Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF694 and GFF695 overlap by 1 nucleotides GFF695 and GFF696 are separated by 229 nucleotides GFF696 and GFF697 are separated by 207 nucleotides
GFF694 - Predicted membrane fusion protein (MFP) component of efflux pump, membrane anchor protein YbhG, at 50,751 to 51,746
GFF694
GFF695 - Transcriptional regulator YbiH, TetR family, at 51,746 to 52,420
GFF695
GFF696 - ATP-dependent RNA helicase RhlE, at 52,650 to 54,014
GFF696
GFF697 - ATP-dependent helicase DinG/Rad3, at 54,222 to 56,366
GFF697
Position (kb) 52
53
54
55 Strain fitness (log2 ratio) -2
-1
0
1 at 51.673 kb on + strand at 51.781 kb on + strand at 51.781 kb on + strand at 51.782 kb on - strand at 51.782 kb on - strand at 51.916 kb on + strand, within GFF695 at 51.935 kb on - strand, within GFF695 at 52.099 kb on + strand, within GFF695 at 52.248 kb on - strand, within GFF695 at 52.277 kb on - strand, within GFF695 at 52.415 kb on - strand at 52.645 kb on + strand at 52.649 kb on + strand at 52.649 kb on + strand at 52.650 kb on - strand at 52.682 kb on + strand at 52.721 kb on + strand at 52.722 kb on - strand at 52.947 kb on - strand, within GFF696 at 53.130 kb on - strand, within GFF696 at 53.523 kb on - strand, within GFF696 at 53.558 kb on + strand, within GFF696 at 53.607 kb on + strand, within GFF696 at 53.608 kb on - strand, within GFF696 at 53.634 kb on + strand, within GFF696 at 53.635 kb on - strand, within GFF696 at 53.635 kb on - strand, within GFF696 at 53.635 kb on - strand, within GFF696 at 53.635 kb on - strand, within GFF696 at 53.638 kb on + strand, within GFF696 at 53.711 kb on + strand, within GFF696 at 53.763 kb on - strand, within GFF696 at 53.906 kb on + strand at 54.074 kb on + strand at 54.074 kb on + strand at 54.075 kb on - strand at 54.075 kb on - strand at 54.151 kb on + strand at 54.266 kb on + strand at 54.296 kb on + strand at 54.411 kb on + strand at 54.573 kb on + strand, within GFF697 at 54.573 kb on + strand, within GFF697 at 54.574 kb on - strand, within GFF697 at 54.786 kb on - strand, within GFF697 at 54.823 kb on - strand, within GFF697 at 54.959 kb on - strand, within GFF697
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 51,673 + -0.4 51,781 + +1.3 51,781 + -1.0 51,782 - -0.4 51,782 - -0.5 51,916 + GFF695 0.25 +0.6 51,935 - GFF695 0.28 -0.0 52,099 + GFF695 0.52 -1.7 52,248 - GFF695 0.74 -0.1 52,277 - GFF695 0.79 -1.3 52,415 - -0.2 52,645 + -2.5 52,649 + -0.7 52,649 + +0.5 52,650 - -0.5 52,682 + -0.6 52,721 + -0.1 52,722 - -0.2 52,947 - GFF696 0.22 +0.3 53,130 - GFF696 0.35 -0.3 53,523 - GFF696 0.64 -0.2 53,558 + GFF696 0.67 -0.5 53,607 + GFF696 0.70 -0.7 53,608 - GFF696 0.70 -0.3 53,634 + GFF696 0.72 -0.1 53,635 - GFF696 0.72 -0.6 53,635 - GFF696 0.72 -0.3 53,635 - GFF696 0.72 -0.4 53,635 - GFF696 0.72 +1.1 53,638 + GFF696 0.72 -0.6 53,711 + GFF696 0.78 +0.2 53,763 - GFF696 0.82 +0.2 53,906 + +0.4 54,074 + +0.4 54,074 + -1.0 54,075 - +0.7 54,075 - +0.6 54,151 + +0.6 54,266 + +0.2 54,296 + -1.6 54,411 + +0.4 54,573 + GFF697 0.16 -0.5 54,573 + GFF697 0.16 -0.4 54,574 - GFF697 0.16 -0.4 54,786 - GFF697 0.26 -0.7 54,823 - GFF697 0.28 +0.2 54,959 - GFF697 0.34 -0.3
Or see this region's nucleotide sequence