Experiment: R2A with Vancomycin 0.009 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0302 and Atu0303 overlap by 11 nucleotides Atu0303 and Atu0304 are separated by 16 nucleotides Atu0304 and Atu0305 are separated by 108 nucleotides
Atu0302: Atu0302 - tetrapyrrole methylase family protein, at 295,140 to 296,081
Atu0302
Atu0303: Atu0303 - hypothetical protein, at 296,071 to 296,439
Atu0303
Atu0304: Atu0304 - nodulation protein L, at 296,456 to 297,016
Atu0304
Atu0305: Atu0305 - ABC transporter, substrate binding protein (sn-glycerol 3-phosphate), at 297,125 to 298,423
Atu0305
Position (kb) 296
297 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 295.232 kb on - strand at 295.274 kb on - strand, within Atu0302 at 295.415 kb on - strand, within Atu0302 at 295.431 kb on + strand, within Atu0302 at 295.440 kb on - strand, within Atu0302 at 295.447 kb on - strand, within Atu0302 at 295.545 kb on + strand, within Atu0302 at 295.547 kb on - strand, within Atu0302 at 295.566 kb on + strand, within Atu0302 at 295.576 kb on + strand, within Atu0302 at 295.576 kb on + strand, within Atu0302 at 295.584 kb on - strand, within Atu0302 at 295.584 kb on - strand, within Atu0302 at 295.603 kb on + strand, within Atu0302 at 295.631 kb on - strand, within Atu0302 at 295.699 kb on + strand, within Atu0302 at 295.712 kb on - strand, within Atu0302 at 295.756 kb on + strand, within Atu0302 at 295.756 kb on + strand, within Atu0302 at 295.795 kb on + strand, within Atu0302 at 295.803 kb on - strand, within Atu0302 at 295.803 kb on - strand, within Atu0302 at 295.896 kb on - strand, within Atu0302 at 296.012 kb on + strand at 296.012 kb on + strand at 296.020 kb on - strand at 296.108 kb on - strand, within Atu0303 at 296.133 kb on + strand, within Atu0303 at 296.144 kb on + strand, within Atu0303 at 296.152 kb on - strand, within Atu0303 at 296.157 kb on + strand, within Atu0303 at 296.335 kb on - strand, within Atu0303 at 296.492 kb on + strand at 296.502 kb on - strand at 296.525 kb on - strand, within Atu0304 at 296.557 kb on + strand, within Atu0304 at 296.557 kb on + strand, within Atu0304 at 296.565 kb on - strand, within Atu0304 at 296.569 kb on + strand, within Atu0304 at 296.676 kb on + strand, within Atu0304 at 296.676 kb on + strand, within Atu0304 at 296.684 kb on - strand, within Atu0304 at 296.728 kb on - strand, within Atu0304 at 296.745 kb on - strand, within Atu0304 at 296.774 kb on - strand, within Atu0304 at 296.779 kb on + strand, within Atu0304 at 296.779 kb on + strand, within Atu0304 at 296.782 kb on - strand, within Atu0304 at 296.787 kb on - strand, within Atu0304 at 296.805 kb on + strand, within Atu0304 at 296.856 kb on + strand, within Atu0304 at 296.872 kb on - strand, within Atu0304 at 296.914 kb on + strand, within Atu0304 at 296.924 kb on - strand, within Atu0304 at 296.924 kb on - strand, within Atu0304 at 296.954 kb on + strand, within Atu0304 at 296.954 kb on + strand, within Atu0304 at 296.958 kb on - strand, within Atu0304 at 296.973 kb on - strand at 297.086 kb on - strand at 297.186 kb on - strand at 297.188 kb on - strand at 297.213 kb on + strand at 297.213 kb on + strand at 297.221 kb on - strand at 297.251 kb on + strand at 297.269 kb on + strand, within Atu0305 at 297.277 kb on - strand, within Atu0305 at 297.333 kb on - strand, within Atu0305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.009 mM remove 295,232 - -2.0 295,274 - Atu0302 0.14 -1.2 295,415 - Atu0302 0.29 -2.1 295,431 + Atu0302 0.31 -0.6 295,440 - Atu0302 0.32 -3.1 295,447 - Atu0302 0.33 -2.9 295,545 + Atu0302 0.43 -1.3 295,547 - Atu0302 0.43 -3.6 295,566 + Atu0302 0.45 -1.5 295,576 + Atu0302 0.46 -2.5 295,576 + Atu0302 0.46 -0.6 295,584 - Atu0302 0.47 +0.7 295,584 - Atu0302 0.47 -1.1 295,603 + Atu0302 0.49 -4.0 295,631 - Atu0302 0.52 -2.5 295,699 + Atu0302 0.59 -1.2 295,712 - Atu0302 0.61 -0.8 295,756 + Atu0302 0.65 -1.9 295,756 + Atu0302 0.65 -1.9 295,795 + Atu0302 0.70 -1.7 295,803 - Atu0302 0.70 +0.1 295,803 - Atu0302 0.70 -0.8 295,896 - Atu0302 0.80 -1.7 296,012 + -3.5 296,012 + -0.9 296,020 - -1.0 296,108 - Atu0303 0.10 -0.1 296,133 + Atu0303 0.17 -0.1 296,144 + Atu0303 0.20 -0.4 296,152 - Atu0303 0.22 -0.4 296,157 + Atu0303 0.23 -0.1 296,335 - Atu0303 0.72 -0.9 296,492 + +2.0 296,502 - +2.2 296,525 - Atu0304 0.12 +0.5 296,557 + Atu0304 0.18 +0.5 296,557 + Atu0304 0.18 +0.7 296,565 - Atu0304 0.19 +1.0 296,569 + Atu0304 0.20 +0.5 296,676 + Atu0304 0.39 -0.8 296,676 + Atu0304 0.39 +0.1 296,684 - Atu0304 0.41 +0.5 296,728 - Atu0304 0.48 -0.5 296,745 - Atu0304 0.52 -0.2 296,774 - Atu0304 0.57 +0.0 296,779 + Atu0304 0.58 -0.8 296,779 + Atu0304 0.58 -0.9 296,782 - Atu0304 0.58 -1.2 296,787 - Atu0304 0.59 -0.3 296,805 + Atu0304 0.62 -2.1 296,856 + Atu0304 0.71 -0.2 296,872 - Atu0304 0.74 -0.1 296,914 + Atu0304 0.82 +0.1 296,924 - Atu0304 0.83 -1.8 296,924 - Atu0304 0.83 +0.9 296,954 + Atu0304 0.89 -0.7 296,954 + Atu0304 0.89 +0.4 296,958 - Atu0304 0.89 -0.8 296,973 - +0.2 297,086 - -0.1 297,186 - -0.1 297,188 - -1.8 297,213 + +0.2 297,213 + -1.8 297,221 - -0.6 297,251 + -0.5 297,269 + Atu0305 0.11 +0.2 297,277 - Atu0305 0.12 +0.3 297,333 - Atu0305 0.16 -0.3
Or see this region's nucleotide sequence