Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF55 and GFF56 are separated by 29 nucleotides GFF56 and GFF57 are separated by 36 nucleotides
GFF55 - [Protein-PII] uridylyltransferase (EC 2.7.7.59), at 36,274 to 38,946
GFF55
GFF56 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 38,976 to 39,800
GFF56
GFF57 - Permeases of the major facilitator superfamily, at 39,837 to 41,138
GFF57
Position (kb) 38
39
40 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 38.094 kb on + strand, within GFF55 at 38.333 kb on + strand, within GFF55 at 38.471 kb on + strand, within GFF55 at 38.523 kb on + strand, within GFF55 at 38.523 kb on + strand, within GFF55 at 38.530 kb on - strand, within GFF55 at 38.616 kb on + strand, within GFF55 at 38.667 kb on + strand, within GFF55 at 38.877 kb on + strand at 38.878 kb on - strand at 38.880 kb on + strand at 38.957 kb on + strand at 38.959 kb on + strand at 39.792 kb on - strand at 39.938 kb on + strand at 39.944 kb on - strand at 40.030 kb on + strand, within GFF57 at 40.035 kb on + strand, within GFF57 at 40.036 kb on - strand, within GFF57 at 40.106 kb on + strand, within GFF57 at 40.249 kb on - strand, within GFF57 at 40.249 kb on - strand, within GFF57 at 40.287 kb on + strand, within GFF57 at 40.472 kb on + strand, within GFF57 at 40.532 kb on + strand, within GFF57 at 40.532 kb on + strand, within GFF57 at 40.532 kb on + strand, within GFF57 at 40.534 kb on + strand, within GFF57 at 40.534 kb on + strand, within GFF57 at 40.534 kb on + strand, within GFF57 at 40.535 kb on - strand, within GFF57 at 40.535 kb on - strand, within GFF57 at 40.535 kb on - strand, within GFF57 at 40.536 kb on + strand, within GFF57 at 40.537 kb on - strand, within GFF57 at 40.537 kb on - strand, within GFF57 at 40.626 kb on + strand, within GFF57 at 40.629 kb on - strand, within GFF57 at 40.632 kb on + strand, within GFF57 at 40.714 kb on + strand, within GFF57
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 38,094 + GFF55 0.68 -1.8 38,333 + GFF55 0.77 -1.3 38,471 + GFF55 0.82 -2.9 38,523 + GFF55 0.84 -1.6 38,523 + GFF55 0.84 -0.9 38,530 - GFF55 0.84 -2.3 38,616 + GFF55 0.88 -0.7 38,667 + GFF55 0.90 -1.1 38,877 + +1.5 38,878 - -0.9 38,880 + +1.1 38,957 + +0.7 38,959 + +0.5 39,792 - -5.8 39,938 + -0.0 39,944 - -0.1 40,030 + GFF57 0.15 +0.3 40,035 + GFF57 0.15 +1.1 40,036 - GFF57 0.15 +0.4 40,106 + GFF57 0.21 +1.1 40,249 - GFF57 0.32 +1.5 40,249 - GFF57 0.32 -0.1 40,287 + GFF57 0.35 +1.0 40,472 + GFF57 0.49 +0.6 40,532 + GFF57 0.53 +0.0 40,532 + GFF57 0.53 -0.5 40,532 + GFF57 0.53 +0.1 40,534 + GFF57 0.54 +0.1 40,534 + GFF57 0.54 -0.3 40,534 + GFF57 0.54 -0.0 40,535 - GFF57 0.54 +0.6 40,535 - GFF57 0.54 +1.4 40,535 - GFF57 0.54 +0.8 40,536 + GFF57 0.54 +0.1 40,537 - GFF57 0.54 +0.6 40,537 - GFF57 0.54 +0.3 40,626 + GFF57 0.61 -0.3 40,629 - GFF57 0.61 +0.0 40,632 + GFF57 0.61 -0.2 40,714 + GFF57 0.67 -0.5
Or see this region's nucleotide sequence