Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4854 and GFF4855 are separated by 91 nucleotides GFF4855 and GFF4856 are separated by 16 nucleotides GFF4856 and GFF4857 are separated by 153 nucleotides
GFF4854 - DNA polymerase I (EC 2.7.7.7), at 153,623 to 154,378
GFF4854
GFF4855 - Lactaldehyde reductase (EC 1.1.1.77), at 154,470 to 155,618
GFF4855
GFF4856 - L-fuculose phosphate aldolase (EC 4.1.2.17), at 155,635 to 156,282
GFF4856
GFF4857 - FIG01045182: hypothetical protein, at 156,436 to 156,603
GFF4857
Position (kb) 154
155
156 Strain fitness (log2 ratio) -1
0
1 at 153.542 kb on + strand at 153.555 kb on - strand at 153.591 kb on - strand at 153.734 kb on - strand, within GFF4854 at 153.852 kb on + strand, within GFF4854 at 153.856 kb on + strand, within GFF4854 at 153.857 kb on - strand, within GFF4854 at 154.011 kb on - strand, within GFF4854 at 154.102 kb on - strand, within GFF4854 at 154.148 kb on - strand, within GFF4854 at 154.148 kb on - strand, within GFF4854 at 154.336 kb on - strand at 154.362 kb on + strand at 154.471 kb on + strand at 154.487 kb on - strand at 154.637 kb on + strand, within GFF4855 at 154.661 kb on + strand, within GFF4855 at 154.880 kb on - strand, within GFF4855 at 154.967 kb on + strand, within GFF4855 at 154.968 kb on - strand, within GFF4855 at 154.971 kb on + strand, within GFF4855 at 154.971 kb on + strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 154.972 kb on - strand, within GFF4855 at 155.007 kb on - strand, within GFF4855 at 155.007 kb on - strand, within GFF4855 at 155.009 kb on - strand, within GFF4855 at 155.018 kb on + strand, within GFF4855 at 155.167 kb on + strand, within GFF4855 at 155.217 kb on - strand, within GFF4855 at 155.288 kb on + strand, within GFF4855 at 155.351 kb on - strand, within GFF4855 at 155.647 kb on - strand at 155.738 kb on - strand, within GFF4856 at 155.738 kb on - strand, within GFF4856 at 155.742 kb on - strand, within GFF4856 at 155.946 kb on - strand, within GFF4856 at 155.946 kb on - strand, within GFF4856 at 156.007 kb on - strand, within GFF4856
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 153,542 + -0.0 153,555 - -0.2 153,591 - +0.5 153,734 - GFF4854 0.15 +0.4 153,852 + GFF4854 0.30 +1.3 153,856 + GFF4854 0.31 -0.2 153,857 - GFF4854 0.31 +0.3 154,011 - GFF4854 0.51 -0.1 154,102 - GFF4854 0.63 -0.1 154,148 - GFF4854 0.69 +0.2 154,148 - GFF4854 0.69 -0.2 154,336 - -1.3 154,362 + -0.4 154,471 + -0.7 154,487 - +0.1 154,637 + GFF4855 0.15 +0.6 154,661 + GFF4855 0.17 +0.6 154,880 - GFF4855 0.36 +0.4 154,967 + GFF4855 0.43 +0.3 154,968 - GFF4855 0.43 +0.3 154,971 + GFF4855 0.44 -0.1 154,971 + GFF4855 0.44 +0.3 154,972 - GFF4855 0.44 -0.0 154,972 - GFF4855 0.44 -0.3 154,972 - GFF4855 0.44 -0.0 154,972 - GFF4855 0.44 +0.9 154,972 - GFF4855 0.44 +0.3 155,007 - GFF4855 0.47 -0.1 155,007 - GFF4855 0.47 -0.4 155,009 - GFF4855 0.47 +0.5 155,018 + GFF4855 0.48 +0.1 155,167 + GFF4855 0.61 -0.4 155,217 - GFF4855 0.65 +0.8 155,288 + GFF4855 0.71 +0.9 155,351 - GFF4855 0.77 +0.4 155,647 - +0.6 155,738 - GFF4856 0.16 +0.1 155,738 - GFF4856 0.16 +0.3 155,742 - GFF4856 0.17 +0.4 155,946 - GFF4856 0.48 +1.0 155,946 - GFF4856 0.48 +1.5 156,007 - GFF4856 0.57 +0.6
Or see this region's nucleotide sequence