1.1 150,556 + GFF1481 0.33 -0.1 150,557 - GFF1481 0.33 +0.2 150,557 - GFF1481 0.33 -0.1 150,592 - GFF1481 0.35 +0.0 150,637 + GFF1481 0.38 +0.7 150,687 - GFF1481 0.41 -0.1 150,743 + GFF1481 0.44 -0.4 150,818 + GFF1481 0.49 -0.4 150,915 - GFF1481 0.55 -0.0
Or see this region's nucleotide sequence