Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3707 and Atu3708 are separated by 213 nucleotides Atu3708 and Atu3709 are separated by 188 nucleotides
Atu3707: Atu3707 - phosphoserine aminotransferase, at 787,033 to 788,211
Atu3707
Atu3708: Atu3708 - outer surface protein, at 788,425 to 789,270
Atu3708
Atu3709: Atu3709 - phosphoglucosamine mutase, at 789,459 to 790,811
Atu3709
Position (kb) 788
789
790 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 787.454 kb on - strand, within Atu3707 at 787.719 kb on - strand, within Atu3707 at 787.833 kb on + strand, within Atu3707 at 787.965 kb on - strand, within Atu3707 at 788.239 kb on - strand at 788.371 kb on - strand at 788.423 kb on - strand at 788.470 kb on + strand at 788.478 kb on - strand at 788.556 kb on - strand, within Atu3708 at 788.629 kb on - strand, within Atu3708 at 788.629 kb on - strand, within Atu3708 at 788.636 kb on - strand, within Atu3708 at 788.656 kb on + strand, within Atu3708 at 788.752 kb on - strand, within Atu3708 at 788.752 kb on - strand, within Atu3708 at 788.754 kb on + strand, within Atu3708 at 788.834 kb on - strand, within Atu3708 at 788.839 kb on - strand, within Atu3708 at 788.893 kb on + strand, within Atu3708 at 788.901 kb on - strand, within Atu3708 at 789.137 kb on - strand, within Atu3708 at 789.142 kb on + strand, within Atu3708 at 789.155 kb on - strand, within Atu3708 at 789.157 kb on + strand, within Atu3708 at 789.168 kb on + strand, within Atu3708 at 789.316 kb on - strand at 789.323 kb on - strand at 789.437 kb on - strand at 789.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 787,454 - Atu3707 0.36 -1.1 787,719 - Atu3707 0.58 -3.1 787,833 + Atu3707 0.68 +1.1 787,965 - Atu3707 0.79 -1.5 788,239 - -0.3 788,371 - +0.7 788,423 - -0.1 788,470 + +1.5 788,478 - +1.6 788,556 - Atu3708 0.15 +1.9 788,629 - Atu3708 0.24 +2.6 788,629 - Atu3708 0.24 +1.6 788,636 - Atu3708 0.25 +1.2 788,656 + Atu3708 0.27 +2.2 788,752 - Atu3708 0.39 +1.4 788,752 - Atu3708 0.39 +2.6 788,754 + Atu3708 0.39 +1.6 788,834 - Atu3708 0.48 +2.4 788,839 - Atu3708 0.49 +2.9 788,893 + Atu3708 0.55 +2.8 788,901 - Atu3708 0.56 +2.0 789,137 - Atu3708 0.84 +1.9 789,142 + Atu3708 0.85 +2.2 789,155 - Atu3708 0.86 +2.0 789,157 + Atu3708 0.87 +1.7 789,168 + Atu3708 0.88 +3.0 789,316 - -0.2 789,323 - +0.2 789,437 - -0.2 789,458 - -1.3
Or see this region's nucleotide sequence