Experiment: LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4850 and GFF4851 overlap by 4 nucleotides GFF4851 and GFF4852 are separated by 448 nucleotides GFF4852 and GFF4853 are separated by 58 nucleotides
GFF4850 - Decarboxylase family protein, at 148,808 to 150,172
GFF4850
GFF4851 - hypothetical protein, at 150,169 to 150,288
GFF4851
GFF4852 - Serine transporter, at 150,737 to 152,026
GFF4852
GFF4853 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 152,085 to 153,452
GFF4853
Position (kb)
150
151
152
153 Strain fitness (log2 ratio)
-1
0
1 at 149.854 kb on + strand, within GFF4850 at 149.915 kb on - strand, within GFF4850 at 150.143 kb on + strand at 150.143 kb on + strand at 150.143 kb on + strand at 150.143 kb on + strand at 150.245 kb on - strand, within GFF4851 at 150.462 kb on + strand at 150.463 kb on - strand at 150.517 kb on - strand at 150.522 kb on - strand at 150.543 kb on + strand at 150.760 kb on + strand at 150.761 kb on - strand at 151.039 kb on + strand, within GFF4852 at 151.050 kb on - strand, within GFF4852 at 151.100 kb on - strand, within GFF4852 at 151.235 kb on - strand, within GFF4852 at 151.235 kb on - strand, within GFF4852 at 151.281 kb on - strand, within GFF4852 at 151.672 kb on + strand, within GFF4852 at 151.672 kb on + strand, within GFF4852 at 151.697 kb on + strand, within GFF4852 at 151.698 kb on - strand, within GFF4852 at 151.720 kb on + strand, within GFF4852 at 151.910 kb on + strand at 151.910 kb on + strand at 151.910 kb on + strand at 151.911 kb on - strand at 151.911 kb on - strand at 151.965 kb on + strand at 151.965 kb on + strand at 151.978 kb on + strand at 152.043 kb on + strand at 152.064 kb on + strand at 152.065 kb on - strand at 152.084 kb on + strand at 152.091 kb on - strand at 152.096 kb on - strand at 152.096 kb on - strand at 152.282 kb on + strand, within GFF4853 at 152.371 kb on + strand, within GFF4853 at 152.469 kb on - strand, within GFF4853 at 152.511 kb on + strand, within GFF4853 at 152.512 kb on - strand, within GFF4853 at 152.515 kb on - strand, within GFF4853 at 152.879 kb on + strand, within GFF4853 at 152.926 kb on + strand, within GFF4853 at 152.942 kb on + strand, within GFF4853 at 152.958 kb on - strand, within GFF4853 at 152.958 kb on - strand, within GFF4853 at 152.980 kb on - strand, within GFF4853 at 152.980 kb on - strand, within GFF4853 at 152.983 kb on - strand, within GFF4853
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 0.001875 MOI remove 149,854 + GFF4850 0.77 +0.5 149,915 - GFF4850 0.81 +0.1 150,143 + +0.9 150,143 + +0.1 150,143 + -0.2 150,143 + -0.3 150,245 - GFF4851 0.63 -0.4 150,462 + +0.2 150,463 - +0.5 150,517 - +0.2 150,522 - -0.1 150,543 + +0.3 150,760 + -0.8 150,761 - +0.6 151,039 + GFF4852 0.23 +0.6 151,050 - GFF4852 0.24 -0.1 151,100 - GFF4852 0.28 +0.3 151,235 - GFF4852 0.39 +1.3 151,235 - GFF4852 0.39 +0.7 151,281 - GFF4852 0.42 +0.3 151,672 + GFF4852 0.72 +0.2 151,672 + GFF4852 0.72 -0.5 151,697 + GFF4852 0.74 -0.3 151,698 - GFF4852 0.74 +0.6 151,720 + GFF4852 0.76 +0.4 151,910 + +0.2 151,910 + +0.5 151,910 + +0.7 151,911 - +0.1 151,911 - +1.0 151,965 + +0.2 151,965 + +0.0 151,978 + -1.2 152,043 + -0.0 152,064 + +0.1 152,065 - -0.0 152,084 + +0.5 152,091 - +0.1 152,096 - +0.2 152,096 - +0.1 152,282 + GFF4853 0.14 +0.7 152,371 + GFF4853 0.21 +0.6 152,469 - GFF4853 0.28 -0.2 152,511 + GFF4853 0.31 +1.3 152,512 - GFF4853 0.31 +1.7 152,515 - GFF4853 0.31 +0.6 152,879 + GFF4853 0.58 +0.3 152,926 + GFF4853 0.61 +0.4 152,942 + GFF4853 0.63 -0.4 152,958 - GFF4853 0.64 -1.5 152,958 - GFF4853 0.64 +0.1 152,980 - GFF4853 0.65 -0.0 152,980 - GFF4853 0.65 +1.7 152,983 - GFF4853 0.66 +0.3
Or see this region's nucleotide sequence