Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1518 and Atu1519 overlap by 4 nucleotides Atu1519 and Atu1520 overlap by 8 nucleotides Atu1520 and Atu1521 are separated by 104 nucleotides
Atu1518: Atu1518 - transcriptional regulator, Fur family, at 1,508,616 to 1,509,029
Atu1518
Atu1519: Atu1519 - ABC transporter, membrane spanning protein (zinc), at 1,509,026 to 1,509,844
Atu1519
Atu1520: Atu1520 - ABC transporter, nucleotide binding/ATPase protein (zinc), at 1,509,837 to 1,510,736
Atu1520
Atu1521: Atu1521 - ABC transporter, substrate binding protein (zinc), at 1,510,841 to 1,511,830
Atu1521
Position (kb)
1509
1510
1511 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1508.854 kb on + strand, within Atu1518 at 1508.936 kb on + strand, within Atu1518 at 1508.952 kb on - strand, within Atu1518 at 1508.962 kb on + strand, within Atu1518 at 1508.977 kb on + strand, within Atu1518 at 1509.087 kb on - strand at 1509.130 kb on + strand, within Atu1519 at 1509.135 kb on + strand, within Atu1519 at 1509.138 kb on - strand, within Atu1519 at 1509.216 kb on - strand, within Atu1519 at 1509.305 kb on + strand, within Atu1519 at 1509.313 kb on - strand, within Atu1519 at 1509.371 kb on - strand, within Atu1519 at 1509.371 kb on - strand, within Atu1519 at 1509.447 kb on + strand, within Atu1519 at 1509.447 kb on + strand, within Atu1519 at 1509.519 kb on - strand, within Atu1519 at 1509.587 kb on - strand, within Atu1519 at 1509.635 kb on + strand, within Atu1519 at 1509.643 kb on - strand, within Atu1519 at 1509.789 kb on - strand at 1509.968 kb on - strand, within Atu1520 at 1510.118 kb on + strand, within Atu1520 at 1510.118 kb on + strand, within Atu1520 at 1510.325 kb on - strand, within Atu1520 at 1510.325 kb on - strand, within Atu1520 at 1510.325 kb on - strand, within Atu1520 at 1510.340 kb on - strand, within Atu1520 at 1510.371 kb on + strand, within Atu1520 at 1510.384 kb on - strand, within Atu1520 at 1510.674 kb on - strand at 1510.708 kb on + strand at 1510.716 kb on + strand at 1510.716 kb on + strand at 1510.724 kb on - strand at 1510.726 kb on + strand at 1510.827 kb on + strand at 1510.940 kb on + strand, within Atu1521 at 1510.971 kb on - strand, within Atu1521 at 1510.990 kb on - strand, within Atu1521 at 1511.027 kb on - strand, within Atu1521 at 1511.027 kb on - strand, within Atu1521 at 1511.082 kb on + strand, within Atu1521 at 1511.125 kb on + strand, within Atu1521 at 1511.200 kb on + strand, within Atu1521 at 1511.220 kb on - strand, within Atu1521 at 1511.295 kb on + strand, within Atu1521 at 1511.332 kb on + strand, within Atu1521 at 1511.713 kb on - strand, within Atu1521
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,508,854 + Atu1518 0.57 +1.1 1,508,936 + Atu1518 0.77 +1.6 1,508,952 - Atu1518 0.81 +2.1 1,508,962 + Atu1518 0.84 +0.1 1,508,977 + Atu1518 0.87 +1.9 1,509,087 - +0.7 1,509,130 + Atu1519 0.13 +0.5 1,509,135 + Atu1519 0.13 +1.1 1,509,138 - Atu1519 0.14 +0.4 1,509,216 - Atu1519 0.23 -1.1 1,509,305 + Atu1519 0.34 -0.3 1,509,313 - Atu1519 0.35 -0.1 1,509,371 - Atu1519 0.42 +0.1 1,509,371 - Atu1519 0.42 +1.8 1,509,447 + Atu1519 0.51 +2.0 1,509,447 + Atu1519 0.51 -0.5 1,509,519 - Atu1519 0.60 +0.4 1,509,587 - Atu1519 0.68 -0.6 1,509,635 + Atu1519 0.74 +0.1 1,509,643 - Atu1519 0.75 +0.6 1,509,789 - +0.2 1,509,968 - Atu1520 0.15 +0.6 1,510,118 + Atu1520 0.31 +1.8 1,510,118 + Atu1520 0.31 -1.3 1,510,325 - Atu1520 0.54 +1.2 1,510,325 - Atu1520 0.54 +0.6 1,510,325 - Atu1520 0.54 +0.6 1,510,340 - Atu1520 0.56 +0.7 1,510,371 + Atu1520 0.59 -0.0 1,510,384 - Atu1520 0.61 -0.1 1,510,674 - +0.0 1,510,708 + -0.7 1,510,716 + +0.2 1,510,716 + -1.1 1,510,724 - +0.5 1,510,726 + +1.0 1,510,827 + +3.2 1,510,940 + Atu1521 0.10 +1.5 1,510,971 - Atu1521 0.13 -2.7 1,510,990 - Atu1521 0.15 +1.3 1,511,027 - Atu1521 0.19 +0.3 1,511,027 - Atu1521 0.19 +0.6 1,511,082 + Atu1521 0.24 -0.9 1,511,125 + Atu1521 0.29 -0.4 1,511,200 + Atu1521 0.36 +1.6 1,511,220 - Atu1521 0.38 +1.0 1,511,295 + Atu1521 0.46 -0.3 1,511,332 + Atu1521 0.50 +0.3 1,511,713 - Atu1521 0.88 +0.8
Or see this region's nucleotide sequence