Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1426 and Atu1427 are separated by 12 nucleotides Atu1427 and Atu1428 are separated by 121 nucleotides Atu1428 and Atu1429 are separated by 276 nucleotides Atu1429 and Atu1430 are separated by 17 nucleotides
Atu1426: Atu1426 - enolase, at 1,421,590 to 1,422,864
Atu1426
Atu1427: Atu1427 - hypothetical protein, at 1,422,877 to 1,423,056
Atu1427
Atu1428: Atu1428 - hypothetical protein, at 1,423,178 to 1,423,495
Atu1428
Atu1429: Atu1429 - pyruvate dehydrogenase alpha subunit, at 1,423,772 to 1,424,692
Atu1429
Atu1430: Atu1430 - pyruvate dehydrogenase beta subunit, at 1,424,710 to 1,426,131
Atu1430
Position (kb)
1423
1424
1425 Strain fitness (log2 ratio)
-1
0
1
2 at 1422.921 kb on + strand, within Atu1427 at 1422.921 kb on + strand, within Atu1427 at 1422.947 kb on + strand, within Atu1427 at 1422.947 kb on + strand, within Atu1427 at 1423.037 kb on - strand, within Atu1427 at 1423.051 kb on + strand at 1423.059 kb on - strand at 1423.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 1,422,921 + Atu1427 0.24 -0.7 1,422,921 + Atu1427 0.24 -0.2 1,422,947 + Atu1427 0.39 -0.0 1,422,947 + Atu1427 0.39 -1.0 1,423,037 - Atu1427 0.89 +0.3 1,423,051 + +0.2 1,423,059 - +0.6 1,423,145 + +1.9
Or see this region's nucleotide sequence