Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF536 and GFF537 are separated by 80 nucleotides GFF537 and GFF538 are separated by 141 nucleotides GFF538 and GFF539 are separated by 21 nucleotides GFF539 and GFF540 overlap by 11 nucleotides
GFF536 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 268,436 to 270,634
GFF536
GFF537 - probable enzyme yhhN, at 270,715 to 271,341
GFF537
GFF538 - Putative receptor, at 271,483 to 271,854
GFF538
GFF539 - Putative inner membrane protein, at 271,876 to 272,145
GFF539
GFF540 - 16S rRNA (guanine(966)-N(2))-methyltransferase (EC 2.1.1.171), at 272,135 to 272,731
GFF540
Position (kb) 271
272 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 270.485 kb on - strand at 270.487 kb on - strand at 270.554 kb on - strand at 270.566 kb on + strand at 270.634 kb on + strand at 270.635 kb on - strand at 270.635 kb on - strand at 270.635 kb on - strand at 270.712 kb on + strand at 270.716 kb on + strand at 270.722 kb on + strand at 270.826 kb on - strand, within GFF537 at 270.942 kb on + strand, within GFF537 at 270.943 kb on - strand, within GFF537 at 270.947 kb on - strand, within GFF537 at 270.958 kb on + strand, within GFF537 at 270.959 kb on - strand, within GFF537 at 271.074 kb on - strand, within GFF537 at 271.109 kb on + strand, within GFF537 at 271.110 kb on - strand, within GFF537 at 271.234 kb on + strand, within GFF537 at 271.235 kb on - strand, within GFF537 at 271.235 kb on - strand, within GFF537 at 271.252 kb on - strand, within GFF537 at 271.284 kb on - strand at 271.298 kb on + strand at 271.298 kb on + strand at 271.298 kb on + strand at 271.378 kb on + strand at 271.404 kb on + strand at 271.434 kb on - strand at 271.434 kb on - strand at 271.434 kb on - strand at 271.435 kb on + strand at 271.436 kb on - strand at 271.595 kb on + strand, within GFF538 at 271.641 kb on + strand, within GFF538 at 271.689 kb on + strand, within GFF538 at 271.696 kb on + strand, within GFF538 at 271.802 kb on - strand, within GFF538 at 271.853 kb on - strand at 272.010 kb on + strand, within GFF539 at 272.056 kb on - strand, within GFF539 at 272.088 kb on + strand, within GFF539 at 272.089 kb on - strand, within GFF539 at 272.173 kb on - strand at 272.264 kb on + strand, within GFF540 at 272.265 kb on - strand, within GFF540 at 272.269 kb on - strand, within GFF540 at 272.269 kb on - strand, within GFF540 at 272.315 kb on + strand, within GFF540 at 272.405 kb on - strand, within GFF540 at 272.576 kb on + strand, within GFF540 at 272.599 kb on + strand, within GFF540 at 272.599 kb on + strand, within GFF540 at 272.666 kb on - strand, within GFF540 at 272.827 kb on + strand at 272.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 270,485 - -0.2 270,487 - +0.4 270,554 - -0.2 270,566 + +0.1 270,634 + -0.6 270,635 - +0.7 270,635 - -0.3 270,635 - -0.3 270,712 + +0.5 270,716 + -0.6 270,722 + +1.5 270,826 - GFF537 0.18 -0.2 270,942 + GFF537 0.36 -0.3 270,943 - GFF537 0.36 +0.6 270,947 - GFF537 0.37 +0.3 270,958 + GFF537 0.39 +0.5 270,959 - GFF537 0.39 -0.8 271,074 - GFF537 0.57 -0.3 271,109 + GFF537 0.63 -0.1 271,110 - GFF537 0.63 +0.2 271,234 + GFF537 0.83 -0.0 271,235 - GFF537 0.83 +1.5 271,235 - GFF537 0.83 +0.5 271,252 - GFF537 0.86 -0.8 271,284 - +0.1 271,298 + -0.3 271,298 + +0.1 271,298 + -0.6 271,378 + +0.9 271,404 + +0.1 271,434 - +0.2 271,434 - +0.1 271,434 - +0.1 271,435 + +0.7 271,436 - -3.8 271,595 + GFF538 0.30 +0.6 271,641 + GFF538 0.42 +0.3 271,689 + GFF538 0.55 +0.7 271,696 + GFF538 0.57 +1.1 271,802 - GFF538 0.86 +0.4 271,853 - -0.1 272,010 + GFF539 0.50 +1.1 272,056 - GFF539 0.67 -1.9 272,088 + GFF539 0.79 +0.7 272,089 - GFF539 0.79 +0.7 272,173 - +1.4 272,264 + GFF540 0.22 +0.8 272,265 - GFF540 0.22 -0.7 272,269 - GFF540 0.22 +0.4 272,269 - GFF540 0.22 +0.2 272,315 + GFF540 0.30 -0.1 272,405 - GFF540 0.45 +0.3 272,576 + GFF540 0.74 +0.6 272,599 + GFF540 0.78 +0.6 272,599 + GFF540 0.78 +0.6 272,666 - GFF540 0.89 +0.6 272,827 + -0.6 272,827 + -1.1
Or see this region's nucleotide sequence