Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2021 and Atu2022 are separated by 189 nucleotides Atu2022 and Atu2023 are separated by 66 nucleotides
Atu2021: Atu2021 - transcriptional regulator, AraC family, at 1,982,112 to 1,983,017
Atu2021
Atu2022: Atu2022 - NADP-dependent alcohol dehydrogenase, at 1,983,207 to 1,984,253
Atu2022
Atu2023: Atu2023 - NADH dehydrogenase, at 1,984,320 to 1,985,585
Atu2023
Position (kb)
1983
1984
1985 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1982.396 kb on - strand, within Atu2021 at 1982.611 kb on - strand, within Atu2021 at 1982.613 kb on + strand, within Atu2021 at 1982.621 kb on - strand, within Atu2021 at 1982.719 kb on - strand, within Atu2021 at 1982.893 kb on + strand, within Atu2021 at 1982.938 kb on + strand at 1983.152 kb on + strand at 1983.221 kb on + strand at 1983.223 kb on + strand at 1983.229 kb on - strand at 1983.258 kb on - strand at 1983.338 kb on + strand, within Atu2022 at 1983.346 kb on - strand, within Atu2022 at 1983.440 kb on + strand, within Atu2022 at 1983.440 kb on + strand, within Atu2022 at 1983.448 kb on - strand, within Atu2022 at 1983.448 kb on - strand, within Atu2022 at 1983.448 kb on - strand, within Atu2022 at 1983.514 kb on + strand, within Atu2022 at 1983.611 kb on + strand, within Atu2022 at 1983.704 kb on - strand, within Atu2022 at 1983.723 kb on - strand, within Atu2022 at 1983.773 kb on - strand, within Atu2022 at 1983.847 kb on + strand, within Atu2022 at 1983.922 kb on + strand, within Atu2022 at 1983.967 kb on + strand, within Atu2022 at 1983.967 kb on + strand, within Atu2022 at 1984.007 kb on - strand, within Atu2022 at 1984.013 kb on + strand, within Atu2022 at 1984.182 kb on + strand at 1984.182 kb on + strand at 1984.182 kb on + strand at 1984.332 kb on - strand at 1984.338 kb on - strand at 1984.382 kb on + strand at 1984.390 kb on - strand at 1984.494 kb on - strand, within Atu2023 at 1984.797 kb on - strand, within Atu2023 at 1984.805 kb on - strand, within Atu2023 at 1984.805 kb on - strand, within Atu2023 at 1984.835 kb on + strand, within Atu2023 at 1984.835 kb on + strand, within Atu2023 at 1984.863 kb on - strand, within Atu2023 at 1984.893 kb on + strand, within Atu2023 at 1984.896 kb on - strand, within Atu2023 at 1984.946 kb on - strand, within Atu2023 at 1985.010 kb on + strand, within Atu2023 at 1985.018 kb on - strand, within Atu2023 at 1985.025 kb on - strand, within Atu2023 at 1985.157 kb on - strand, within Atu2023
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,982,396 - Atu2021 0.31 +1.4 1,982,611 - Atu2021 0.55 -1.2 1,982,613 + Atu2021 0.55 -0.2 1,982,621 - Atu2021 0.56 +0.0 1,982,719 - Atu2021 0.67 -1.2 1,982,893 + Atu2021 0.86 +0.2 1,982,938 + +0.2 1,983,152 + +0.5 1,983,221 + -0.9 1,983,223 + +0.2 1,983,229 - +1.0 1,983,258 - -1.5 1,983,338 + Atu2022 0.13 -0.3 1,983,346 - Atu2022 0.13 -0.6 1,983,440 + Atu2022 0.22 +0.4 1,983,440 + Atu2022 0.22 -0.3 1,983,448 - Atu2022 0.23 -0.2 1,983,448 - Atu2022 0.23 -0.3 1,983,448 - Atu2022 0.23 +0.7 1,983,514 + Atu2022 0.29 +0.3 1,983,611 + Atu2022 0.39 -3.1 1,983,704 - Atu2022 0.47 -0.4 1,983,723 - Atu2022 0.49 -0.5 1,983,773 - Atu2022 0.54 -0.7 1,983,847 + Atu2022 0.61 +1.0 1,983,922 + Atu2022 0.68 +0.4 1,983,967 + Atu2022 0.73 -1.2 1,983,967 + Atu2022 0.73 -1.6 1,984,007 - Atu2022 0.76 +0.8 1,984,013 + Atu2022 0.77 +0.8 1,984,182 + +1.2 1,984,182 + +0.6 1,984,182 + -1.0 1,984,332 - -0.7 1,984,338 - +0.4 1,984,382 + -1.1 1,984,390 - +0.3 1,984,494 - Atu2023 0.14 +0.9 1,984,797 - Atu2023 0.38 -0.9 1,984,805 - Atu2023 0.38 +0.7 1,984,805 - Atu2023 0.38 -1.2 1,984,835 + Atu2023 0.41 -1.3 1,984,835 + Atu2023 0.41 -1.6 1,984,863 - Atu2023 0.43 -0.9 1,984,893 + Atu2023 0.45 +0.8 1,984,896 - Atu2023 0.45 -1.5 1,984,946 - Atu2023 0.49 -1.6 1,985,010 + Atu2023 0.55 -0.5 1,985,018 - Atu2023 0.55 +1.1 1,985,025 - Atu2023 0.56 -1.8 1,985,157 - Atu2023 0.66 +0.1
Or see this region's nucleotide sequence