Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF727

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF726 and GFF727 are separated by 10 nucleotidesGFF727 and GFF728 are separated by 32 nucleotides GFF726 - Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase, at 84,213 to 85,154 GFF726 GFF727 - Dipeptide transport ATP-binding protein DppD in protein degradation cluster, at 85,165 to 87,036 GFF727 GFF728 - Dipeptide transport system, periplasmic component in protein degradation cluster, at 87,069 to 88,607 GFF728 Position (kb) 85 86 87 88Strain fitness (log2 ratio) -2 -1 0 1 2at 84.174 kb on + strandat 84.174 kb on + strandat 84.289 kb on + strandat 84.290 kb on - strandat 84.295 kb on - strandat 84.367 kb on + strand, within GFF726at 84.367 kb on + strand, within GFF726at 84.432 kb on + strand, within GFF726at 84.433 kb on - strand, within GFF726at 84.571 kb on + strand, within GFF726at 84.572 kb on - strand, within GFF726at 84.819 kb on - strand, within GFF726at 84.819 kb on - strand, within GFF726at 85.065 kb on - strandat 85.098 kb on + strandat 85.098 kb on + strandat 85.099 kb on - strandat 85.524 kb on - strand, within GFF727at 85.527 kb on + strand, within GFF727at 85.891 kb on + strand, within GFF727at 85.923 kb on - strand, within GFF727at 85.956 kb on + strand, within GFF727at 86.100 kb on + strand, within GFF727at 86.100 kb on + strand, within GFF727at 86.100 kb on + strand, within GFF727at 86.379 kb on + strand, within GFF727at 86.721 kb on + strand, within GFF727at 86.733 kb on + strand, within GFF727at 86.734 kb on - strand, within GFF727at 86.777 kb on - strand, within GFF727at 86.777 kb on - strand, within GFF727at 86.839 kb on + strand, within GFF727at 86.910 kb on - strandat 86.910 kb on - strandat 86.921 kb on + strandat 87.032 kb on + strandat 87.032 kb on + strandat 87.033 kb on - strandat 87.035 kb on - strandat 87.090 kb on - strandat 87.198 kb on + strandat 87.213 kb on - strandat 87.303 kb on + strand, within GFF728at 87.719 kb on + strand, within GFF728at 87.867 kb on - strand, within GFF728at 87.876 kb on + strand, within GFF728at 87.876 kb on + strand, within GFF728at 87.877 kb on - strand, within GFF728at 87.877 kb on - strand, within GFF728at 87.878 kb on + strand, within GFF728at 87.885 kb on - strand, within GFF728at 87.911 kb on + strand, within GFF728at 87.940 kb on - strand, within GFF728

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
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84,174 + -0.8
84,174 + +0.1
84,289 + +0.2
84,290 - +0.3
84,295 - -0.4
84,367 + GFF726 0.16 +0.1
84,367 + GFF726 0.16 +0.3
84,432 + GFF726 0.23 +0.3
84,433 - GFF726 0.23 -0.1
84,571 + GFF726 0.38 +0.1
84,572 - GFF726 0.38 +2.2
84,819 - GFF726 0.64 -0.0
84,819 - GFF726 0.64 -2.5
85,065 - +0.3
85,098 + +0.0
85,098 + -0.1
85,099 - -0.5
85,524 - GFF727 0.19 +0.6
85,527 + GFF727 0.19 -0.4
85,891 + GFF727 0.39 +0.7
85,923 - GFF727 0.40 +0.6
85,956 + GFF727 0.42 +0.0
86,100 + GFF727 0.50 -0.3
86,100 + GFF727 0.50 -0.7
86,100 + GFF727 0.50 -0.7
86,379 + GFF727 0.65 -0.2
86,721 + GFF727 0.83 +0.9
86,733 + GFF727 0.84 -0.1
86,734 - GFF727 0.84 +0.4
86,777 - GFF727 0.86 +0.4
86,777 - GFF727 0.86 +0.3
86,839 + GFF727 0.89 -0.6
86,910 - -0.5
86,910 - -0.4
86,921 + +0.0
87,032 + -0.3
87,032 + -0.7
87,033 - -0.9
87,035 - -0.3
87,090 - +0.0
87,198 + -0.7
87,213 - +0.2
87,303 + GFF728 0.15 +0.1
87,719 + GFF728 0.42 -1.0
87,867 - GFF728 0.52 +0.1
87,876 + GFF728 0.52 +0.4
87,876 + GFF728 0.52 +0.1
87,877 - GFF728 0.53 -0.1
87,877 - GFF728 0.53 +0.0
87,878 + GFF728 0.53 -0.1
87,885 - GFF728 0.53 -0.6
87,911 + GFF728 0.55 +0.2
87,940 - GFF728 0.57 -0.8

Or see this region's nucleotide sequence