Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF86 and GFF87 are separated by 176 nucleotides GFF87 and GFF88 are separated by 23 nucleotides GFF88 and GFF89 are separated by 194 nucleotides
GFF86 - Sugar/maltose fermentation stimulation protein homolog, at 71,356 to 72,060
GFF86
GFF87 - C4-type zinc finger protein, DksA/TraR family, at 72,237 to 72,692
GFF87
GFF88 - glutamyl-Q-tRNA synthetase, at 72,716 to 73,657
GFF88
GFF89 - Poly(A) polymerase (EC 2.7.7.19), at 73,852 to 75,216
GFF89
Position (kb) 72
73
74 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 71.754 kb on + strand, within GFF86 at 71.755 kb on - strand, within GFF86 at 71.934 kb on + strand, within GFF86 at 71.940 kb on + strand, within GFF86 at 71.943 kb on - strand, within GFF86 at 71.998 kb on + strand at 72.000 kb on + strand at 72.085 kb on + strand at 72.573 kb on + strand, within GFF87 at 72.711 kb on + strand at 72.712 kb on - strand at 72.776 kb on + strand at 72.777 kb on - strand at 72.777 kb on - strand at 72.852 kb on + strand, within GFF88 at 72.853 kb on - strand, within GFF88 at 72.853 kb on - strand, within GFF88 at 72.988 kb on - strand, within GFF88 at 72.988 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88 at 73.059 kb on - strand, within GFF88 at 73.160 kb on - strand, within GFF88 at 73.276 kb on + strand, within GFF88 at 73.373 kb on + strand, within GFF88 at 73.374 kb on - strand, within GFF88 at 73.375 kb on + strand, within GFF88 at 73.626 kb on + strand at 73.720 kb on - strand at 73.720 kb on - strand at 73.792 kb on + strand at 73.797 kb on + strand at 74.326 kb on + strand, within GFF89 at 74.605 kb on + strand, within GFF89 at 74.655 kb on + strand, within GFF89
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 71,754 + GFF86 0.56 -0.5 71,755 - GFF86 0.57 -0.2 71,934 + GFF86 0.82 -0.6 71,940 + GFF86 0.83 -0.2 71,943 - GFF86 0.83 +0.4 71,998 + +0.2 72,000 + +0.7 72,085 + -0.1 72,573 + GFF87 0.74 -4.2 72,711 + -0.4 72,712 - -0.2 72,776 + +0.1 72,777 - +0.1 72,777 - +0.4 72,852 + GFF88 0.14 -1.4 72,853 - GFF88 0.15 -0.0 72,853 - GFF88 0.15 -0.3 72,988 - GFF88 0.29 +0.1 72,988 - GFF88 0.29 -0.6 73,059 - GFF88 0.36 +1.3 73,059 - GFF88 0.36 +0.2 73,059 - GFF88 0.36 -0.9 73,160 - GFF88 0.47 +0.1 73,276 + GFF88 0.59 -0.6 73,373 + GFF88 0.70 +0.0 73,374 - GFF88 0.70 -0.5 73,375 + GFF88 0.70 +0.0 73,626 + +0.3 73,720 - -2.1 73,720 - -2.8 73,792 + -0.4 73,797 + -0.6 74,326 + GFF89 0.35 -1.3 74,605 + GFF89 0.55 -1.1 74,655 + GFF89 0.59 -0.7
Or see this region's nucleotide sequence