Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu2003 and Atu2004 are separated by 141 nucleotides Atu2004 and Atu2005 are separated by 188 nucleotides Atu2005 and Atu2006 are separated by 10 nucleotides Atu2006 and Atu8168 are separated by 384 nucleotides
Atu2003: Atu2003 - hypothetical protein, at 1,966,889 to 1,967,314
Atu2003
Atu2004: Atu2004 - hypothetical protein, at 1,967,456 to 1,967,911
Atu2004
Atu2005: Atu2005 - hypothetical protein, at 1,968,100 to 1,968,708
Atu2005
Atu2006: Atu2006 - molecular chaperone, DnaJ family, at 1,968,719 to 1,969,321
Atu2006
Atu8168: Atu8168 - hypothetical protein, at 1,969,706 to 1,970,059
Atu8168
Position (kb)
1968
1969 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1967.102 kb on + strand, within Atu2003 at 1967.102 kb on + strand, within Atu2003 at 1967.110 kb on - strand, within Atu2003 at 1967.127 kb on - strand, within Atu2003 at 1967.127 kb on - strand, within Atu2003 at 1967.199 kb on - strand, within Atu2003 at 1967.338 kb on + strand at 1967.492 kb on + strand at 1967.628 kb on + strand, within Atu2004 at 1967.658 kb on - strand, within Atu2004 at 1967.673 kb on - strand, within Atu2004 at 1967.751 kb on + strand, within Atu2004 at 1967.813 kb on + strand, within Atu2004 at 1967.867 kb on + strand at 1967.917 kb on - strand at 1968.016 kb on - strand at 1968.025 kb on - strand at 1968.041 kb on - strand at 1968.144 kb on + strand at 1968.201 kb on + strand, within Atu2005 at 1968.209 kb on - strand, within Atu2005 at 1968.239 kb on + strand, within Atu2005 at 1968.247 kb on - strand, within Atu2005 at 1968.347 kb on - strand, within Atu2005 at 1968.363 kb on - strand, within Atu2005 at 1968.371 kb on + strand, within Atu2005 at 1968.387 kb on + strand, within Atu2005 at 1968.396 kb on - strand, within Atu2005 at 1968.396 kb on - strand, within Atu2005 at 1968.406 kb on - strand, within Atu2005 at 1968.470 kb on + strand, within Atu2005 at 1968.525 kb on + strand, within Atu2005 at 1968.525 kb on + strand, within Atu2005 at 1968.525 kb on + strand, within Atu2005 at 1968.531 kb on - strand, within Atu2005 at 1968.611 kb on - strand, within Atu2005 at 1968.724 kb on - strand at 1968.780 kb on + strand, within Atu2006 at 1968.798 kb on + strand, within Atu2006 at 1968.798 kb on + strand, within Atu2006 at 1968.798 kb on + strand, within Atu2006 at 1968.798 kb on + strand, within Atu2006 at 1968.806 kb on - strand, within Atu2006 at 1968.806 kb on - strand, within Atu2006 at 1968.806 kb on - strand, within Atu2006 at 1968.806 kb on - strand, within Atu2006 at 1968.877 kb on + strand, within Atu2006 at 1968.885 kb on - strand, within Atu2006 at 1968.920 kb on + strand, within Atu2006 at 1969.056 kb on - strand, within Atu2006 at 1969.139 kb on + strand, within Atu2006 at 1969.155 kb on - strand, within Atu2006 at 1969.231 kb on - strand, within Atu2006 at 1969.236 kb on + strand, within Atu2006 at 1969.371 kb on + strand at 1969.379 kb on - strand at 1969.408 kb on - strand at 1969.412 kb on + strand at 1969.456 kb on + strand at 1969.597 kb on + strand at 1969.609 kb on + strand at 1969.627 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,967,102 + Atu2003 0.50 -1.4 1,967,102 + Atu2003 0.50 -0.0 1,967,110 - Atu2003 0.52 -0.2 1,967,127 - Atu2003 0.56 -0.3 1,967,127 - Atu2003 0.56 -0.7 1,967,199 - Atu2003 0.73 -0.4 1,967,338 + +0.5 1,967,492 + -0.6 1,967,628 + Atu2004 0.38 +0.6 1,967,658 - Atu2004 0.44 +0.8 1,967,673 - Atu2004 0.48 -0.4 1,967,751 + Atu2004 0.65 -0.0 1,967,813 + Atu2004 0.78 -0.3 1,967,867 + -0.4 1,967,917 - +0.3 1,968,016 - +0.1 1,968,025 - -0.6 1,968,041 - -0.1 1,968,144 + -2.3 1,968,201 + Atu2005 0.17 -2.6 1,968,209 - Atu2005 0.18 -2.3 1,968,239 + Atu2005 0.23 -1.3 1,968,247 - Atu2005 0.24 -0.9 1,968,347 - Atu2005 0.41 -2.7 1,968,363 - Atu2005 0.43 -1.8 1,968,371 + Atu2005 0.44 -1.7 1,968,387 + Atu2005 0.47 -3.2 1,968,396 - Atu2005 0.49 +0.0 1,968,396 - Atu2005 0.49 -1.6 1,968,406 - Atu2005 0.50 -0.1 1,968,470 + Atu2005 0.61 -1.4 1,968,525 + Atu2005 0.70 -0.3 1,968,525 + Atu2005 0.70 -2.0 1,968,525 + Atu2005 0.70 -1.8 1,968,531 - Atu2005 0.71 -1.7 1,968,611 - Atu2005 0.84 -1.9 1,968,724 - -0.5 1,968,780 + Atu2006 0.10 -1.0 1,968,798 + Atu2006 0.13 -1.3 1,968,798 + Atu2006 0.13 +0.8 1,968,798 + Atu2006 0.13 +1.7 1,968,798 + Atu2006 0.13 -0.4 1,968,806 - Atu2006 0.14 -0.7 1,968,806 - Atu2006 0.14 +1.2 1,968,806 - Atu2006 0.14 -0.1 1,968,806 - Atu2006 0.14 -0.3 1,968,877 + Atu2006 0.26 +0.3 1,968,885 - Atu2006 0.28 -0.8 1,968,920 + Atu2006 0.33 +0.3 1,969,056 - Atu2006 0.56 +1.4 1,969,139 + Atu2006 0.70 -0.3 1,969,155 - Atu2006 0.72 +0.2 1,969,231 - Atu2006 0.85 -0.1 1,969,236 + Atu2006 0.86 +0.7 1,969,371 + -2.4 1,969,379 - +0.3 1,969,408 - -0.5 1,969,412 + +0.2 1,969,456 + +0.3 1,969,597 + -0.1 1,969,609 + +1.6 1,969,627 - +0.7
Or see this region's nucleotide sequence