Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4675 and Atu4676 are separated by 67 nucleotides Atu4676 and Atu4677 are separated by 61 nucleotides Atu4677 and Atu4678 overlap by 4 nucleotides
Atu4675: Atu4675 - dihydrodipicolinate synthase, at 1,834,525 to 1,835,490
Atu4675
Atu4676: Atu4676 - malate dehydrogenase, at 1,835,558 to 1,836,595
Atu4676
Atu4677: Atu4677 - hypothetical protein, at 1,836,657 to 1,836,917
Atu4677
Atu4678: Atu4678 - amino acid ABC transporter substrate-binding protein, at 1,836,914 to 1,837,720
Atu4678
Position (kb)
1835
1836
1837 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1834.634 kb on + strand, within Atu4675 at 1834.694 kb on - strand, within Atu4675 at 1834.694 kb on - strand, within Atu4675 at 1834.733 kb on - strand, within Atu4675 at 1834.884 kb on + strand, within Atu4675 at 1834.884 kb on + strand, within Atu4675 at 1834.884 kb on + strand, within Atu4675 at 1834.961 kb on + strand, within Atu4675 at 1834.993 kb on + strand, within Atu4675 at 1835.086 kb on - strand, within Atu4675 at 1835.091 kb on - strand, within Atu4675 at 1835.167 kb on + strand, within Atu4675 at 1835.167 kb on + strand, within Atu4675 at 1835.329 kb on + strand, within Atu4675 at 1835.346 kb on + strand, within Atu4675 at 1835.423 kb on - strand at 1835.455 kb on - strand at 1835.475 kb on + strand at 1835.475 kb on + strand at 1835.547 kb on - strand at 1835.643 kb on + strand at 1835.683 kb on - strand, within Atu4676 at 1835.713 kb on + strand, within Atu4676 at 1835.721 kb on - strand, within Atu4676 at 1835.721 kb on - strand, within Atu4676 at 1835.833 kb on + strand, within Atu4676 at 1835.833 kb on + strand, within Atu4676 at 1835.841 kb on - strand, within Atu4676 at 1835.841 kb on - strand, within Atu4676 at 1835.921 kb on + strand, within Atu4676 at 1836.163 kb on - strand, within Atu4676 at 1836.202 kb on + strand, within Atu4676 at 1836.216 kb on + strand, within Atu4676 at 1836.347 kb on - strand, within Atu4676 at 1836.347 kb on - strand, within Atu4676 at 1836.395 kb on + strand, within Atu4676 at 1836.442 kb on + strand, within Atu4676 at 1836.509 kb on - strand at 1836.572 kb on + strand at 1836.580 kb on - strand at 1836.652 kb on + strand at 1836.700 kb on - strand, within Atu4677 at 1836.708 kb on + strand, within Atu4677 at 1836.970 kb on + strand at 1836.970 kb on + strand at 1837.236 kb on - strand, within Atu4678 at 1837.248 kb on - strand, within Atu4678 at 1837.324 kb on - strand, within Atu4678 at 1837.332 kb on + strand, within Atu4678 at 1837.372 kb on + strand, within Atu4678 at 1837.387 kb on + strand, within Atu4678 at 1837.413 kb on - strand, within Atu4678 at 1837.413 kb on - strand, within Atu4678 at 1837.413 kb on - strand, within Atu4678 at 1837.454 kb on + strand, within Atu4678 at 1837.472 kb on + strand, within Atu4678 at 1837.480 kb on - strand, within Atu4678 at 1837.499 kb on + strand, within Atu4678 at 1837.499 kb on + strand, within Atu4678 at 1837.499 kb on + strand, within Atu4678 at 1837.524 kb on - strand, within Atu4678 at 1837.547 kb on + strand, within Atu4678
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,834,634 + Atu4675 0.11 +0.6 1,834,694 - Atu4675 0.17 +0.0 1,834,694 - Atu4675 0.17 +0.4 1,834,733 - Atu4675 0.22 +0.1 1,834,884 + Atu4675 0.37 +1.0 1,834,884 + Atu4675 0.37 -1.1 1,834,884 + Atu4675 0.37 -0.5 1,834,961 + Atu4675 0.45 +0.6 1,834,993 + Atu4675 0.48 -2.0 1,835,086 - Atu4675 0.58 -1.2 1,835,091 - Atu4675 0.59 +1.9 1,835,167 + Atu4675 0.66 +0.7 1,835,167 + Atu4675 0.66 +1.1 1,835,329 + Atu4675 0.83 -0.1 1,835,346 + Atu4675 0.85 +0.7 1,835,423 - -0.2 1,835,455 - -0.1 1,835,475 + +1.4 1,835,475 + +0.3 1,835,547 - +0.5 1,835,643 + +2.2 1,835,683 - Atu4676 0.12 -1.1 1,835,713 + Atu4676 0.15 -0.6 1,835,721 - Atu4676 0.16 -0.0 1,835,721 - Atu4676 0.16 -2.5 1,835,833 + Atu4676 0.26 +0.1 1,835,833 + Atu4676 0.26 +1.2 1,835,841 - Atu4676 0.27 +0.8 1,835,841 - Atu4676 0.27 -0.2 1,835,921 + Atu4676 0.35 +2.1 1,836,163 - Atu4676 0.58 +0.2 1,836,202 + Atu4676 0.62 +0.4 1,836,216 + Atu4676 0.63 -1.0 1,836,347 - Atu4676 0.76 -0.1 1,836,347 - Atu4676 0.76 +0.4 1,836,395 + Atu4676 0.81 -1.9 1,836,442 + Atu4676 0.85 -0.9 1,836,509 - -0.4 1,836,572 + -0.1 1,836,580 - -0.7 1,836,652 + -0.4 1,836,700 - Atu4677 0.16 +2.0 1,836,708 + Atu4677 0.20 +0.4 1,836,970 + +0.5 1,836,970 + -0.2 1,837,236 - Atu4678 0.40 +0.2 1,837,248 - Atu4678 0.41 +0.5 1,837,324 - Atu4678 0.51 -0.2 1,837,332 + Atu4678 0.52 +2.8 1,837,372 + Atu4678 0.57 +2.3 1,837,387 + Atu4678 0.59 +1.1 1,837,413 - Atu4678 0.62 -0.1 1,837,413 - Atu4678 0.62 -0.1 1,837,413 - Atu4678 0.62 +0.3 1,837,454 + Atu4678 0.67 +0.6 1,837,472 + Atu4678 0.69 -0.2 1,837,480 - Atu4678 0.70 -0.6 1,837,499 + Atu4678 0.72 -0.5 1,837,499 + Atu4678 0.72 +0.1 1,837,499 + Atu4678 0.72 +0.3 1,837,524 - Atu4678 0.76 +0.0 1,837,547 + Atu4678 0.78 -0.5
Or see this region's nucleotide sequence