Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3385 and Atu3386 are separated by 149 nucleotides Atu3386 and Atu3387 are separated by 111 nucleotides Atu3387 and Atu3388 are separated by 211 nucleotides
Atu3385: Atu3385 - heme receptor, at 427,642 to 430,413
Atu3385
Atu3386: Atu3386 - two component sensor kinase, at 430,563 to 431,558
Atu3386
Atu3387: Atu3387 - sigma factor, at 431,670 to 432,170
Atu3387
Atu3388: Atu3388 - iron ABC transporter ATPase, at 432,382 to 433,134
Atu3388
Position (kb)
430
431
432 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 429.631 kb on - strand, within Atu3385 at 429.751 kb on + strand, within Atu3385 at 429.905 kb on - strand, within Atu3385 at 430.009 kb on + strand, within Atu3385 at 430.032 kb on + strand, within Atu3385 at 430.040 kb on - strand, within Atu3385 at 430.040 kb on - strand, within Atu3385 at 430.129 kb on + strand, within Atu3385 at 430.297 kb on + strand at 430.569 kb on - strand at 430.640 kb on - strand at 430.651 kb on + strand at 430.654 kb on - strand at 430.710 kb on + strand, within Atu3386 at 430.732 kb on + strand, within Atu3386 at 430.732 kb on + strand, within Atu3386 at 430.740 kb on - strand, within Atu3386 at 430.775 kb on - strand, within Atu3386 at 430.826 kb on - strand, within Atu3386 at 430.917 kb on + strand, within Atu3386 at 431.058 kb on + strand, within Atu3386 at 431.074 kb on - strand, within Atu3386 at 431.134 kb on - strand, within Atu3386 at 431.188 kb on + strand, within Atu3386 at 431.225 kb on + strand, within Atu3386 at 431.225 kb on + strand, within Atu3386 at 431.225 kb on + strand, within Atu3386 at 431.225 kb on + strand, within Atu3386 at 431.225 kb on + strand, within Atu3386 at 431.233 kb on - strand, within Atu3386 at 431.233 kb on - strand, within Atu3386 at 431.481 kb on - strand at 431.556 kb on + strand at 431.657 kb on - strand at 431.823 kb on + strand, within Atu3387 at 432.058 kb on + strand, within Atu3387 at 432.077 kb on + strand, within Atu3387 at 432.089 kb on + strand, within Atu3387 at 432.121 kb on - strand at 432.121 kb on - strand at 432.121 kb on - strand at 432.178 kb on - strand at 432.217 kb on - strand at 432.232 kb on + strand at 432.240 kb on - strand at 432.317 kb on + strand at 432.317 kb on + strand at 432.405 kb on + strand at 432.413 kb on - strand at 432.423 kb on - strand at 432.455 kb on + strand at 432.552 kb on + strand, within Atu3388
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 429,631 - Atu3385 0.72 -0.2 429,751 + Atu3385 0.76 -0.2 429,905 - Atu3385 0.82 -0.2 430,009 + Atu3385 0.85 +0.7 430,032 + Atu3385 0.86 +0.9 430,040 - Atu3385 0.87 -0.9 430,040 - Atu3385 0.87 +1.5 430,129 + Atu3385 0.90 -1.3 430,297 + -0.1 430,569 - -0.6 430,640 - +0.4 430,651 + -0.0 430,654 - +0.5 430,710 + Atu3386 0.15 -0.7 430,732 + Atu3386 0.17 -1.6 430,732 + Atu3386 0.17 -0.1 430,740 - Atu3386 0.18 -1.2 430,775 - Atu3386 0.21 -1.6 430,826 - Atu3386 0.26 +1.3 430,917 + Atu3386 0.36 +0.8 431,058 + Atu3386 0.50 -0.2 431,074 - Atu3386 0.51 -0.0 431,134 - Atu3386 0.57 -1.3 431,188 + Atu3386 0.63 -1.0 431,225 + Atu3386 0.66 -0.1 431,225 + Atu3386 0.66 +0.2 431,225 + Atu3386 0.66 +2.3 431,225 + Atu3386 0.66 +0.0 431,225 + Atu3386 0.66 -1.8 431,233 - Atu3386 0.67 -1.0 431,233 - Atu3386 0.67 -1.7 431,481 - +0.9 431,556 + +0.3 431,657 - -0.2 431,823 + Atu3387 0.31 -0.2 432,058 + Atu3387 0.77 -1.7 432,077 + Atu3387 0.81 +1.3 432,089 + Atu3387 0.84 -1.5 432,121 - +1.7 432,121 - +0.8 432,121 - -0.2 432,178 - +1.8 432,217 - +1.7 432,232 + -0.8 432,240 - -1.4 432,317 + -0.9 432,317 + +2.5 432,405 + -0.1 432,413 - -0.4 432,423 - -0.9 432,455 + -2.8 432,552 + Atu3388 0.23 -0.6
Or see this region's nucleotide sequence