Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF948

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF947 and GFF948 overlap by 1 nucleotidesGFF948 and GFF949 are separated by 136 nucleotides GFF947 - Valyl-tRNA synthetase (EC 6.1.1.9), at 120,365 to 123,220 GFF947 GFF948 - DNA polymerase III chi subunit (EC 2.7.7.7), at 123,220 to 123,663 GFF948 GFF949 - Cytosol aminopeptidase PepA (EC 3.4.11.1), at 123,800 to 125,311 GFF949 Position (kb) 123 124Strain fitness (log2 ratio) -1 0 1at 123.363 kb on + strand, within GFF948at 123.364 kb on - strand, within GFF948at 123.364 kb on - strand, within GFF948at 123.364 kb on - strand, within GFF948at 123.644 kb on + strandat 123.665 kb on + strandat 123.666 kb on - strandat 123.689 kb on - strandat 123.854 kb on + strandat 123.930 kb on + strandat 123.930 kb on + strandat 123.930 kb on + strandat 123.934 kb on + strandat 123.940 kb on + strandat 124.218 kb on + strand, within GFF949at 124.218 kb on + strand, within GFF949at 124.552 kb on - strand, within GFF949at 124.552 kb on - strand, within GFF949

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
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123,363 + GFF948 0.32 -1.1
123,364 - GFF948 0.32 -0.5
123,364 - GFF948 0.32 -1.4
123,364 - GFF948 0.32 -0.9
123,644 + -0.9
123,665 + -0.6
123,666 - +0.2
123,689 - +0.4
123,854 + +1.3
123,930 + +0.0
123,930 + +0.5
123,930 + -0.6
123,934 + -0.8
123,940 + -0.3
124,218 + GFF949 0.28 -0.3
124,218 + GFF949 0.28 +0.2
124,552 - GFF949 0.50 +0.1
124,552 - GFF949 0.50 +0.2

Or see this region's nucleotide sequence