Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3511 and Atu3512 are separated by 12 nucleotides Atu3512 and Atu3513 are separated by 142 nucleotides Atu3513 and Atu3514 are separated by 17 nucleotides Atu3514 and Atu3515 are separated by 71 nucleotides Atu3515 and Atu3516 are separated by 172 nucleotides
Atu3511: Atu3511 - glutaredoxin, at 560,687 to 560,989
Atu3511
Atu3512: Atu3512 - competence protein F, at 561,002 to 561,793
Atu3512
Atu3513: Atu3513 - methyltransferase, at 561,936 to 562,817
Atu3513
Atu3514: Atu3514 - fimbriae associated protein, at 562,835 to 563,050
Atu3514
Atu3515: Atu3515 - hypothetical protein, at 563,122 to 563,436
Atu3515
Atu3516: Atu3516 - MutT family NTP pyrophosphatase, at 563,609 to 564,022
Atu3516
Position (kb) 561
562
563 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 560.945 kb on + strand, within Atu3511 at 560.953 kb on - strand, within Atu3511 at 561.040 kb on - strand at 561.188 kb on - strand, within Atu3512 at 561.291 kb on - strand, within Atu3512 at 561.296 kb on + strand, within Atu3512 at 561.371 kb on - strand, within Atu3512 at 561.382 kb on - strand, within Atu3512 at 561.433 kb on - strand, within Atu3512 at 561.509 kb on + strand, within Atu3512 at 561.581 kb on + strand, within Atu3512 at 561.581 kb on + strand, within Atu3512 at 561.581 kb on + strand, within Atu3512 at 561.581 kb on + strand, within Atu3512 at 561.589 kb on - strand, within Atu3512 at 561.589 kb on - strand, within Atu3512 at 561.589 kb on - strand, within Atu3512 at 561.589 kb on - strand, within Atu3512 at 561.589 kb on - strand, within Atu3512 at 561.589 kb on - strand, within Atu3512 at 561.589 kb on - strand, within Atu3512 at 561.686 kb on - strand, within Atu3512 at 561.686 kb on - strand, within Atu3512 at 561.758 kb on - strand at 561.760 kb on - strand at 561.839 kb on + strand at 561.912 kb on + strand at 562.787 kb on - strand at 562.887 kb on + strand, within Atu3514 at 563.140 kb on + strand at 563.148 kb on - strand at 563.148 kb on - strand at 563.169 kb on - strand, within Atu3515 at 563.209 kb on - strand, within Atu3515 at 563.215 kb on - strand, within Atu3515 at 563.215 kb on - strand, within Atu3515 at 563.301 kb on + strand, within Atu3515 at 563.301 kb on + strand, within Atu3515 at 563.538 kb on - strand at 563.579 kb on + strand at 563.586 kb on - strand at 563.626 kb on + strand at 563.636 kb on - strand at 563.676 kb on + strand, within Atu3516 at 563.736 kb on - strand, within Atu3516 at 563.780 kb on + strand, within Atu3516 at 563.811 kb on - strand, within Atu3516
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 560,945 + Atu3511 0.85 -0.1 560,953 - Atu3511 0.88 -0.0 561,040 - -0.9 561,188 - Atu3512 0.23 -0.8 561,291 - Atu3512 0.36 -0.5 561,296 + Atu3512 0.37 -0.6 561,371 - Atu3512 0.47 -1.4 561,382 - Atu3512 0.48 +0.8 561,433 - Atu3512 0.54 -0.1 561,509 + Atu3512 0.64 -0.4 561,581 + Atu3512 0.73 +0.5 561,581 + Atu3512 0.73 -0.2 561,581 + Atu3512 0.73 -0.0 561,581 + Atu3512 0.73 +2.0 561,589 - Atu3512 0.74 -1.5 561,589 - Atu3512 0.74 +0.4 561,589 - Atu3512 0.74 +1.0 561,589 - Atu3512 0.74 -1.9 561,589 - Atu3512 0.74 -0.2 561,589 - Atu3512 0.74 +1.6 561,589 - Atu3512 0.74 +0.8 561,686 - Atu3512 0.86 -0.8 561,686 - Atu3512 0.86 -0.4 561,758 - -0.1 561,760 - -1.2 561,839 + -1.0 561,912 + -0.1 562,787 - +0.3 562,887 + Atu3514 0.24 -0.1 563,140 + -2.4 563,148 - -2.6 563,148 - -1.7 563,169 - Atu3515 0.15 -1.1 563,209 - Atu3515 0.28 -0.2 563,215 - Atu3515 0.30 -2.5 563,215 - Atu3515 0.30 +0.3 563,301 + Atu3515 0.57 -1.2 563,301 + Atu3515 0.57 -3.1 563,538 - -0.2 563,579 + +0.3 563,586 - +0.2 563,626 + -0.1 563,636 - +0.3 563,676 + Atu3516 0.16 -0.3 563,736 - Atu3516 0.31 -2.0 563,780 + Atu3516 0.41 +0.2 563,811 - Atu3516 0.49 -0.5
Or see this region's nucleotide sequence