Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF688 and GFF689 overlap by 4 nucleotides GFF689 and GFF690 are separated by 132 nucleotides GFF690 and GFF691 overlap by 39 nucleotides
GFF688 - Cardiolipin synthetase (EC 2.7.8.-), at 44,176 to 45,417
GFF688
GFF689 - Endonuclease/Exonuclease/phosphatase family protein, at 45,414 to 46,172
GFF689
GFF690 - Putative inner membrane protein, at 46,305 to 46,715
GFF690
GFF691 - ABC transport system, permease component YbhR, at 46,677 to 47,783
GFF691
Position (kb) 45
46
47 Strain fitness (log2 ratio) -1
0
1
2 at 44.574 kb on - strand, within GFF688 at 44.604 kb on + strand, within GFF688 at 44.605 kb on - strand, within GFF688 at 44.979 kb on - strand, within GFF688 at 45.013 kb on - strand, within GFF688 at 45.013 kb on - strand, within GFF688 at 45.031 kb on - strand, within GFF688 at 45.031 kb on - strand, within GFF688 at 45.051 kb on + strand, within GFF688 at 45.097 kb on - strand, within GFF688 at 45.215 kb on - strand, within GFF688 at 45.217 kb on + strand, within GFF688 at 45.217 kb on + strand, within GFF688 at 45.217 kb on + strand, within GFF688 at 45.230 kb on - strand, within GFF688 at 45.518 kb on + strand, within GFF689 at 45.519 kb on - strand, within GFF689 at 45.589 kb on - strand, within GFF689 at 45.712 kb on - strand, within GFF689 at 45.712 kb on - strand, within GFF689 at 45.751 kb on - strand, within GFF689 at 45.802 kb on + strand, within GFF689 at 45.907 kb on + strand, within GFF689 at 46.208 kb on - strand at 46.208 kb on - strand at 46.436 kb on - strand, within GFF690 at 46.706 kb on - strand at 46.805 kb on + strand, within GFF691 at 46.911 kb on - strand, within GFF691 at 46.911 kb on - strand, within GFF691 at 46.993 kb on + strand, within GFF691
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 44,574 - GFF688 0.32 +0.4 44,604 + GFF688 0.34 +0.2 44,605 - GFF688 0.35 +0.2 44,979 - GFF688 0.65 -0.3 45,013 - GFF688 0.67 -0.3 45,013 - GFF688 0.67 +0.1 45,031 - GFF688 0.69 +1.1 45,031 - GFF688 0.69 -0.4 45,051 + GFF688 0.70 -0.4 45,097 - GFF688 0.74 +0.3 45,215 - GFF688 0.84 -0.0 45,217 + GFF688 0.84 +0.2 45,217 + GFF688 0.84 -0.3 45,217 + GFF688 0.84 +1.2 45,230 - GFF688 0.85 +0.2 45,518 + GFF689 0.14 -0.5 45,519 - GFF689 0.14 -0.2 45,589 - GFF689 0.23 -0.1 45,712 - GFF689 0.39 -0.1 45,712 - GFF689 0.39 -0.0 45,751 - GFF689 0.44 -1.1 45,802 + GFF689 0.51 -0.1 45,907 + GFF689 0.65 -1.1 46,208 - -0.2 46,208 - +0.2 46,436 - GFF690 0.32 +0.1 46,706 - +0.2 46,805 + GFF691 0.12 +0.8 46,911 - GFF691 0.21 +0.0 46,911 - GFF691 0.21 -0.5 46,993 + GFF691 0.29 +2.6
Or see this region's nucleotide sequence