Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4852

Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4850 and GFF4851 overlap by 4 nucleotidesGFF4851 and GFF4852 are separated by 448 nucleotidesGFF4852 and GFF4853 are separated by 58 nucleotides GFF4850 - Decarboxylase family protein, at 148,808 to 150,172 GFF4850 GFF4851 - hypothetical protein, at 150,169 to 150,288 GFF4851 GFF4852 - Serine transporter, at 150,737 to 152,026 GFF4852 GFF4853 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 152,085 to 153,452 GFF4853 Position (kb) 150 151 152 153Strain fitness (log2 ratio) -1 0 1at 149.854 kb on + strand, within GFF4850at 149.915 kb on - strand, within GFF4850at 150.143 kb on + strandat 150.143 kb on + strandat 150.143 kb on + strandat 150.143 kb on + strandat 150.245 kb on - strand, within GFF4851at 150.462 kb on + strandat 150.463 kb on - strandat 150.517 kb on - strandat 150.522 kb on - strandat 150.543 kb on + strandat 150.760 kb on + strandat 150.761 kb on - strandat 151.039 kb on + strand, within GFF4852at 151.050 kb on - strand, within GFF4852at 151.100 kb on - strand, within GFF4852at 151.235 kb on - strand, within GFF4852at 151.235 kb on - strand, within GFF4852at 151.281 kb on - strand, within GFF4852at 151.672 kb on + strand, within GFF4852at 151.672 kb on + strand, within GFF4852at 151.697 kb on + strand, within GFF4852at 151.698 kb on - strand, within GFF4852at 151.720 kb on + strand, within GFF4852at 151.910 kb on + strandat 151.910 kb on + strandat 151.910 kb on + strandat 151.911 kb on - strandat 151.911 kb on - strandat 151.965 kb on + strandat 151.965 kb on + strandat 151.978 kb on + strandat 152.043 kb on + strandat 152.064 kb on + strandat 152.065 kb on - strandat 152.084 kb on + strandat 152.091 kb on - strandat 152.096 kb on - strandat 152.096 kb on - strandat 152.282 kb on + strand, within GFF4853at 152.371 kb on + strand, within GFF4853at 152.469 kb on - strand, within GFF4853at 152.511 kb on + strand, within GFF4853at 152.512 kb on - strand, within GFF4853at 152.515 kb on - strand, within GFF4853at 152.879 kb on + strand, within GFF4853at 152.926 kb on + strand, within GFF4853at 152.942 kb on + strand, within GFF4853at 152.958 kb on - strand, within GFF4853at 152.958 kb on - strand, within GFF4853at 152.980 kb on - strand, within GFF4853at 152.980 kb on - strand, within GFF4853at 152.983 kb on - strand, within GFF4853

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
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149,854 + GFF4850 0.77 +0.5
149,915 - GFF4850 0.81 +0.1
150,143 + +0.9
150,143 + +0.1
150,143 + -0.2
150,143 + -0.3
150,245 - GFF4851 0.63 -0.4
150,462 + +0.2
150,463 - +0.5
150,517 - +0.2
150,522 - -0.1
150,543 + +0.3
150,760 + -0.8
150,761 - +0.6
151,039 + GFF4852 0.23 +0.6
151,050 - GFF4852 0.24 -0.1
151,100 - GFF4852 0.28 +0.3
151,235 - GFF4852 0.39 +1.3
151,235 - GFF4852 0.39 +0.7
151,281 - GFF4852 0.42 +0.3
151,672 + GFF4852 0.72 +0.2
151,672 + GFF4852 0.72 -0.5
151,697 + GFF4852 0.74 -0.3
151,698 - GFF4852 0.74 +0.6
151,720 + GFF4852 0.76 +0.4
151,910 + +0.2
151,910 + +0.5
151,910 + +0.7
151,911 - +0.1
151,911 - +1.0
151,965 + +0.2
151,965 + +0.0
151,978 + -1.2
152,043 + -0.0
152,064 + +0.1
152,065 - -0.0
152,084 + +0.5
152,091 - +0.1
152,096 - +0.2
152,096 - +0.1
152,282 + GFF4853 0.14 +0.7
152,371 + GFF4853 0.21 +0.6
152,469 - GFF4853 0.28 -0.2
152,511 + GFF4853 0.31 +1.3
152,512 - GFF4853 0.31 +1.7
152,515 - GFF4853 0.31 +0.6
152,879 + GFF4853 0.58 +0.3
152,926 + GFF4853 0.61 +0.4
152,942 + GFF4853 0.63 -0.4
152,958 - GFF4853 0.64 -1.5
152,958 - GFF4853 0.64 +0.1
152,980 - GFF4853 0.65 -0.0
152,980 - GFF4853 0.65 +1.7
152,983 - GFF4853 0.66 +0.3

Or see this region's nucleotide sequence