Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1868 and Atu1869 are separated by 114 nucleotides Atu1869 and Atu1870 are separated by 227 nucleotides Atu1870 and Atu1871 are separated by 335 nucleotides
Atu1868: Atu1868 - tRNA/rRNA methyltransferase, at 1,846,590 to 1,847,423
Atu1868
Atu1869: Atu1869 - hypothetical protein, at 1,847,538 to 1,848,056
Atu1869
Atu1870: Atu1870 - isocitrate dehydrogenase, at 1,848,284 to 1,849,498
Atu1870
Atu1871: Atu1871 - glutathione S-transferase, at 1,849,834 to 1,850,460
Atu1871
Position (kb)
1848
1849
1850 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1847.364 kb on + strand at 1847.407 kb on + strand at 1847.445 kb on - strand at 1847.477 kb on - strand at 1847.488 kb on - strand at 1847.490 kb on + strand at 1847.490 kb on + strand at 1847.498 kb on - strand at 1847.531 kb on - strand at 1847.581 kb on + strand at 1847.668 kb on - strand, within Atu1869 at 1847.686 kb on - strand, within Atu1869 at 1847.697 kb on + strand, within Atu1869 at 1847.951 kb on + strand, within Atu1869 at 1847.969 kb on - strand, within Atu1869 at 1847.969 kb on - strand, within Atu1869 at 1848.081 kb on + strand at 1848.156 kb on + strand at 1848.190 kb on - strand at 1848.212 kb on + strand at 1849.508 kb on + strand at 1849.542 kb on + strand at 1849.617 kb on - strand at 1849.641 kb on - strand at 1849.900 kb on + strand, within Atu1871 at 1850.060 kb on - strand, within Atu1871 at 1850.087 kb on + strand, within Atu1871 at 1850.171 kb on + strand, within Atu1871 at 1850.186 kb on + strand, within Atu1871 at 1850.229 kb on + strand, within Atu1871 at 1850.326 kb on + strand, within Atu1871 at 1850.344 kb on + strand, within Atu1871 at 1850.352 kb on - strand, within Atu1871 at 1850.473 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,847,364 + -0.7 1,847,407 + -0.4 1,847,445 - -0.2 1,847,477 - -1.3 1,847,488 - -0.0 1,847,490 + -1.6 1,847,490 + -1.2 1,847,498 - -0.0 1,847,531 - -0.9 1,847,581 + -0.5 1,847,668 - Atu1869 0.25 -1.0 1,847,686 - Atu1869 0.29 -1.3 1,847,697 + Atu1869 0.31 +0.9 1,847,951 + Atu1869 0.80 -0.8 1,847,969 - Atu1869 0.83 -0.5 1,847,969 - Atu1869 0.83 -0.0 1,848,081 + -0.4 1,848,156 + -1.7 1,848,190 - -0.6 1,848,212 + -2.0 1,849,508 + +2.4 1,849,542 + +0.4 1,849,617 - -1.0 1,849,641 - -0.3 1,849,900 + Atu1871 0.11 -1.3 1,850,060 - Atu1871 0.36 -0.5 1,850,087 + Atu1871 0.40 +0.6 1,850,171 + Atu1871 0.54 +1.2 1,850,186 + Atu1871 0.56 -1.2 1,850,229 + Atu1871 0.63 +1.5 1,850,326 + Atu1871 0.78 -3.6 1,850,344 + Atu1871 0.81 -0.2 1,850,352 - Atu1871 0.83 -0.1 1,850,473 + +0.9
Or see this region's nucleotide sequence